Alice Ting @aliceyting Twitter profile | Pikagi

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Alice Ting

@aliceyting

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Molecular designer

Stanford, CA

https://t.co/AR0K0Q55lf

Joined June 2017

Don't wanna be here? Send us removal request.

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@aliceyting

Alice Ting

1 year

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase

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@aliceyting

Alice Ting

1 year

I was taken by surprise this morning when congratulatory emails started arriving in my inbox. I'm incredibly honored, grateful, and humbled to be included among such an esteemed group of scientists, many of whom I’ve admired for decades. While this recognition is individual,

@CarolynBertozzi

Carolyn Bertozzi

@CarolynBertozzi

1 year

A warm congratulations to my colleague and collaborator ⁦ @aliceyting ⁩ ⁦ @StanfordBiosci ⁩ on her election to the ⁦⁦ @theNASciences ⁩! 🎉 🎈 2023 NAS Election

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@aliceyting

Alice Ting

3 years

We wrote a proximity labeling (PL) review to highlight all the exciting applications of PL to protein-protein & protein-RNA/DNA interaction mapping. See examples of PL applied to enzyme-substrate interactions, insoluble baits, dynamic PPI networks & multifunctional proteins.

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@aliceyting

Alice Ting

2 years

Can we perform unbiased discovery of endogenous proteins that traffick from one organelle to another in living cells? Learn about our new proximity labeling method, TransitID, here: Full video of ASCB lecture at

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Alice Ting

5 years

Huimin Zhao shared this hilarious 1998 abstract on the “Directed Evolution of a Full Professor” at the #LSIsymposium2019 . “A pool of grad student clones was [selected] on minimal money media & the ability to work long hours w/vending machine snacks as a sole carbon source.”

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Alice Ting

3 months

Deeply saddened by the passing of Lubert Stryer, father of FRET, pioneer of DNA and peptide microarrays, author of the famous Biochemistry textbook used across the world. Many may not know that he was also an extremely generous supporter of the arts and deeply passionate about

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Alice Ting

1 year

Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but

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Alice Ting

3 years

First time giving a talk in a baseball stadium, with slides displayed on a jumbotron! At the Stanford Genetics retreat, where organizers have been creative & working hard to put on a safe in-person event *Retreat swag included baseball Tshirts and double helix baseball caps!

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Alice Ting

1 year

LOV-Turbo is published today in @naturemethods and plasmids for LOV-Turbo are available on Addgene, here: We hope many people will use LOV-Turbo in exciting new ways! If you plan to use NanoLuc BRET to activate LOV-Turbo, please

@aliceyting

Alice Ting

1 year

Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but

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Alice Ting

4 years

We’ve written a detailed step-by-step protocol for how to use TurboID (& split-TurboID) in cultured mammalian cells! How to select your proximity labeling (PL) enzyme, design & execute PL experiments, & analyze the resulting mass spec data.

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Alice Ting

3 years

To improve spatial and temporal control over proximity labeling, we’ve developed LOV-Turbo, a reversible & blue-light activated variant of TurboID. Almost no dark state leak & +light activity comparable to miniTurbo. We are doing pulse-chase labeling with LOV-Turbo to map..

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Alice Ting

1 year

RIP Christopher T Walsh, a giant of chemical biology. Today @CarolynBertozzi ’s Cancer Breakthroughs seminar at Stanford is dedicated to Walsh and the profound ways in which he influenced and inspired us all.

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Alice Ting

3 years

I learned about David Julius’ groundbreaking work from Roger Tsien, who commented that it was so exceedingly clever that he wish he had thought of it. I used to teach the TRPV1 paper at MIT to illustrate one of the most beautiful and impactful uses of Ca+2 indicators to…

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Alice Ting

2 years

Today’s incredible news is exciting on so many levels! As a lab, we use Click chemistry daily & it has transformed our research. When I was a grad student @CarolynBertozzi was the most supportive member of my @UCB_Chemistry committee and a rising female star I looked up to....

@NobelPrize

The Nobel Prize

2 years

BREAKING NEWS: The Royal Swedish Academy of Sciences has decided to award the 2022 #NobelPrize in Chemistry to Carolyn R. Bertozzi, Morten Meldal and K. Barry Sharpless “for the development of click chemistry and bioorthogonal chemistry.”

