Raj Gupta @Dr_RajatGupta Twitter profile | Pikagi

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Raj Gupta

@Dr_RajatGupta

1,558

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973

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Cardiologist at @harvardmed and @BrighamWomens . Genetics researcher @broadinstitute . Science at

Boston, MA

Joined January 2012

Don't wanna be here? Send us removal request.

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@Dr_RajatGupta

Raj Gupta

5 months

Our study is out @Nature . We identify non-lipid risk pathways for coronary artery disease using pooled CRISPR-interference for 2,285 genes at GWAS loci. With an amazing team we identified the genetic risk pathways for CAD that act in endothelial cells.

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@Dr_RajatGupta

Raj Gupta

5 years

Very excited to receive the New Innovator Award from the @NIHDirector . Recruiting scientists interested in the genetics of vascular biology. Check out our recent work and ongoing projects:

@NIH_CommonFund

NIH Common Fund

@NIH_CommonFund

5 years

The @NIHDirector ’s awards for High-Risk, High-Reward research have been announced for 2019 #NIHHighRisk #NIH

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@Dr_RajatGupta

Raj Gupta

1 year

I am not happy about the chosen name for something designed to replace me

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@Dr_RajatGupta

Raj Gupta

3 years

Post-docs and students! Join our fun team to connect vascular biology and genetics. Looking for post-doctoral fellows for the Gupta Lab. To apply:

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@Dr_RajatGupta

Raj Gupta

5 years

Excited to share our new study: Single cell analysis of the normal mouse aorta reveals functionally distinct endothelial cell populations. Now available online (1/7) @CircAHA

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Single-Cell Analysis of the Normal Mouse Aorta Reveals Functionally Distinct Endothelial Cell...

Background: The cells that form the arterial wall contribute to multiple vascular diseases. The extent of cellular heterogeneity within these populations has not been fully characterized. Recent...

www.ahajournals.org

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@Dr_RajatGupta

Raj Gupta

2 years

We just posted our latest work on the genetic mechanisms of heart disease in collaboration with @jengreitz 's lab @StanfordBASE on BioRxiv. … We used a Massive Scale Perturb-seq screen to identify the causal genes for Coronary Artery Disease 🧵

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@Dr_RajatGupta

Raj Gupta

6 years

Nice to see @TheOnion has picked up on the findings from @amitvkhera @skathire @connoremdin about genetics vs lifestyle!

@TheOnion

The Onion

6 years

Man’s Genetic Predisposition For Heart Disease No Match For 10 Half-Assed Push-Ups He Does Couple Times A Week

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@Dr_RajatGupta

Raj Gupta

5 years

Day 2 of @cziscience meeting. I am keeping a running tally of all the spatial RNA mapping technology acronyms! Did I miss any? Merfish Clamp-FISH IBEX Slide-seq HCR CODEX RNAscope STARmap Starfish MIBI @JCoolScience @DBBurkhardt @BrianBlackUCSF @anshulkundaje @Prof_Lundberg

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@Dr_RajatGupta

Raj Gupta

3 years

Thanks, Pradeep! This took a brutally long time, but we designed 2 functional assays for Corin, and tested all 64 missense variants from an exome sequencing study. The conclusions were not what we expected, but important nonetheless!

@pnatarajanmd

Pradeep Natarajan

3 years

Delighted to participate in this effort led by @wallacemwang @amitvkhera @Dr_RajatGupta elegantly using human and functional genomics to examine the relationship between CORIN and coronary artery disease @Circ_Gen

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@Dr_RajatGupta

Raj Gupta

6 years

Lunch with a Legend @broadinstitute . 1985 Nobel Prize Winner Joseph Goldstein kind enough to meet with trainees, and gave advice to have technical courage in pursuing important problems.

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@Dr_RajatGupta

Raj Gupta

2 years

Thank you Cornell Cardiology, @DrJamesLo and @WCMCardsFellows for hosting a very fun visit and Grand Rounds. I enjoyed talking about endothelial cells with an excellent group of vascular biologists and physician scientists!

