Neville Sanjana @nevillesanjana Twitter profile | Pikagi

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Neville Sanjana

@nevillesanjana

4,784

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New York, NY

https://t.co/GOM83VXkMr

Joined June 2010

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 months

🚨New lab preprint! 🚨 A deep dive to shed light on a long-standing controversy with RNA-targeting CRISPRs: Do they have high levels of collateral (trans) cleavage of other RNAs in the cell ✂️✂️✂️ — or not? New work from talented PhD student #SydneyHart & others in our lab. 📰

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

As promised, here’s an overview of our study to find host factors required for SARS-CoV-2 infection in human cells. We use a genome-wide CRISPR screen to identify genes (& key mechanisms) that might be therapeutically targetable to prevent viral infection.

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

A TWEETORIAL on our new CRISPR-sciATAC preprint. Our lab has been working on combining pooled CRISPR screens with single-cell chromatin accessibility for nearly 4 years so very happy to see this work finally out there in the world.

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Scalable pooled CRISPR screens with single-cell chromatin accessibility profiling

Pooled CRISPR screens have been used to identify genes responsible for specific phenotypes and diseases, and, more recently, to connect genetic perturbations with multi-dimensional gene expression...

www.biorxiv.org

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@nevillesanjana

Neville Sanjana

@nevillesanjana

9 months

TWEETORIAL time! In new work, we identify required transcription factors for neural differentiation using a TFome-wide CRISPR knock-out library. Congrats to first authors @congyiliv and @chaodai (from our lab) and @ggaripler (from @estebanmazzoni lab). 👏👏👏

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

Delighted to present CIRCLE! 🔴🟠🟡🔵🔵🟣🟤⭕️🔄⏺️⚫️ An approach to use tumor exomes to predict immunotherapy outcomes. That is, cancer patient responses in the clinic.

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

This semester I've had a lot of fun teaching an advanced undergrad seminar on genome engineering and CRISPR. 🧬✂️⚙️💉 In case anyone else is putting together a similar class, topics covered & reading list are available here: Feel free to reuse! ♻️👍

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Excited to share our recent work: Measuring how different chromatin modifying enzymes impact chromatin accessibility genome-wide with single-cell resolution. Led by @noaliscovitch & @antonino_nyc , CRISPR-sciATAC includes contributions from many lab members & colleagues. 👏👩‍🔬👨‍🔬

@NatureBiotech

Nature Biotechnology

3 years

Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

Our lab's recent work on harnessing RNA-targeting CRISPRs for forward transcriptomic screens in human cells is now published @NatureBiotech . We hope this platform will be of broad utility for geneticists and biologists with applications for diverse phenotypes and diseases.

@NatureBiotech

Nature Biotechnology

4 years

Massively parallel Cas13 screens reveal principles for guide RNA design

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

New work from our lab: How can we program T-cells to make better immunotherapies? ⌨️💻🦠 Take a tour of the paper in this fantastic thread from first author (and multi-disciplinary innovator) @MatLegut ⤵️⤵️⤵️

@MatLegut

Mat Legut, PhD

2 years

Really chuffed to share our latest work from Sanjana Lab out in @nature today (). We tested >12,000 genes to find positive regulators of T cell proliferation to be used for next-gen #immunotherapies . A thread...

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@nevillesanjana

Neville Sanjana

@nevillesanjana

9 months

Happy 10th birthday to CRISPR pooled screens! 🎂🎂🎂🎂🎂🎂🎂🎂🎂🎂

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@nevillesanjana

Neville Sanjana

@nevillesanjana

11 months

TWEETORIAL on our new paper characterizing loss of the autism-associated gene CHD8 using isogenic CRISPR-edited stem cells and neurons! ✂️🧬🧠 Link to paper:

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Heterozygous deletion of the autism-associated gene CHD8 impairs synaptic function through widesp...

This study investigates the impact of CHD8 mutations on autism spectrum disorder (ASD). Using engineered heterozygous CHD8 human stem cells, we found alterations in gene expression, chromatin...