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Alice Ting

4 years

1/ Ca+2 indicators like GCaMP have been revolutionary for cell biology & neuroscience but a tool that gives stable genetic access to cells with transient Ca+2 activity (during user-specified time windows) could offer completely new capabilities - from large-scale imaging or ...

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Alice Ting

4 years

Split-TurboID out @ PNAS! Reconstitution across mito-ER contacts in HEK IDs 101 proteins, incl. 8 novel ones we validated by MAM purification or imaging. One of my favorite figures shows intracellular split-TbID reconstitution driven by cell-cell contact:

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Alice Ting

4 years

Want to try proximity labeling (PL) but don’t know how to get started? Sign up for our virtual workshop Nov 17-19! Hosted by Stanford Neuro-omics initiative. I will introduce the method and its applications, and my lab members will go through the nuts and bolts of designing &...

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Alice Ting

4 years

While many existing methods can ID the RNA binding partners of specific proteins, methods to do the reverse - ID protein partners of specific RNAs of interest - are lacking. We used both MS2 & dCas13 to target APEX to specific cellular RNAs for RNA-centered proximity labeling!

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Alice Ting

3 years

HiLITR is a molecular tool that converts complex phenotypes (protein localization & trafficking) into simple FACS readout. Combined w/CRISPRi screening, HiLITR enables HT discovery of genes that influence protein localization in various cell compartments.

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Alice Ting

5 years

"APEX-seq" for spatial transcriptomics in living cells, via APEX-catalyzed proximity labeling of RNAs. Beautiful collaboration with @HowardYChang , congrats to co-first authors @FurqanMFazal & Shuo Han. Insights into local translation of mito proteins.

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Alice Ting

3 years

Tickled that my 8-year old science-loving daughter gets to color me & my amazing female scientist colleagues in the “Think Like a Girl” coloring book from @Vilcek ! Amazing, creative & fun way to lift up #WomeninSTEM Happy Day of Women & Girls in Science!

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Alice Ting

7 months

Mark Howarth from the Univ. of Cambridge introduces “click biology”, simple and robust tools for non-linear covalent protein assembly. SpyTag and SpyCatcher were the earliest tools made from S. pyogenes adhesion protein that forms a spontaneous isopeptide bond bw Lys and Asp.

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Alice Ting

3 years

“photoTurbo” is TurboID caged at its catalytic K182 with UV-labile ONP. “chemTurbo" is caged at the same site with TCO, released by dimethyl-Tz in vivo. Both strategies give greater spatial & temporal control over prox labeling. from Peng Chen & Peng Zou:

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Alice Ting

4 years

Proximity labeling in yeast has been challenging. BioID has very low activity, while the APEX substrate biotin-phenol doesn't get in. Peng Zou provides a solution: alkyne-phenol, a yeast-permeable, clickable substrate for APEX that works well in yeast!

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Alice Ting

4 years

Excited to share our new molecular calcium integrator (“FLiCRE”) work just published in @CellCellPress ! After engineering & optimizing the calcium- & blue-light gating of FLiCRE, we use it in the mouse brain to map how 2 brain regions (PFC & NAc) are....

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Alice Ting

5 years

Directed evolution of “ultraTEV”, a faster variant of the biotechnological workhorse TEV protease, is out in Nature Methods. Tour de force by super-talented postdoc Mateo I Sanchez. Plasmids will be up on Addgene soon!

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Directed evolution improves the catalytic efficiency of TEV protease

Nature Methods - An optogenetic strategy enables selection of proteases with improved catalytic rates. The developed TEV protease variants are well suited for biotechnology applications, including...

@naturemethods

Nature Methods

5 years

Interested in developing or using better proteases? @aliceyting Alice Ting's lab has developed a platform for evolving proteases and used it to improve the catalytic efficiency of TEV protease.

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Alice Ting

1 year

Botulinum toxin (Botox) is best known as an anti-wrinkle treatment but the modular design of this bacterial protein has allowed it to be repurposed as a platform for delivery of therapeutically-useful protein and peptide cargoes into cells. Here we develop a highly sensitive and

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Alice Ting

9 months

Huge congratulations to Moungi Bawendi, our former collaborator and winner of the 2023 Nobel prize in Chemistry! Shortly after arriving at MIT I became inspired by the potential of quantum dots (QDs) for biological imaging with single molecule sensitivity and initiated an awesome

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Alice Ting

4 years

Video recordings + slides for our workshop on proximity labeling now posted: We had >1K registered attendees and tons of great questions during the live presentations. Thank you for the tremendous interest and for using PL methods in your research!