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@Dr_RajatGupta

Raj Gupta

7 years

Hey @AnupamBJena , I'm the cardiology attending at MGH this week while everyone is at AHA meeting. Outcomes data this week is going skew even better than usual 😀

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@Dr_RajatGupta

Raj Gupta

1 year

Very enjoyable Variant-2-Function symposium @broadinstitute . Brilliant talks from @jengreitz @MelinaClaussnit @JamesTneal and @kasper_lage . Exciting prospects for high-throughput, unbiased experiments to link genetics to human disease.

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@Dr_RajatGupta

Raj Gupta

5 years

Wrapping up a beautiful week in Kyoto and Kobe, Japan for Endothelin-16. Learning old and new uses of inhibiting this fascinating pathway. Looking forward to #ET17 ! @DrKeeksPhD @davidpollock929

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@Dr_RajatGupta

Raj Gupta

5 years

Fun evening @BrighamWomens cardiology graduation. The roasts are much kinder than in my day. @CardioOncology @JoshuaBeckmanMD @TravisHinson @bwhcvls @APKithcartMDPhD

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@Dr_RajatGupta

Raj Gupta

3 years

Excited to give today's lecture on Lipid Therapeutics for the Harvard Biologic Chemistry and Molecular Pharmacology graduate students. I will find a way to work in my picture with Joseph Goldstein!

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@Dr_RajatGupta

Raj Gupta

6 years

I was a @SarnoffCardio Fellow in 2005-2006, and it has made an incredible impact on my career. I encourage all medical students interested in research apply. Happy to help! Read this introduction to the program in Circulation!

Sarnoff Cardiovascular Research Foundation | Circulation

www.ahajournals.org

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@Dr_RajatGupta

Raj Gupta

3 years

Excited to present @MedicalCollege Wisconsin on the genetic risk loci that affect vascular physiology tomorrow. Leaving lots of time for questions, when I hope the audience will redirect me from any misguided vascular physiology experiments! @ivorbenjamin2 @CardioOncology

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@Dr_RajatGupta

Raj Gupta

4 years

I got the BioNTech/Pfizer vaccine yesterday in the lobby of @BrighamWomens . Have a sore arm that is no big deal when I think about this miracle of modern medicine and science!

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@Dr_RajatGupta

Raj Gupta

2 years

So much fun to have the lab over for dinner. Thank you to this brilliant, dedicated group that showed great resilience through COVID. The best is yet to come! @VS_LeeKim Gavin, Shi, @MarkEPepin , Aure. @bwhcvls

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@Dr_RajatGupta

Raj Gupta

1 year

Really proud of the work presented by star postdoctoral fellow, Shi Fang, @broadinstitute MPG retreat. Summarizing our CRISPR screens to understand #vasculardisease .

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@Dr_RajatGupta

Raj Gupta

2 years

I am speaking at tomorrow's Presidential Session @expbio and @APSPhysiology on "The Endothelium and Integration of Physiology Responses" 9am/BallroomA. For interested #postdocs , my lab is #hiring for #fullyfundedpositions , so if you're interested I'd love to meet at the session!

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@Dr_RajatGupta

Raj Gupta

6 years

Spontaneous applause breaks out at #AHA when @DLBHATTMD read the wildly significant p-value for fish oil in Reduce-it trial.

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@Dr_RajatGupta

Raj Gupta

6 years

Congrats to the 2019-2021 @SarnoffCardio Scholars: @vnparikh and @AmyIsMyNickname ! Both working to improve our understanding of heart disease with genetics.