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@nevillesanjana

Neville Sanjana

@nevillesanjana

7 years

Happy to announce that our pooled CRISPR screen design tool (GUIDES) is now published:

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

This is so cool. A CRISPR screen discovers a new synthetic lethality that is druggable. Then, a small-scale clinical trial shows proof-of-concept in previously treatment-resistant folks, identifying a new therapy for ~50% of liver cancers. Incredibly impressive work!

@mvicaracal

Victoria Aranda

3 years

Online at @nature : EGFR activation limits the response of liver cancer to lenvatinib by Rene Bernards and colleagues #cancer #clinicalresearch

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

Thank you @NEJM (& Andrea @SchietingerLab ) for profiling our lab's recent work in Clinical Implications of Basic Research: We are thrilled by the possibility of bringing modifier-enhanced T cell therapies to the clinic!

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

Happy to share our new preprint characterizing a recent mutation in SARS-CoV-2 Spike protein (D614G) and its potential functional impact on the ability of the virus to transduce human cells.

@biorxiv_genetic

bioRxiv Genetics

@biorxiv_genetic

4 years

The D614G mutation in SARS-CoV-2 Spike increases transduction of multiple human cell types #biorxiv_genetic

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@nevillesanjana

Neville Sanjana

@nevillesanjana

5 years

TWEETORIAL on our new preprint: A genome engineering shootout (!) between wild-type Cas9 and 2 different PAM-flexible CRISPR enzymes — xCas9 and Cas9-NG — across 3 different tasks: knock-out, CRISPRi, and CRISPRa (1/15)

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

New review article from @lpinello & colleagues in @MolecularCell : Technologies and Computational Analysis Strategies for CRISPR Applications

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@nevillesanjana

Neville Sanjana

@nevillesanjana

7 years

Very cool — anti-mouse/rabbit secondary antibodies (nanobodies) produced in E. coli instead of animals.

@Addgene

Addgene

7 years

New plasmids lab of Dirk Görlich: A toolbox of anti–mouse and anti–rabbit IgG secondary #nanobodies

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Happy to announce that our Prime Editing Design ✏️ tool () has now been published: "Automated design of CRISPR prime editors for 56,000 human pathogenic variants"

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Automated design of CRISPR prime editors for 56,000 human pathogenic variants

Biotechnology; Computational bioinformatics

@johnomix

John Morris

4 years

Excited to share this preprint from the @nevillesanjana lab, led by myself and an ultratalented NYU undergrad, Jahan Rahman ( @jahanpehechanho ). We designed CRISPR prime editing reagents to target thousands of human pathogenic variants. Key findings 1st! 👇

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

If this is true.... mind blown! RT-qPCR without the need for a separate RT enzyme (i.e. just Taq). Will be great to see if this is robust across many applications/templates.

@biorxivpreprint

bioRxiv

@biorxivpreprint

4 years

One enzyme reverse transcription qPCR using Taq DNA polymerase #bioRxiv

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@nevillesanjana

Neville Sanjana

@nevillesanjana

1 year

Happy to share the latest work from our group & @tuuliel (led by the stellar @johnomix ) — STING-seq & beeSTING-seq! See John's explainer 👇 about why we think these approaches will help with variant-to-function challenges in human genetics (e.g. association ➡️ causation).

@johnomix

John Morris

1 year

🚨Our work on multi-modal, single-cell pooled CRISPR screens to study GWAS variant-to-function, STING-seq, is now out in @ScienceMagazine ! Read on for 6⃣ of the main findings from our study, led by myself and co-advised by @nevillesanjana and @tuuliel ! 🎉

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

Orgo-seq: Neat paper on ASD driver genes combining single-cell and bulk RNA-seq. Also: 1,420 cerebral organoids (!!) from 25 donors.... literally 🤯 by so many🧠🧠🧠! From @elimtt , @geochurch , @TalkowskiLab & team. Looks like an awesome dataset! 👍