Proximity labeling workshop 2020 — Ting Laboratory

www.tinglab.org

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Alice Ting

4 months

LYTACs pioneered by @CarolynBertozzi opened the door to degradation of cell surface and extracellular proteins through lysosomal targeting. Here, we introduce genetically-encoded LYTACs, or GELYTACs, which are (1) easier to produce by recombinant expression (LYTACs, antibody

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Alice Ting

6 years

First experimental demonstration of protein identification with *single-molecule sensitivity*. A huge breakthrough from the labs of Edward Marcotte and Eric Anslyn @EricAnslyn

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Alice Ting

5 years

Roger Tsien was a visionary who came up with game-changing ideas decades before anyone else. This letter from 1982 envisions a “Calcium memory dye”. It only took 33 years for the field to catch up to Roger & finally produce CaMPARI, FLARE, etc. Hear about it @UCSanDiego tomorrow

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Alice Ting

4 years

Here is our foray into Cas13 engineering: to enable targeting of APEX to specific cellular RNAs, we fused a sequence-independent double-stranded RNA binding domain to dCas13d to improve stability of the dCas13-gRNA-targetRNA ternary complex. We then...

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Alice Ting

3 years

We are often asked whether protein X was detected by APEX or TurboID in Y location. To make it easy to search across all our published datasets, we compiled everything into Supplementary Tables 1 & 2 here. Search Table S1 by gene name, or search Table S2 by compartment/organelle.

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Alice Ting

4 years

Why does the heart beat faster during fight-or-flight response? This remarkable Nature paper performs APEX proximity labeling in beating mouse hearts (!!) to discover a new protein regulator (Rad) of voltage-gated Ca+2 channels. (1/2)

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Alice Ting

4 years

Instead of providing static proteome snapshots, proximity labeling can now be used to study protein *translocation*. TurboID is expressed in the ER lumen of source tissue, then biotinylated proteins are harvested by dissection from destination tissue.

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Alice Ting

5 years

Truly impressive seminar @Stanford by Matthias Mann @labs_mann , who has already revolutionized proteomics in so many ways. Now, he shows how close we are getting to single-cell proteomics: his newest instruments can detect 1000s of proteins from the equivalent of 4 HeLa cells!

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Alice Ting

3 years

The Ting lab is looking for postdocs interested in developing molecular technologies for recording, manipulating & discovering cellular behavior. We use state-of-the-art protein engineering, directed evolution, chemical synthesis, microscopy & proteomics. Email Alice to apply!

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Alice Ting

4 years

Split-TurboID enables conditional proximity labeling w/even greater spatial specificity than TurboID. We reconstituted split-TurboID across ER-mito contacts & enriched 101 human proteins, many of which have not prev. been linked to mito-ER contact sites.

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Split-TurboID enables contact-dependent proximity labeling in cells

Proximity labeling (PL) catalyzed by promiscuous enzymes such as TurboID have enabled the proteomic analysis of subcellular regions difficult or impossible to access by conventional fractionation-b...

www.biorxiv.org

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Alice Ting

4 months

We are recruiting! Open projects related to enzyme engineering, protein directed evolution, computational protein design, spatial proteomics, RNA tools, protein therapeutics, and molecular technology development for cell biology and neuroscience. We especially welcome trainees

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Alice Ting

2 years

"Sorry, Boston. Sorry, San Diego. The bay area is the center of chemical biology” - Jim Wells @ the 2022 QBI Chem Bio symposium @UCSF . :) It sure felt that way after spectacular talks today by @kevansf @christhechang @mbogyo @realJimWells @DanNomura @AlannaSchepartz Peter Kim...

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Alice Ting

2 years

Cre-inducible TurboID transgenic mouse used to map organ-specific secretory profiles and serum trafficking in vivo. Congrats Andrew McMahon, Rui Yang and team!

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A genetic model for in vivo proximity labelling of the mammalian secretome | Open Biology

Organ functions are highly specialized and interdependent. Secreted factors regulate organ development and mediate homeostasis through serum trafficking and inter-organ communication. Enzyme-cataly...

royalsocietypublishing.org

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Alice Ting

3 years

One of the great joys of being a PI is the international community of alumni that your lab creates. I was so lucky to be warmly welcomed by 3 former Thai students for my first visit to Thailand over New Years. We explored Bangkok, did a dinner cruise & checked out Chai’s new lab!