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@Dr_RajatGupta

Raj Gupta

5 years

Brilliant thesis defense @MIT by @askallur on “Defining endothelial cell heterogeneity”. His advisor, Elazer Edelman, described his brilliance quoting the Rig Veda. Congratulations Dr. Kalluri!! @harvardmed l

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@Dr_RajatGupta

Raj Gupta

2 years

I will be presenting this work at tomorrow's Latest Advances in Functional Genomics oral abstract session. #AHA22 3:30pm, Science and Technology Hall, Zone 1 Come with questions! Join our lab if you're interested!

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@kanghelenyihua

Helen Kang

@kanghelenyihua

2 years

Excited to share our latest work on understanding the genetics of heart disease using massive CRISPR screens and single-cell analysis: Thanks to our amazing team, especially co-first author Gavin Schnitzler, and @Dr_RajatGupta , @jengreitz Thread👇

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@Dr_RajatGupta

Raj Gupta

5 years

This story is so amazing. Scientists find mutation causing neurologic disease, AND create a drug that halts progression. Bedside-to-bench-to-bedside.

@NEJM

NEJM

5 years

Authors describe how molecular diagnosis of a rare, fatal neurodegenerative condition led to the rational design, testing, and manufacture of milasen, a splice-modulating antisense oligonucleotide drug tailored to a particular patient.

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@Dr_RajatGupta

Raj Gupta

3 years

Excited to work with this wonderful team of scientists to better understand non-coding gene regulation in cardiovascular disease

@jengreitz

Jesse Engreitz

3 years

Congratulations @kasper_lage and an amazing team in Denmark + US launching the Novo Nordisk Foundation Center for Genomic Mechanisms of Disease! @novonordiskfond @broadinstitute I am excited to work with this new center to map human gene regulation 👇 1/

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@Dr_RajatGupta

Raj Gupta

4 years

Interesting paper in @ESC_Journals that SNPs in 6p24 locus regulate Endothelin-1 levels in coronary microvascular dysfunction. Validates our prior work with @skathire @amitvkhera @jengreitz @BradEBernstein that minor allele associated with increased ET-1.

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Genetic dysregulation of endothelin-1 is implicated in coronary microvascular dysfunction

AbstractAims. Endothelin-1 (ET-1) is a potent vasoconstrictor peptide linked to vascular diseases through a common intronic gene enhancer [(rs9349379-G all

academic.oup.com

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@Dr_RajatGupta

Raj Gupta

4 years

Amazing tribute to the quintessential Cambridge dive bar- The Cantab Lounge in @nytimes . The floor was always weirdly sticky, and I didn't know Ben Affleck's dad was a bartender there.

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@Dr_RajatGupta

Raj Gupta

5 months

Endothelial cells are the first point of contact between cells and the blood stream, and mediate many functions related to CAD. Angiogenesis, inflammation, clotting, metabolism, and lipid transport are all EC functions relevant to heart disease.

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@Dr_RajatGupta

Raj Gupta

2 years

Loved this paper from @JChorba @gsmithMDPHD @pnatarajanmd . Such a well written example of a CRISPRi screen, with careful validation of the positive hits. Candidate genes are prioritized based on specificity and GWAS overlap.

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@Dr_RajatGupta

Raj Gupta

6 years

Proud to see my mentor @skathire present at the @uk_biobank conference. Historic significance of it being in the Mountbatten room is delightful

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@Dr_RajatGupta

Raj Gupta

1 year

It was a pleasure to work with such a talented group with creative approaches to understand mechanisms of inflammation driven by CHIP.