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

Amazing.... from CRISPR screen (2015) to sickle-cell cure. The age of gene & cell therapy marches on! 🙌

@skathire

Sek Kathiresan MD

2 years

Wow, say it with me: “a functional cure” for sickle cell disease with CRISPR gene editing All 31 patients with severe SCD, no vaso-occlusive crises (f/u 2 to 32 mos) Similar durability and efficacy for beta-thal 👏🏽👏🏽👏🏽 @CRISPRTX @VertexPharma

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@nevillesanjana

Neville Sanjana

@nevillesanjana

8 years

Happy to report that our noncoding CRISPR screen work is out today in @sciencemagazine : Congrats to the whole team!

@nevillesanjana

Neville Sanjana

@nevillesanjana

8 years

Big day! We also have a new paper on CRISPR for noncoding functional genomic screens on @biorxivpreprint :

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Neville Sanjana

@nevillesanjana

4 years

New preprint from our group for the automated design of CRISPR-based prime editors to target thousands of human pathogenic variants in ClinVar:

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Automated design of CRISPR prime editors for thousands of human pathogenic variants

Prime editors (PEs) are CRISPR-based genome engineering tools that can introduce precise base-pair edits at specific locations in the genome. These programmable gene editors have been predicted to...

www.biorxiv.org

@johnomix

John Morris

4 years

Excited to share this preprint from the @nevillesanjana lab, led by myself and an ultratalented NYU undergrad, Jahan Rahman ( @jahanpehechanho ). We designed CRISPR prime editing reagents to target thousands of human pathogenic variants. Key findings 1st! 👇

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Delighted to see this work from our lab is now published as the first-ever (!) article in @CellGenomics : Congrats to PhD student @CathyxGuo & many other lab members. Check out our previous tweetorial for an overview 👇

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Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

To accelerate CRISPR-based targeting of RNA, Guo et al. present a resource with optimized RfxCas13d guide RNAs (gRNAs) to target messenger RNAs and noncoding RNAs in six common model organisms and...

@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

New preprint from our group: “Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens” led by @CathyxGuo with big help from @we_harm , @alexmenman and #DanHaro .

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Exciting study: Caffeine improves cardiovascular outcomes via reduction of PCSK9, an established regulator of cholesterol & heart disease. Further justification for a daily coffee/tea habit. ☕️ (not that anyone needed further justification...)

@pnatarajanmd

Pradeep Natarajan

3 years

Comprehensive investigation in cells, mice, and humans shows how caffeine reduces the expression & secretion of PCSK9 @NatureComms

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@nevillesanjana

Neville Sanjana

@nevillesanjana

7 years

This is an amazing resource for R/RStudio, Python, NGS analysis, stats, functional genomics, and more. Thank you for this nice resource!

@rafalab

Rafael Irizarry

7 years

We've updated our page to include 75 videos from our #Python for Research course by @jponnela

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Neville Sanjana

@nevillesanjana

3 years

Happy to share new work from a collaboration with our lab neighbor ( @satijalab ) that combines multiplexed Cas13 RNA perturbations with single-cell multiomics! Congrats to postdocs @harm__w & @alexmenman who led the work 👏 A writeup of highlights from the preprint from Harm👇

@harm__w

harm

3 years

Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic interrogation of gene function and regulatory networks. And we have become quite efficient at profiling single gene perturbations at a jaw-dropping scale ( @josephmreplogle , @JswLab ).

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@nevillesanjana

Neville Sanjana

@nevillesanjana

5 years

Honored to be among the #PECASE awardees. Thank you to the US science funders that have supported our lab's work, including @NIH @genome_gov @theNCI @DARPA . Grateful for the support & looking forward to more great science in the future.

@genome_gov

National Human Genome Research Institute

5 years

Congratulations to #NHGRI funded #PECASE awardees (left to right) Neville Sanjana @nevillesanjana , Adam Phillippy @aphillippy and David Zhang on their exceptional contributions to the field of genomics!