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Alice Ting

6 years

Too excited not to share - an EM image just taken by collaborators Daniela Boassa & Mark Ellisman, showing split APEX reconstituted across mito-ER contacts. Beautiful demo of split APEX for visualizing biology at high-res. Kudos to Yisu Han @lalostox for evolving split APEX!

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Alice Ting

5 years

Prime editing: a fundamentally new method for genome editing from @davidrliu . Guided by single “pegRNA” w/ both guide and donor, a reverse transcriptase directly writes the edit into the genomic locus. No DSB & enables edits not prev possible. Stunning!

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Alice Ting

4 years

Cell-type specific surface proteome mapping in the developing fly brain @Cell Terrific collab w/Liqun Luo. Co-1st dream team @JiefuBiol & Shuo Han Proximity labeling w/HRP & biotin-xx-phenol. 20 new protein regulators of synaptic wiring discovered.

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Alice Ting

4 years

From Fei Chen @broadinstitute : ticker tape technology uses ADAR-editable RNA barcodes to record the age of individual RNAs within living cells. Age of each RNA in population can subsequently be read out by scRNA-seq. Temporal resolution ~3hr. Super clever!

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Recording the age of RNA with deamination

Transcriptional programs implemented by cells often consist of complex temporal features, but current approaches to single-cell RNA sequencing only provide limited information about the dynamics of...

www.biorxiv.org

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Alice Ting

5 years

~6 yrs ago, super-res imaging by Xiaowei Zhuang’s lab revealed surprising, regularly-spaced actin rings along neuronal axons. Now, her lab makes progress figuring out what they are for: dynamically-regulated structural platforms for GPCR/CaM/RTK signaling

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Alice Ting

8 months

If your conference is named “OptoRevolution” it should have an impressive light display. Also this is the biggest display screen I’ve ever seen at a scientific meeting. Inspiring talks so far by Atsushi Miyawaki @jinzhanglab Robert Campbell @KarlDeisseroth Haifeng Ye and many

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Alice Ting

2 years

Stanford Biology is hiring faculty in biochemistry, cell & molecular biology! Check out the webinar: “Demystifying the application process for tenure-track faculty positions in the Biology Department at Stanford” Sept 1 Thurs, 10 am PT, open to all

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Alice Ting

4 years

In this new work, APEX proximity labeling is combined w/organic-aqueous phase separation (APEX-PS) to enrich xlinked RNA-protein complexes from specific subcellular regions. 100s of RNA binding proteins specific to nucleolus or outer mito membrane ID'ed.

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Alice Ting

3 years

"Functional proximity labeling (PL)" combines APEX with methods for enrichment of specific functional protein classes. We enrich compartment-specific phosphoproteins & O-GlcNAcylated proteins, and map novel RNA-binding proteins in the nucleus, nucleolus & outer mito membrane.

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Alice Ting

3 years

An IKEA-like webpage about phase separation! Complete with catalog of in vitro and in vivo building blocks … only missing meatballs and lingonberries brainchild of @BoeynaemsSteven in lab of Aaron Gitler at Stanford

www.designercondensates.org

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Alice Ting

1 year

Two mutations convert a natural fatty acyl-CoA synthetase from nonluminescent fruit fly into a luciferase (“FruitFire”) capable of generating light with the synthetic luciferin CycLuc2 in vivo (in the mouse brain). Not a de novo luciferase but shows how new functions can be

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Alice Ting

4 years

Happy Chinese New Year! 新年快乐 🎉🧨🐉🧧🐀 Our lab celebrated with a dumpling-making party! 🥟🥟🥟 (friends and colleagues in China: please stay healthy and safe!)

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Alice Ting

6 years

APEX-seq for RNA proximity labeling across 9 cell compartments produces RNA atlas of >3200 genes + many more transcript isoforms. Insights into local translation at mito. Exciting collab. w/ @HowardYChang and proud of co-first authors @F_M_Fazal & Shuo Han.

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Alice Ting

3 months

scFLARE leads to a change in clinical practice in humans? 🤯 When we made our calcium integrator scFLARE in 2020 we hoped that it would be used by basic neuroscientists to help dissect neural circuitry but we never imagined that it might lead to a change in clinical practice in

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Alice Ting

3 years

Stunningly simple protocol for highly-sensitive surface proteome mapping from @realJimWells : Add WGA-HRP (commercially-available, and binds with high affinity to surface glycans); label with biotin-phenol and H2O2 for 1 minute; then lyse/streptavidin beads/mass spec.