@ZhiYu_ACGT

Zhi Yu

1 year

Our new preprint "Human Plasma Proteomic Profile of Clonal Hematopoiesis" is out🎉! Mentored by @pnatarajanmd @Dr_RajatGupta , we leveraged paired DNA & proteomics data from ~62K humans and new scRNA-seq data from mice to uniquely characterize the proteomic landscape of CHIP🧬

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@Dr_RajatGupta

Raj Gupta

2 years

Great work from @SebastienRubin The CAD GWAS locus at chr6p24 is called the PHACTR1 locus. However my lab and others have shown an effect on Endothelin-1 expression. This paper shows NO vascular phenotype for PHACTR1 KO. My editorial summarizing the data:

@SebastienRubin

Sébastien Rubin

@SebastienRubin

2 years

Despite robust associations in GWAS with many vascular diseases, loss of PHACTR-1 function in the cells involved in vascular physiology does not appear to induce a pathological vascular phenotype in mice. Our work 👇👇 @insermU1034 @SBurtey @PLTharaux

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@Dr_RajatGupta

Raj Gupta

6 years

@hswapnil @BursatilBiotech @SpicyBrian @willsuh76 @ethanjweiss @skathire @MarkELindsay @EricSecemskyMD @ghoshhajra @APKithcartMDPhD @jpirruccello @pnatarajanmd @DrRosanneRouf @goosecoid I’m pretty sure my 3 year old daughter started this. 😂

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@Dr_RajatGupta

Raj Gupta

6 years

Before, during, and after a bike ride across the Golden Gate Bridge on a perfect day in San Francisco

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@Dr_RajatGupta

Raj Gupta

5 years

Congrats to @skathire on the new role. His passion for curing heart disease is inspiring, and I am sure he will succeed.

@skathire

Sek Kathiresan MD

5 years

News: After 22y @MassGeneralNews @broadinstitute understanding risk & resistance to heart attack, I am moving to new role as CEO @VerveTx - a biotech we have founded to develop gene editing Rx that permanently reduce risk of coronary disease in adults

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@Dr_RajatGupta

Raj Gupta

2 years

Congratulations, Vivian! It has been a pleasure to mentor this exceptional scientist and future colleague. Thanks for bravely joining my lab in the early days! More great science to come!!

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@Dr_RajatGupta

Raj Gupta

6 years

Public health and preventing death are absolutely in our “lane”.

@NRA

NRA

6 years

Someone should tell self-important anti-gun doctors to stay in their lane. Half of the articles in Annals of Internal Medicine are pushing for gun control. Most upsetting, however, the medical community seems to have consulted NO ONE but themselves.

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@Dr_RajatGupta

Raj Gupta

4 years

I was allowed to stay up late for 1988 Presidential Debate, and I still remember seeing two adults speaking intelligently. I thought about doing the same for my kids tonight...but glad I saved them from this disaster. The President is unpresidential.

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@Dr_RajatGupta

Raj Gupta

7 years

My 2 year old wanted "10 shirts" for her birthday. @UGP made it happen with short notice. Thanks @rishi_narayan_ !!

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@Dr_RajatGupta

Raj Gupta

6 years

Skiing in the clouds at Whistler for @KeystoneSymp #KSchromatin with @manoliskellis and @jengreitz

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@Dr_RajatGupta

Raj Gupta

5 years

I’ve enjoyed the podcasts from @runthelistpod . Favorite lesson so far: @NavinKumarMD would have been a cardiologist if we told more poop jokes

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@Dr_RajatGupta

Raj Gupta

7 years

My #BlackFriday shopping: @23andMe kit. Mostly excited for the raw data so @amitvkhera can calculate my polygenic risk scores. Ancestry won't be much of a surprise...I'm Indian.

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@Dr_RajatGupta

Raj Gupta

6 years

This paper shows a 6th vascular disease associated with the 6p24 locus--coronary artery dissection! Great work from @n_bouatianaji and colleagues. Also verifies our finding that the risk SNP regulates Endothelin-1 levels.

@n_bouatianaji

N Bouatia-Naji, PhD

6 years

Our article is now available @JACCJournals First genetic cause of #HeartAttack in Women #SCAD supported by @inserm @ERC_Research @FondationCoeur @sfcardio @beatscaduk

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@Dr_RajatGupta

Raj Gupta

6 years

Apartment Christmas tree versus house Christmas tree 🎄

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@Dr_RajatGupta

Raj Gupta

6 years

@skathire @VertexPharma @Regeneron @Amgen Interesting list! I'll add: G/D: beta myosin heavy chain/Hypertrophic CM Discovery: 1990 Medicine: Hopefully 2018 @MyoKardia Criticisms of GWAS that therapies haven't followed is very premature.