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@nevillesanjana

Neville Sanjana

@nevillesanjana

5 years

Our new preprint on harnessing CRISPR-Cas13 for forward transcriptomic screens in human cells is online.

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Principles for rational Cas13d guide design

Type VI CRISPR enzymes have recently been identified as programmable RNA-guided, RNA-targeting Cas proteins with nuclease activity that allow for specific and robust target gene knock-down without...

www.biorxiv.org

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

Happy to share this work with the world. This was really a fantastic collaboration between @virusninja and #TristanJordan and @ZDaniloski in my group! 🙌 Full tweetorial about the study coming soon... 🙂

@virusninja

Benjamin tenOever

4 years

Do you want to know how #SARSCoV2 enters and takes over our cells? Check out the most recent work from @nevillesanjana and my own group describing a whole genome #CRISPR screen. Congrats to first authors @ZDaniloski & Tristan Jordan

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@nevillesanjana

Neville Sanjana

@nevillesanjana

1 year

Delighted to share our lab's newest work to use RNA-targeting CRISPRs to tune and modulate gene expression! ✂️🎛️ ➡️ ➡️ ➡️ 🔇🔉🔊 Check out the superb TWEETORIAL from former postdoc @we_harm below👇

@harm__w

harm

1 year

Excited to share our new work on RNA-targeting CRISPRs. Our team ( @AndrewStirn , @david_a_knowles , @nevillesanjana & myself) developed TIGER a deep learning model that can be used to precisely control/titer gene expression!

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@nevillesanjana

Neville Sanjana

@nevillesanjana

2 years

Not gonna lie: I will be using this at bedtime tonight. 📜✂️🧬💤

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@nevillesanjana

Neville Sanjana

@nevillesanjana

7 years

We ( @tuuliel and I) are launching the Genomic Innovation course this Fall. @nygenome @nyuniversity @Columbia

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@nevillesanjana

Neville Sanjana

@nevillesanjana

5 years

Thrilled to see our multi-CRISPR pooled screen for CRISPR-KO, CRISPRi, and CRISPRa is featured on the cover of @CellReports (artwork by @CathyxGuo ). Congrats to Mat Legut and Zharko Daniloski who led this work!

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

So many applications of CRISPR in cancer biology! 🧬🧫✂️ Had a blast working on this review with @alynakatti & @biancaaadiaz in @dow_lab (Bianca is a former MS student from our group, now in Dow Lab) & postdoc @cmcaragine from our group. Congrats to the whole team! 👏

@NatureRevCancer

Nature Reviews Cancer

@NatureRevCancer

3 years

NEW content online! CRISPR in cancer biology and therapy

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Neville Sanjana

@nevillesanjana

2 years

Great overview of gene editing and gene therapy approaches marching toward the clinic. Also some great insights about viral vs. non-viral delivery:

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For hemophilia and thalassemia, a new era of ‘one-and-done’ gene therapies has arrived

Nature Biotechnology - Industry celebrates two newly approved genetic medicines, but patients with these blood disorders may face a long wait, and many will never access them because of their high...

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Happy to share that our study to develop chemically-modified guide RNAs & RNPs for CRISPR-Cas13 in human cells (including primary cells) is out today: Major congrats to co-first authors (& Cas13 super team in our lab) @alexmenman & @we_harm .

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Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

By screening multiple chemical modifications of the CRISPR RNA (crRNA), Méndez-Mancilla et al. identify specific chemical modifications and placement of modified bases that improve CRISPR-Cas13...

@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Preprint alert! 🚨 New work from our group for transcriptome engineering in human cells — including in T cells & to target SARS-CoV-2 — using chemically-modified guides and Cas13 RNPs. Postdocs @alexmenman & @we_harm led the work & here's a great summary from Alex: 👇👇👇

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Neville Sanjana

@nevillesanjana

7 years

Amazing.... I remember taking CS229 17 years ago (!) and it was a small class. Maybe 30 people tops. Great class with @chrisbregler .

