The Journal of Biological Chemistry is a publication of the
@ASBMB
. Publishing research motivated by biology, enabled by chemistry. EiC:
@ChiefEditorJBC
The latest JBC cover shows an electron micrograph (background) of FtsZ filaments from Spiroplasma melliferum, a cell wall-less bacteria, and depicts that extended monomers and domain-swapped dimers are incompatible to form filaments.
#ASBMBJournals
Interestingly, ALL early papers on HIF and its role on O2 sensing by
@NobelPrize
Semenza and colleagues were published in non-glamour society journals, including JBC and Molecular and Cellular Biology
Happy 40th birthday to the Western Blot! At the JBC, our data integrity team performs due diligence to ensure that that duplicated or manipulated blots (we publish so many blots, you guys) don't wind up in publication
Can you tell how this image was manipulated? Here are your choices:
A.A lane was reused.
B.A band was reused.
C.The gel was spliced.
D.All of the above.
Check the replies on Monday for the answer!
#ForensicsFriday
Because we value data integrity and transparency, our staff will be looking into these votes before we declare a champion. Stay tuned for further updates for
#JBCMethodsMadness
Congratulations to
#TeamCryo
for being crowned the first-ever
#JBCMethodsMadness
champion! The final showdown was hard-fought until the end, and we would like to thank all of those who participated and followed along for the finals and throughout the tournament.
We want to congratulate Katalin Karikó (
@kkariko
) and Drew Weissman (
@WeissmanLab
) today for winning the 2023 Nobel Prize in physiology or medicine! Check out their work in JBC cited by the Nobel Committee:
We are excited to announce that
@jbiolchem
,
@molcellprot
and
@jlipidres
are transitioning to gold open access, making
@ASBMB
journals freely available to all starting in January 2021. Learn more about this decision here:
JBC is proud to present
#JBCMethodsMadness
! 16 techniques, 15 matchups, one champion method.
Download a bracket and make your predictions for a chance to win a $50 Amazon gift card. Brackets and more info available here:
JBC is pleased to announce the 2nd annual
#JBCMethodsMadness
tournament! 16 techniques, 15 matchups, one champion method.
Join the fun by submitting your predictions for a chance to win a $100 Amazon gift card. Brackets and more info available here:
Here, Schmidt et al. provide considerations for the design and interpretation of bioenergetics experiments, including how to address the outlined challenges and potential pitfalls ―
#JBCReviews
@CAS_mitolab
@ECUBrodySOM
@KFW_Lab
Here's the last matchup of the
#FreeEnergyFour
. Who will face
#TeamCryo
in the finals?
ROUND 3:
#TeamMassSpec
vs.
#TeamXRC
Who should advance to the next round? Let us know in the comments and retweet to help spread the word!
New in JBC press: "Scientists' observations illustrate the importance of muscle pyruvate dehydrogenase (PDH) in maintaining metabolic flexibility and preventing the development of metabolic disorders."
Learn more:
Honestly living for the outrage in the comments of
@jbiolchem
's twitter right now. Didn't think
#JBCMethodsMadness
could result in such drama, but it is hilarious
We cannot confirm or deny if there was any foul play in the
#JBCMethodsMadness
championship. The tweet analytics do not to track individual voters.
We will scrap that polling data and hold a more secure vote on
@ASBMB
's website tomorrow at 12 p.m. ET:
The final stage is set.
#TeamMassSpec
charged past
#TeamPCR
, and
#TeamCryo
put
#TeamCRISPR
in the deep–freeze. There's one matchup left and only one can be crowned
#JBCMethodsMadness
champion.
Mass Spec vs. Cryo–EM – voting begins on Monday at 12PM! Who ya got winning it all?
Happy birthday, PDB (
@PDBeurope
)!
To commemorate the PDB's 50th anniversary, JBC assembled a thematic review series, How the Protein Data Bank changed biology and a virtual collection, The PDB in JBC.
You can read these collections and more here:
Raducanu et al. devise an approach, UVHis-PAGE, to detect His-tagged proteins in PAGE and blot membranes using a UV transilluminator as an excitation source, and the results can be visually inspected by the naked eye.