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@Dr_RajatGupta

Raj Gupta

2 years

I’d rather be able to dunk.

@edokeefe

Ed O'Keefe

2 years

“On average, short people live longer and have fewer incidences of cancer. One theory suggests this is the case because with fewer cells there is less likelihood that one goes wrong. I’d take that over dunking a basketball any day.”

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@Dr_RajatGupta

Raj Gupta

5 years

I read @EricTopol 's "Deep Medicine" this weekend.Expertly written and the span of his intellect is impressive. I was unexpectedly riveted by chapter on AI for personalized nutrition. Diet guidelines do seem haphazard, maybe the microbiome can explain inter-individual variability?

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@Dr_RajatGupta

Raj Gupta

4 years

Love this paper from @JonathanBrownMD and @davidrliu . They show a single injection of a base editor extends lifespan of Progeria mice from 215 to 510 days. Kuddos for also showing that AAV resulted in liver tumors. Great promise and some perils for this therapeutic approach.

@broadinstitute

Broad Institute

@broadinstitute

4 years

Researchers at Broad, @VanderbiltU , @NIH , and other institutions have used base editing in mice to treat progeria, a progressive and fatal genetic condition. The work, published in @Nature , provides a blueprint for addressing other genetic disorders.

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@Dr_RajatGupta

Raj Gupta

2 years

Lots of fun presenting at the @BWHGenetics retreat. Re-connecting with students, post-docs, and faculty after far too long!

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@Dr_RajatGupta

Raj Gupta

5 months

We also will study how the 41 endothelial cell-acting genetic variants affect CAD risk, and if patients with these variants have a different clinical presentation that can be used for prevention and treatment.

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@Dr_RajatGupta

Raj Gupta

7 years

It's an honor to be mentioned in an @skathire tweet.He discovered association, and this work builds on 10yrs of investigation in his lab

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@Dr_RajatGupta

Raj Gupta

5 years

Excited to be a part of the CZI Seed Network, working with an incredible group of scientists to define inter-individual variation with single cell analysis. @KArdlie @JD_Buenrostro

@cziscience

CZI Science

5 years

Collaboration drives progress in #science . Our new Seed Networks grants bring together 200+ scientists, engineers, and physicians to contribute to the @humancellatlas ! Learn more: #HumanCellAtlas

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@Dr_RajatGupta

Raj Gupta

5 years

@sacjai Rejection for medication approval: Patient needs to have the disease (heart attack) before access to preventive medicine (PCSK9i) is granted.

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@Dr_RajatGupta

Raj Gupta

5 years

The most interesting heterogeneity was in the 3 subpopulations of Endothelial Cells. EC1: Increased expression of Vcam1, adhesion/matrix genes EC2: Lipid handling, angiogenesis genes EC3: Markers of lymphatic ECs (3/7)

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@Dr_RajatGupta

Raj Gupta

5 months

TLNRD1 is a new regulator of endothelial cell function, which was not previously known. It has the same effects as CCM2 on KLF2 expression and developmental phenotypes in zebrafish.

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@Dr_RajatGupta

Raj Gupta

6 years

Wow! It sounded like snake oil, but REDUCE-IT trial shows fish oil can reduce risk by 25%.

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@Dr_RajatGupta

Raj Gupta

5 years

Kicking off the @cziscience Seed Network meeting with an introduction by @betenoire1

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@Dr_RajatGupta

Raj Gupta

8 years

So happy to see I got @NIH_LRP funding! Great program for early career physician-scientists.