@AndrewYNg

Andrew Ng

7 years

Stanford's first day of class--record-breaking 1040 people already enrolled for on-campus Machine Learning (CS229). Wow! @danboneh

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Neville Sanjana

@nevillesanjana

7 years

Exciting collaborative work w/ S. Patel & N. Restifo on a human T cell CRISPR screen for cancer immunotherapy:

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Neville Sanjana

@nevillesanjana

2 years

The long-awaited ADULT human brain single-cell paper from @AllenInstitute : "multi-level analysis of over 3 million cells identified 31 superclusters, 461 clusters, and 3313 subclusters." 🤯

@kimsiletti

Kimberly Siletti

2 years

So excited our single-cell transcriptomic survey of the adult human brain is on bioRxiv! @slinnarsson @MossiAlejandro @ka_wai_rine @ErnestArenasSWE A really fun collaboration with Rebecca Hodge, Trygve Bakken, Ed Lein and others at the @AllenInstitute

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Neville Sanjana

@nevillesanjana

3 years

Interested in cutting-edge gene editing? 🧬✂️🔬 The Sanjana Lab @nygenome & @nyuniversity is recruiting a new lab manager. Please RT/forward on! We are looking for a team-oriented scientist w/ skills in mol bio, genomics, stem cells, and/or cancer bio:

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@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

We took a handful of these and found that indeed many of them inhibit viral entry (and are not toxic to cells). Genetic mechanism leads the way to drug discovery! ⚙️💊🙂

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

If Addgene will do for antibodies what it has done for plasmids (both in terms of quality and open sharing), this will be a real game changer. Go @Addgene (and so happy to see that the excellent @AllostEricSite is involved in this too!!)

@AllostEricSite

Eric J. Perkins

@AllostEricSite

3 years

It has been REALLY hard to keep this under wraps. Scientists have been asking me about antibodies since I started doing outreach for @Addgene 12+ years ago. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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Neville Sanjana

@nevillesanjana

4 years

New preprint from our group: “Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens” led by @CathyxGuo with big help from @we_harm , @alexmenman and #DanHaro .

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Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

CRISPR-Cas13 mediates robust transcript knockdown in human cells through direct RNA targeting. Compared to DNA-targeting CRISPR enzymes like Cas9, RNA targeting by Cas13 is transcript- and strand-s...

www.biorxiv.org

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Neville Sanjana

@nevillesanjana

4 years

Fascinating result with implications for all plasmid transfection experiments (not just CRISPR). I would love to know more details on the mechanism here.... there are hints here of a lot more unknown biology! h/t @we_harm

@CommsBio

Communications Biology

4 years

In a study published today in @CommsBio , Kutter et al. present a simple method to improve the efficiency of genome engineering by adding a small vector to a large #CRISPR vector:

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Neville Sanjana

@nevillesanjana

2 years

If you're at #bog22 this year, check out tonight's talk from our postdoc John Morris ( @johnomix ). He's presenting updates to our STING-seq work to perturb GWAS variants w/ CRISPR screens (now +multi-ethnic GWAS & +proteomics!) STING-seq preprint:

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Discovery of target genes and pathways of blood trait loci using pooled CRISPR screens and single...

The majority of variants associated with complex traits and common diseases identified by genome-wide association studies (GWAS) map to noncoding regions of the genome with unknown regulatory effects...

www.biorxiv.org

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Neville Sanjana

@nevillesanjana

2 years

Nothing like 70 degree weather in NYC for a lab ice cream celebration!! 🍦☀️

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Neville Sanjana

@nevillesanjana

5 years

Two impressive new CRISPR-based transposase papers from the groups of @zhangf and @shsternberg in the last week. Evolutionarily distant CRISPR systems (Cas12k and Cascade) with similar functions. Really beautiful new gene editing tools.