#MethodsandResources
@KAUST_News
Congratulations to the
#JBCMethodsMadness
Bracket Challenge winner Dietrich Volmer (
@realBioMassSpec
). Dietrich only had one incorrect selection! Along with the glory of being the bracket champ, he'll receive a $100 Amazon gift card.
Thanks for playing, everyone!
In this
#JBCReview
, Hawkins and Davies summarize new findings about oxidant-induced protein modifications, and highlight methods for detecting such modifications.
"Detection, identification and quantification of oxidative protein modifications":
After a wild first week, it's time to kickoff the first
#EnergeticEight
matchup
ROUND 2:
#TeamNMR
vs.
#TeamXRC
Who should advance to the next round? Let us know in the comments and retweet to help spread the word!
#JBCMethodsMadness
C. elegans daf-2 mutants can live up to 40 days.
JBC takes only 17 days on average to reach a first decision about your paper.
Learn more about fast, quality review at
Raducanu et al. devise an approach, UVHis-PAGE, to detect His-tagged proteins in PAGE and blot membranes using a UV transilluminator as an excitation source, and the results can be visually inspected by the naked eye.
#MethodsandResources
@KAUST_News
Grba et al. report the
#CryoEM
structure of mouse complex I with an extremely tight-binding natural-product acetogenin inhibitor, which resembles the native substrate, bound along the full length of the expected ubiquinone-binding channel —
@MRC_MBU
Check out this paper authored by JBC Associate Editor Ruma Banerjee and colleagues —
@umichmedicine
@LyssiotisLab
"A redox cycle with complex II prioritizes sulfide quinone oxidoreductase dependent H2S oxidation"
The Scientific 16 have been whittled down to an Energetic 8! This updated bracket shows the winners and points awarded for each correct choice. Top 10 standings for bracket submissions will be provided soon! Matchups resume next week starting on Monday morning.
#JBCMethodsMadness
Check out JBC's May cover: "Illuminating GPCR activation and trafficking using genetically encoded synthetic intrabodies against β-arrestin." Credit: Jagannath Maharana.
Learn more in this article by Baidya et al.: .
Dr. John K. Haynes discovered the difference in amino acid sequence between normal and sickle-cell hemoglobin. A Morehouse College alum (class of ’64), he returned as an associate prof, became a dean, still teaches there.
#BlackHistoryMonth
#BlackandSTEM
"(T)here may be an immense diversity of animal small molecules and biosynthetic enzymes awaiting discovery." The biosynthetic diversity of the animal world
@UUtah
@uofu_science
It's
#WorldMicrobiomeDay
and we have a new virtual issue that celebrates all things microbiome, including the diverse molecules, chemical reactions, and interspecies crosstalk found in the gut. We hope you enjoy it and remember to
#mindyourmicrobes
!
#JBCMethodsMadness
finished with a bang! In the end, one team was crowned champion and TWO bracket submissions tied for 1st place! Read about the winners and highlights from the final matchup in the latest article published in
@ASBMB
Today!
Do et al. explore mechanisms of bacterial wall synthesis and tools that have opened new avenues in the study of bacteria.
Paper in Press: "Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes"
The gestational period for Mus Musculus is 19 – 21 days.
JBC takes only 17 days on average to reach a first decision about your paper.
Learn more about fast, quality review at
Congratulations to associate editor Ruma Banerjee on her ASBMB-Merck award in recognition of outstanding contributions to biochemistry and molecular biology!
Researchers from
@UAlberta
have shown that Remdesivir, a drug originally developed to treat Ebola, inhibits replication of SARS-CoV-2 and shows promise as a broad spectrum pharmaceutical treatment for coronaviruses. –
#COVID19
#Enzymology
#coronavirus
This virtual issue simultaneously celebrates progress made in regards to the participation of
#WomenInStem
and serves as a reminder that we have much work still to do to fully include women, and particularly women of color, in the scientific endeavor.
"These findings provide a first view of an integrated molecular architecture for the major energy-generating pathways in mitochondria"
#openaccess
#metabolism
Another week, another edition of
#ForensicsFriday
.
Would you flag this paper if it were under review?