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Alice Ting

9 months

Interested in a faculty position at Stanford? If you are a creative scientist applying structural, computational, and/or biophysical approaches to biological problems, please apply for the Dept. of Structural Biology’s faculty search, deadline Nov 15. Structural biology is very

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Alice Ting

4 years

I am thoroughly enjoying Chris Walsh’s new textbook on the 13 human vitamins. Especially the chapters on my 2 most favorite vitamins, biotin and ascorbate. Highly recommended for anyone who loves metabolism and biosynthesis - a masterpiece of scholarship!

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Alice Ting

1 year

Directed evolution of enzyme activity in mammalian cells is hard. Here, @ReikaTei from @basktastic 's lab does a beautiful job evolving superPLD (phospholipase D) in HEK cells, improving catalytic efficiency up to 100-fold. SuperPLD is then used for acute light-dependent,

@ReikaTei

Reika Tei

1 year

Our work about superPLD is now published! In this paper, we describe how we can harness mammalian cell-based directed evolution to engineer a membrane editor (phospholipase D; PLD) with superior performance in mammalian cells. 1/n

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Alice Ting

5 years

Beautiful work by Michelle Chang in Nature: the bacterium S. cattleya naturally synthesizes a terminal alkyne-containing amino acid - useful as a functional group handle for Click chemistry. Congrats also to co-author and Ting lab alum Monica Neugebauer!

Discovery of a pathway for terminal-alkyne amino acid biosynthesis

Nature - Microbial generation of a terminal-alkyne-containing amino acid can be encoded into E. coli and provides the potential for in vivo generation of proteins and natural products for click...

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Alice Ting

1 year

We are recruiting postdocs! Open projects related to enzyme engineering, protein directed evolution, computational protein design, spatial proteomics, RNA tools, and molecular technology development for cell biology and neuroscience. We especially welcome talented postdoc

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Alice Ting

5 years

How do RNA granules move around in cells & neurons? By hitching a ride on lysosomes (via the adaptor protein ANXA11, discovered via APEX proximity labeling)! Amazing talk by Jennifer Lippincott-Schwartz at Stanford on work in review at Cell ()

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Alice Ting

6 years

Clever strategy for detecting & studying protein-protein interactions in the membranes of living cells. Immobilize bait protein with coverslip-tethered antibody, and do single molecule imaging of prey protein to see if it also stops moving.

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Direct visualization of single-molecule membrane protein interactions in living cells

Author summary Protein–protein interactions govern cellular processes. The majority of these physical interactions previously identified are strong/permanent interactions, which typically remain...

journals.plos.org

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Alice Ting

5 years

Our paper is out at eLife, showing that Luciferase can regulate the LOV domain via BRET. We used this to toggle bw light-control & drug (luciferin)-control of our SPARK tool. Congrats to the extraordinary @omgtinakim and co-authors Kelvin Cho and MW Kim!

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Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-pr...

SPARK2 allows a transcriptional readout of inter- and intracellular protein-protein interactions, with near-zero background, by employing proximity-dependent luciferase-LOV regulation.

elifesciences.org

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Alice Ting

3 years

Biotin cake to celebrate Kelvin Cho @kelvinfcho passing his thesis defense with flying colors!

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Alice Ting

5 years

An entire Methods in Molecular Biology issue on proximity labeling! Several APEX articles, and a Spot-BioID paper contributed by APEX co-inventor Hyun-Woo Rhee ().

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Alice Ting

3 years

PSA: when scheduling a committee meeting do not email your professors a doodle poll with 100 date/time options. narrow it down first (with your advisor, for example) then send a handful of options. or pick dates first, then times.

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Alice Ting

5 years

Impressive study from Anne-Claude Gingras using proximity labeling to map the human spatial proteome. 192 BioID fusion constructs across 32 subcellular compartments produce localization data for >4K proteins. Data at Preprint:

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A proximity biotinylation map of a human cell

Compartmentalization is an essential characteristic of eukaryotic cells, ensuring that cellular processes are partitioned to defined subcellular locations. High throughput microscopy[1][1] and...

www.biorxiv.org

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Alice Ting

4 years

Mouse secretome atlas using TurboID, from Jon Long's lab @Stanford ! TurboID expressed in the ER lumen of 4 different cell types, & biotinylated proteins enriched from plasma & analyzed by MS. Dynamic profiling +/- high fructose/sucrose diet as well.