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@Dr_RajatGupta

Raj Gupta

7 years

The power of @uk_biobank demonstrated in excellent paper from @niek_verweij . Happy the 6p24 locus is referred to as EDN1 and not PHACTR1in Table 2 😀

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@Dr_RajatGupta

Raj Gupta

5 months

The top two genes with regulatory effects on CAD-associated genetic programs were CCM2 and TLNRD1. Unexpectedly, we found that they bind each other, and both regulate the response of endothelial cells to blood flow.

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@Dr_RajatGupta

Raj Gupta

4 years

Excited to hear @nevillesanjana present this outstanding work at @BWHGenetics today. We were post-docs @broadinstitute together, and it is always great to hear the inventor of genome-wide CRISPR screens spin knowledge!

@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

As promised, here’s an overview of our study to find host factors required for SARS-CoV-2 infection in human cells. We use a genome-wide CRISPR screen to identify genes (& key mechanisms) that might be therapeutically targetable to prevent viral infection.

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@Dr_RajatGupta

Raj Gupta

7 years

How 1 non-coding variant influences risk for 5 vascular diseases. . Nice summary at:

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@Dr_RajatGupta

Raj Gupta

5 months

Here we developed a new analytic approach that combines genetics and functional characterization (the “Variant-to-Gene-to-Program (V2G2P) pipeline”), and applied it to study the role of endothelial cells in CAD. This is expertly summarized by @jengreitz and @kanghelenyihua

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@Dr_RajatGupta

Raj Gupta

5 years

This was a really fun collaboration. Thank you to the excellent first authors of this paper ( @askallur and @kvshamz ), our collaborators in the Edelman Lab, Regev Lab, and the Broad-Bayer precision cardiology lab ( @patrick_ellinor @broadinstitute , @skathire ) (7/7)

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@Dr_RajatGupta

Raj Gupta

7 years

@skathire @broadinstitute @BradEBernstein @MGH_RI @MassGeneralNews @MGHMedicine @MGHHeartHealth We were very motivated to study this mutation because of the effect on 5 seemingly unrelated vascular diseases.

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@Dr_RajatGupta

Raj Gupta

1 year

Great seminar on Tuesday, March 14. @BWHGenetics is hosting Doug Marchuk, a leader in the field of vascular genetics. He has uncovered the causes of many rare vascular disorders, including Cerebral Cavernous Malformations. Boston-area #trainees can contact me to attend the lunch

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@Dr_RajatGupta

Raj Gupta

6 years

Love how @AnupamBJena can use data to see how we make decisions! I did not know "Judges are less likely to grant asylum when their alma mater has unexpectedly lost a football game" !

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@Dr_RajatGupta

Raj Gupta

3 years

This was such a great idea @PardisSabeti and @Vilcek ! My girls spent the day coloring these pictures and teaching me about amazing women in science like Ruth Lehman @WhiteheadInst

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@PardisSabeti

Pardis Sabeti

3 years

On this International Day of Women and Girls in Science, we honor all those who paved a path for #DiversityinSTEM . I am grateful to @Vilcek for their tireless work in this space and proud to be included in their coloring book celebrating #WomeninSTEM

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@Dr_RajatGupta

Raj Gupta

5 years

Highly recommended Thanksgiving reading. Beautiful memoir by my friend @aarti411 , an NPR reporter telling the story of her family figuring out life in America.

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@Dr_RajatGupta

Raj Gupta

7 years

This is me today.

@legogradstudent

Lego Grad Student

@legogradstudent

7 years

Packing numerous books and papers that he plans to read over winter break, the grad student deludes himself.

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@Dr_RajatGupta

Raj Gupta

6 years

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@Dr_RajatGupta

Raj Gupta

5 years

Inspiring research to identify the viral cause of Acute Flaccid Myelitis. Collaboration between @michaelneuro and Riley Bove, two intern year classmates of mine @MGHMedicine . It was clear even then that they would both go on to accomplish amazing things!