A. Yes, the data have been fabricated.
B. No, looks fine to me.
Paper in Press:
#Metabolism
- "A synthetic lethal drug combination mimics glucose deprivation-induced cancer cell death in the presence of glucose":
@USC
This JBC review on the 3D cryo-electron microscopic imaging of bacterial flagella discusses novel structures and mechanistic insights derived from such high-resolution studies, advancing our understanding of each one of the three major flagellar segments.
BREAKING NEWS:
The 2020
#NobelPrize
in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”
Understanding the physical basis of memory: Molecular mechanisms of the engram
@Clrar
&
@TJRyan_77
review the current understanding of memory formation at a molecular and cellular level by focusing on insights provided using engram technology.
@tcddublin
For years, researchers wondered why water transport seems to occur in some tissues but not in others. Read how Peter Agre solved the mystery of leaky membranes:
#JBCClassics
On Monday, 14 methods watch from the sidelines as
#TeamCryo
battles
#TeamMassSpec
in the
#JBCMethodsMadness
championship. Voting begins at 12 P.M.
Check out this thread for a recap of the tournament matchups so far!
Neurotoxic huntingtin aggregates form three distinct phases, and profilin binds to only one of them. Understanding phase separation might be important to treat diseases caused by aggregation-prone proteins.
#MassSpec
originated during the hunt for the electron. J. J. Thomson and his assistant E. Everett built the first iteration of a mass spectrometer and indirectly measured the mass of an electron, winning Thomson a
@NobelPrize
in 1906.
#JBCMethodsMadness
#TeamMassSpec
gets a year of bragging rights and championship apparel! A portion of the proceeds will go toward
@ASBMB
’s Marion B. Sewer Distinguished Scholarship for Undergraduates.
You can purchase a shirt here:
in this week's JBC Review, Jake F. Watson and Javier García-Nafría summarize the historical trajectory of molecular cloning and collate recent reports to provide a roadmap for its optimal use
#TeamMassSpec
has been a powerhouse and they haven't peaked yet! While
#TeamXRC
kept their matchup closer than any other competitor this year, their support failed to crystallize, causing their chances to scatter.
#TeamMassSpec
moves on to the championship!
#JBCMethodsMadness
Mitochondrial lipids are critical for assembly of the electron transport chain complexes and for effective responses to mitochondrial damage. This minireview focuses on mitochondrial lipid signaling mediated by lipid-protein interactions.
#RedoxSignaling
Heme-binding proteins in nature are helical. Researchers engineered a hairpin-shaped heme-binding peptide, expanding the design space of heme-binding proteins.
It's
#ForensicsFriday
! You are reviewing a paper where 3 siRNAs were tested to knockdown Protein X. You ...
A. are amazed at their 100% efficiency and can’t wait to try it out
B. suggest that they verify with RT-qPCR
C. contact the handling editor because of data irregularity
Dr. Margaret Ellen Mayo Tolbert published 3 JBC articles on her graduate work, describing signal transduction in the rat liver, in the '70s. She went on to a distinguished career in science leadership, directing programs at NSF, ANL and the DoE.
#BlackHistoryMonth
#BlackandSTEM
We've reached 10,000 followers! Thanks to all the science lovers out there keeping up with research published in JBC. We'll keep the quality content coming!
Day 2 of
#JBCMethodsMadness
begins with the defending champ facing off against a tournament rookie!
ROUND 1:
#TeamCryo
vs.
#TeamMachine
Who should advance to the next round? Let us know in the comments and retweet to help spread the word!
In this joint virtual issue, we have selected 27 articles published in JBC and
@jlipidres
over the past century to celebrate the 100th anniversary of the discovery of insulin and the research triumphs that followed ―
#DiabetesAwarenessMonth
Congratulations to
@BMB_PSU
professor Craig Cameron (
@CameronLabPSU
) who has just been appointed as a new Associate Editor for the JBC! Cameron will officially begin his duties as an AE in the spring
We were thrilled to see so many extraordinary scientists from around the World engaging with us and one another, and wish you all the best in your research. Stay safe and healthy!
Best regards,
The JBC Team