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Neville Sanjana

@nevillesanjana

3 years

We have a new preprint on deciphering how noncoding GWAS variants function — both their target genes in cis and networks in trans. @johnonmix who led this work provides a great TWEETORIAL covering all of the highlights below 👇👇👇

@johnomix

John Morris

3 years

Delighted to share work from my postdoc, co-advised by @nevillesanjana and @tuuliel ! It began with wanting to identify target genes for GWAS with CRISPR screens and single-cell seq. Brief look below, with shout-outs to my amazing co-authors! Link:

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Neville Sanjana

@nevillesanjana

4 years

Here’s a link to the manuscript (open access):

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells

To identify potential therapeutic targets for SARS-CoV-2, Daniloski et al. conduct a genome-wide CRISPR screen in human lung epithelial cells. They identify genes and pathways required for SARS-CoV-2...

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@nevillesanjana

Neville Sanjana

@nevillesanjana

3 years

Reassuring to see no SARS-CoV-2 viral integration into the human genome via ONT. 👍 A great use of ONT too (which even with low-pass seq we've found works well for mapping lentiviral integration events).

@biorxiv_genomic

bioRxiv Genomics

@biorxiv_genomic

3 years

Human genome integration of SARS-CoV-2 contradicted by long-read sequencing #biorxiv_genomic

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Neville Sanjana

@nevillesanjana

7 years

New preprint & user-friendly (yet sophisticated) web tool for CRISPR KO screen design (w/ @joshim5 & @zhangf ):

@biorxivpreprint

bioRxiv

@biorxivpreprint

7 years

GUIDES: sgRNA design for loss-of-function screens #bioRxiv

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Neville Sanjana

@nevillesanjana

4 years

CONGRATS! @tuuliel was the first faculty member hired @nygenome and she has been an amazing mentor, scientific collaborator, and co-instructor for our class. I've learned so much from her over the last few years. Here's to many more to my lab neighbor & good friend! 🙂👏

@tuuliel

Tuuli Lappalainen

4 years

It's tenure time! Huge thanks to current and former lab members, collaborators, and many others.

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Neville Sanjana

@nevillesanjana

6 years

Interested in genomics and entrepreneurship? The Genomic Innovation course (co-taught by @tuuliel and myself) will return this fall semester for NYC-area graduate students. Course website:

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Neville Sanjana

@nevillesanjana

3 years

Congrats to PhD student @CathyxGuo for her beautiful cover art to accompany a study led by postdocs @alexmenman & @harm__w on chemically-modified guide RNAs for Cas13. Cathy is a multi-talented genome engineer and artist! 🧬🎨👏

@CellChemBiol

CellChemicalBiology

3 years

The February issue of @CellChemBiol is online. Read it at:

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Neville Sanjana

@nevillesanjana

3 years

Delighted to share our new paper on the importance of tracking cell lineages (w/ @Sophie_Zaaijer and @simoncgroen ). Key issue: In the age of gene editing & patient-derived stem cells, there has been a massive increase in new cell lines for biomedical research. 🔼🔼🔼🧪

@NatureBiotech

Nature Biotechnology

3 years

Tracking cell lineages to improve research reproducibility

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Neville Sanjana

@nevillesanjana

11 months

Beautiful (and personal) history of next-gen sequencing. As an aside: I had no idea that Roger Tsien (of GFP & Ca imaging fame) also first described SBS in 1990! Amazing. (cc @AndrewHires @NathanShaner )

@KrishnanYamuna

Yamuna Krishnan

@KrishnanYamuna

11 months

Raphael Rodriguez and I wrote a historical note on Next Generation Sequencing by Synthesis. We were both postdocs in Shankar’s lab during the time it was being developed at Solexa... 🧵 1/n

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Neville Sanjana

@nevillesanjana

4 years

Very grateful for this support from @CancerResearch and looking forward to this exciting project. Also, congrats to the scientists leading these efforts in the lab (Mat, Xinhe, Zoran, & Maria)!