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Cell type-selective secretome profiling in vivo

Secreted polypeptides are a fundamental biochemical axis of intercellular and endocrine communication. However, a global understanding of composition and dynamics of cellular secretomes in intact...

www.biorxiv.org

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Alice Ting

3 years

Lab retreat to Tahoe this year esp fun & rejuvenating after 2 retreat-less years. Snowshoeing, ziplining, cooking together, honing science elevator pitches, VR video games, s’mores, meandering science discussions & bonding. A few brave lab members even took a dip in frigid Lake..

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Alice Ting

7 months

I loved every minute of my first visit to South Korea, hosted by Hyun-Woo Rhee, the Rhee lab, and the SNU Chemistry Dept. Highlights included a palace tour while dressed in traditional hanboks, silly photobooth pics with the Rhee lab, magic tricks at a hipster cafe in Insadong,

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Alice Ting

5 years

TEV protease is widely used bc of its exquisite seq-specificity. We used directed evolution to improve its catalytic efficiency, employing light caging of TEV’s recognition seq to provide a kinetic selection. "ultraTEV" improves speed of FLARE & SPARK.

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Alice Ting

5 years

I tore my ACL playing tennis in Singapore over thksgiving & had ACL reconstruction surgery @Stanford 2 wk later. Spent holiday recuperating w/support of family. So grateful for modern medicine & w/renewed appreciation for body & health. Such a privilege to walk, run, play sports!

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Alice Ting

5 years

New work from Peng Zou et al. on light-activated proximity labeling of RNA (“CAP-seq”). Catalyst is miniSOG, which catalyzes RNA photooxidation that can be captured with propargyl amine and Click chemistry. New OMM biology. Congrats Zou & Wang labs!

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Alice Ting

4 years

Check out this terrific 6 minute talk on TurboID and its application in plants, by TurboID inventor @tessbranon , as part of the Plant Cell Atlas Workshop @Stanford ! Full length video at: under "Session 2"

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Alice Ting

5 years

Neuroscientists need ever-more specific methods to restrict expression of tools to defined neuronal subpopulations. This amazing study from @AllenInstitute uses scATAC-seq & scRNA-seq to discover enhancers that are even more specific than driver lines.

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Alice Ting

2 years

New preprint on a transcriptional reporter of cell-cell contacts (TRACC)! Trans-cellular binding bw peptide ligand & GPCR recruits arrestin-TEV for proteolytic release of TF, when blue light is present. We used TRACC to detect neuron-glioma contacts...

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A light-gated transcriptional recorder for detecting cell-cell contacts

Technologies for detecting cell-cell contacts are powerful tools for studying a wide range of biological processes, from neuronal signaling to cancer-immune interactions within the tumor microenvir...

www.biorxiv.org

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Alice Ting

4 years

On Mon Dec 7, Feng Zhang @zhangf & @tinakim_neuro will speak in Aaron Gitler's NeuroZoom series. Hear about Tina's Ca+2 integrator FLiCRE & its use in the brain to tag aversive circuits for re-activation & scRNA-seq (work in press @CellCellPress ). Join us!

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Alice Ting

6 months

“Hybrid incompatibility” occurs when the offspring of two closely related species have health problems or reduced reproductive capabilities, e.g. mules. The genetic basis of hybrid incompatibility has eluded scientists but new work from Molly Schumer shows that a clash between

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@aliceyting

Alice Ting

2 years

We are excited to welcome new postdoc @ShizhongD , whose PhD work with @kevansf just appeared in Cell! Shizhong and colleagues discovered cyclic peptides that specifically bind to either active (GTP-bound) or inactive (GDP-bound) forms of Galpha-s, and...

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@aliceyting

Alice Ting

4 years

This work presents our newest Ca+2 integrator, scFLARE, based on a designed Ca+2-activated TEV protease (CaTEV). We use CaTEV to catalyze intramolecular cleavage & release of a membrane-anchored TF, only in the presence of blue light. scFLARE is a ...

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Alice Ting

3 years

Exhilarating to speak to a live audience! Thank you @UCBerkeley SQB seminar series & my wonderful host @christhechang

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Alice Ting

5 years

Our paper on Directed evolution of split APEX is out at ACS Chemical Biology! Congratulations to @lalostox , @tessbranon , and Jeff Martell. Awesome collaboration with @ShechnerL and Mark Ellisman

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Directed Evolution of Split APEX2 Peroxidase

APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications from subcellular staining for electron microscopy to...