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What Causes a Mysterious Paralysis in Children? Researchers Find Viral Clues (Published 2019)

In one study, nearly 70 percent of children with the disease had antibodies to common enteroviruses that usually produce mild effects but can sometimes cause neurological problems.

www.nytimes.com

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@Dr_RajatGupta

Raj Gupta

1 year

@pnatarajanmd @atvbahajournals Interesting paper. Confusing that the direction of effect is opposite of genetics (reduced PHACTR1 expression is associated with CAD risk in GWAS/eQTL). Also, the PPARg effect is unexpected because the 6p24 locus is not associated with LDL/Tgs. GWAS biology is always complicated!

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@Dr_RajatGupta

Raj Gupta

6 years

Every great team needs someone to hold the bag of hot Cheetos.

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@Dr_RajatGupta

Raj Gupta

6 years

All disease associations may be explained by vascular and hemodynamic effects of ET-1, though there is more to learn about this fascinating vasoactive peptide.

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@Dr_RajatGupta

Raj Gupta

3 years

I will also get to mention my favorite @NEJM article of all time. "Normal plasma cholesterol in an 88-year-old man who eats 25 eggs a day". Amazing paper:

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@Dr_RajatGupta

Raj Gupta

7 years

@ethanjweiss Haha! Yes, and it's so amazing I've been told it will win 1-3 Nobels. 🙃

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@Dr_RajatGupta

Raj Gupta

3 years

@anshulkundaje Lyme is common enough in your area that I recommend you get tick tested. Risk of transmission pretty low in only 3 hours, but if tick is positive I’d start prophylactic doxycycline. @roby_bhatt ?

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@Dr_RajatGupta

Raj Gupta

4 years

Exceptional review of Spontaneous Coronary Artery Dissection (SCAD) by @EstherSHKimMD . Great discussion of management, genetics, and gaps in our understanding of this disease. Thanks for referring to the GWAS risk locus as the PHACTR1-EDN1 locus!

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Spontaneous Coronary-Artery Dissection | NEJM

Coronary-artery dissections account for less than 1% of acute myocardial infarctions, occur most commonly in women and most often between the ages of 47 and 53 years, may be associated with an unde...

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@Dr_RajatGupta

Raj Gupta

5 months

Thanks to a wonderful team! @jengreitz @kanghelenyihua Gavin R. Schnitzler, Shi Fang, Ram Angom, Vivian Lee-Kim, @xRosaMa @tkyzeng @KrishnaAragam Marty Taylor. @bwhcvls @broadinstitute @MGBInnovation

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@Dr_RajatGupta

Raj Gupta

1 year

Here's a new one: Ordered imaging test. Pt's insurance requires prior authorization, so I need to justify to an insurance doc. BUT the insurer is also MY HOSPITAL: Mass General/Brigham! So to order a test, my own hospital system needs me to get approval from another doc! Insane!

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@Dr_RajatGupta

Raj Gupta

5 months

No medications directly target endothelial cells for the prevention or treatment of CAD, so we looked to genomewide association studies to identify new clues for causal mechanisms.

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@Dr_RajatGupta

Raj Gupta

1 year

@KathrynMooreLab @GordonConf @AWeinstock @yvancharvet @SwirskiLab @Yale @mallat_lab @AJMurph5 @mhlangalab @WillemNANO @IntMedAbel Incredible meeting! Wonderful science, people, and views of the Mediterranean. Hasta luego, Barcelona.

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@Dr_RajatGupta

Raj Gupta

7 years

Nice summary of our recent @CellCellPress paper (with @skathire ) .

@NatureRevGenet

Nature Reviews Genetics

@NatureRevGenet

7 years

COMPLEX DISEASE: From non-coding risk variant to biological mechanism in CAD #researchhighlight by @LinkoNRG

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@Dr_RajatGupta

Raj Gupta

6 years

Beautiful paper from the Rajagopal lab on single cell RNA-seq for airway epithelial cells. Pulse-seq experiments are interesting. Can anyone explain what a Krt5-creER/LSL-mT/mG mouse is? How does it label all basal cell progeny? @goosecoid

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A revised airway epithelial hierarchy includes CFTR-expressing ionocytes

Nature - Single-cell RNA sequencing analysis identifies cell types and lineages in airway epithelium, including the pulmonary ionocyte, a new cell type predominantly expressing the cystic fibrosis...

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@Dr_RajatGupta

Raj Gupta

5 months

We found 41 CAD-associated variants that regulate CAD-associated genetic programs in our massive-scale Perturb-seq. To get here, we knocked down expression of 2,285 genes with 37,637 CRISPR guide RNAs and sequenced 214,000 individual endothelial cells. 💪 work by Gavin Schnitzler

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@Dr_RajatGupta

Raj Gupta

3 years

Congrats @michellemorse ! So well deserved. Always knew you were a leader from when we met as roommates in Botswana as @PennMedicine students!

@michellemorse

michellemorse

3 years

Its official! Today is my first day as New York City's Deputy Commissioner for the Center for Health Equity + Community Wellness + first-ever Chief Medical Officer of the Department of Health + Mental Hygiene! Lets go! Honored to work w/ @NYCHealthCommr @DrTorian + all teams 1/3

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@Dr_RajatGupta

Raj Gupta

6 years

Sure, I'm impressed by these results from the Odyssey trial. But I'd be more impressed if they could figure out a price for these darn meds that would allow patients to get them without 1 year of paperwork!

@CMichaelGibson

C. Michael Gibson MD

@CMichaelGibson

6 years

Significant reduction in primary endpoint of MACE in Odyssey with PCSK9 inhibitor alirocumab

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@Dr_RajatGupta

Raj Gupta

5 years

Nice to see paper by @IAmDrIbrahim and @JJheart_doc referenced often at Endothelin-16 meeting in Kobe, Japan. Clinical implications of high ET-1 in CHF and coronary disease still need to be worked out.

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@Dr_RajatGupta

Raj Gupta

4 years

@jpirruccello My ophtho wife says subconjuctival hematoma...from coughing...from COVID! See, we still got there

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@Dr_RajatGupta

Raj Gupta

7 years

Got my @23andMe Ancestry report. Somehow I'm 0.1% Finnish?!?! Is this real? Some error in calling southeast asian haplotypes? @sabeti_lab @manuelrivascruz @amitvkhera @tuuliel

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@Dr_RajatGupta

Raj Gupta

5 years

We asked a simple question, what are the different cells that make up the aorta? Using single cell sequencing we analyzed over 6000 cells from 4 mice. With gene expression data from 1900 genes/cell we identified 10 cell types in the Aorta. (2/7)

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@Dr_RajatGupta

Raj Gupta

7 years

Quick intro to fascinating @CellCellPress paper from @erezaterez . 3D DNA loops are fascinating

@NewsfromScience

News from Science

@NewsfromScience

7 years

Watch the human genome fold itself in four dimensions:

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@Dr_RajatGupta

Raj Gupta

5 months

This study establishes a high-throughput pipeline for functional characterization of disease-associated genetic variants. We look forward to applying this to the thousands of variants for which a functional mechanism is not known.

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@Dr_RajatGupta

Raj Gupta

5 years

@bwhcvls @BrighamFellows @skathire @VerveTx @MGHHeartHealth @broadinstitute @tmmichelangelo Ha! Thanks to all for coming. Official title was: The hope and hype of crispr editing.

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@Dr_RajatGupta

Raj Gupta

7 years

Nice summary of clonal hematopoiesis. Is all clonal expansion bad, or does it matter which clone? @jaiswalmdphd @pnatarajanmd

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@Dr_RajatGupta

Raj Gupta

4 years

@skathire I'm a Level 10 Ikea assembly ninja. Just say the word, and I'll have it assembled by dinner.

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