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Alice Ting

4 years

TurboID in live worms! The Feldman lab @Stanford uses TurboID in polarized epithelial cells to ID new proteins at ncMTOCs, follow up on 2 novel hits, and describe a model in which microtubule nucleation and anchoring are mediated by distinct complexes.

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Alice Ting

4 years

I am co-chairing the Mitochondrial Biochemistry Keystone mtg in Whistler in May. All-star lineup incl @VamsiMootha , Josh Rabinowitz, @chembioBryan , Cynthia Kenyon, @fiddle , Ben Tu, Tom Schwarz, Maya Shuldiner, @Pagliarini_Lab & many more! Students & post-docs encouraged 2 apply!

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Alice Ting

5 years

David Liu from Harvard/Broad gave a spectacular talk to Stanford Bioengineering today on his work developing powerful base editors. Did you know that the majority of pathogenic human genetic variations are point mutations? With @michaelzlin , 3 college classmates!

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Alice Ting

2 years

There are many COVID assays out there, but we tried to build one that is simpler to use and easier/cheaper to manufacture. Our S-BAT sensor is a single recombinant protein that can be expressed from lab strains of E. coli (or yeast), and detects spike antigen or cultured virus…

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Alice Ting

5 years

New study from Wade Harper using APEX proximity labeling to characterize the “mitochondria-autophagosome synapse” that forms upon mito depolarization, prior to mitophagy. Such comprehensive work, w/CRISPR-Cas9 screens also. ❤️ the schematics in Figs 4 & 6!

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Alice Ting

3 years

Backyard kick-off meeting for our neuro-omics phase 2 team @Stanford ! Great science, conversations, and food. Really, really missed in person science gatherings… @StephenQuake Liqun Luo @jure @alaurenoconnell Bo Wang @WeiQinChemBio

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Alice Ting

6 years

APEX-CLIP just reported by the lab of Markus Hafner in Nature Methods! Proximity labeling of endogenous proteins, followed by UV crosslinking to proximal RNAs. Analogous to APEX-RIP (), but higher spatial specificity & can map RBP occupancy patterns too.

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Alice Ting

5 years

This review on “Molecular tools for imaging and recording neuronal activity” was really fun to write with postdoc co-authors Wenjing Wang, a chemist, and Christina Kim, a neuroscientist.

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Molecular tools for imaging and recording neuronal activity

Nature Chemical Biology - A collection of genetically encoded tools, each with their own capabilities, limitations and performance characteristics, are available for monitoring and manipulating...

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Alice Ting

3 years

Our Neuro-omics initiative, started with a Big Ideas grant from @StanfordBrain , has been selected for Phase 2 funding! We are excited to go even bigger with transcriptomic, proteomic & connectomic technologies to study the brain, w/co-leaders Liqun Luo & @StephenQuake ...

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Alice Ting

4 years

Because peroxidases like HRP are often used for ultra-sensitive biomarker detection, we are attempting to repurpose some of our peroxidase reporters (e.g., APEX, split HRP) for COVID-19 detection. Thank you #fastgrants for supporting our efforts!

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@aliceyting

Alice Ting

5 years

So much fun jogging with @kaymtye in the lush San Diego canyons today, catching up on science and life. We first started running together at Stanford, then at MIT, then with our kids in strollers...

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Alice Ting

5 years

New funding opportunity from NIA/NIH just approved: use APEX and other related technologies to study brain proteome in aging and Alzheimer’s disease:

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Approved Concepts

Approved concepts are posted for interested researchers to plan projects.

www.nia.nih.gov

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Alice Ting

3 years

Korean BBQ dinner with the lab!

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Alice Ting

4 years

Work of Shuo Han and @Simenzhao and collaborators Sam Myers, Steve Carr, and Chuan He

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RNA-protein interaction mapping via MS2 or Cas13-based APEX targeting

RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. Capitalizing on...

www.biorxiv.org

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Alice Ting

2 years

Ken @kenleonloh of Loh et al. Cell 2016 (HRP-catalyzed prox labeling of synaptic cleft proteome) meets @JiefuBiol of Li et al. Cell 2021 (HRP-catalyzed prox labeling of neuronal surface proteome in vivo) for the first time! Magical moment at #ASBMBMassSpec @broadinstitute

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