@nygenome

NY Genome Center

4 years

Congrats to @nevillesanjana of @nygenome / @nyuniversity / @nyuschoolofmed , recipient of a 2020 @CancerResearch Technology Impact Award for #pancreatic cancer immunotherapy research:

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Neville Sanjana

@nevillesanjana

4 years

New Center of Excellence for Genome Science @nygenome — congrats to the entire team! We are lucky to have our lab next door to these amazing folks. 🙌

@satijalab

Rahul Satija

4 years

We are excited to launch the Center for Integrated Cellular Analysis! As part of the NIH @genome_gov CEGS program, we will develop methods to measure and harmonize molecular modalities, spatial context, and lineage history across single cells: (1/3)

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Neville Sanjana

@nevillesanjana

7 months

First case(s) of a lncRNA (CHASERR) haploinsufficiency causing a Mendelian disorder. Super interesting.... so much still to learn about the noncoding genome!

@VGaneshMDPhD

Vijay Ganesh

7 months

Long non-coding RNAs comprise a large part of the genome, yet loss of one copy (haploinsufficiency) of a lncRNA is not known to cause a human disease. Read our preprint to learn how deletions of the lncRNA CHASERR cause a severe neurodevelopmental disorder.

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Neville Sanjana

@nevillesanjana

4 years

Wow — under certain conditions, SARS-CoV-2 RNA can integrate (after RT) into the human genome!

@biorxiv_genomic

bioRxiv Genomics

@biorxiv_genomic

4 years

SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome #biorxiv_genomic

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Neville Sanjana

@nevillesanjana

3 years

Another indication that the Age of Gene and Cell Therapy is now here — amazing to see the long-term durability of these treatments.

@NatureBiotech

Nature Biotechnology

3 years

Ten years after receiving CAR-T therapy, two patients who had a form of blood cancer show no signs of the disease, say @carlhjune and colleagues #NBTInTheirWords via @axios

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Neville Sanjana

@nevillesanjana

4 years

Grateful to @StolteChris for designing the beautiful cover art for our recent paper on pooled CRISPR Cas13 screens in human cells. Here's a link to the study (led by @we_harm and @alexmenman ):

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Massively parallel Cas13 screens reveal principles for guide RNA design

Nature Biotechnology - Design of optimal guide RNAs for Cas13d-mediated RNA knockdown is enabled by computational modeling of large-scale screening data.

@NatureBiotech

Nature Biotechnology

4 years

The June issue is live

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Neville Sanjana

@nevillesanjana

3 years

Preprint alert! 🚨 New work from our group for transcriptome engineering in human cells — including in T cells & to target SARS-CoV-2 — using chemically-modified guides and Cas13 RNPs. Postdocs @alexmenman & @we_harm led the work & here's a great summary from Alex: 👇👇👇

@alexmenman

alexmenman

3 years

Chemically-modified gRNAs can improve CRISPR-based gene targeting and therapeutics. Together with @we_harm , @MatLegut and @nevillesanjana (& in collab with @synthego and @NEBiolabs ), we describe key features of chem-mod crRNAs with RNA-targeting #Cas13 . 1/5

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Neville Sanjana

@nevillesanjana

6 years

This interesting study digs into one puzzling finding from our genome-wide CRISPR screen for resistance to immunotherapy: Why does loss of (many) ribosomal proteins in tumors trigger immunoevasion?

@MolecularCell

Molecular Cell

6 years

Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance

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Neville Sanjana

@nevillesanjana

4 years

We are hiring! We are looking for a RA for in vivo genome editing projects to improve precise gene repair (BS/BA-level candidates with some prior mouse experience). Please RT.

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Neville Sanjana

@nevillesanjana

4 years

This year, @tuuliel and I are again teaching Genomic Innovation, a interdisciplinary class on creating new ventures with cutting-edge genomics. Graduate students from almost any NYC-area school can register:

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Neville Sanjana

@nevillesanjana

3 years

I am grateful to @SFARIorg and excited to be part of this outstanding group of folks working to deliver on the promise of gene therapy. 🚀🧬

@SFARIorg

Simons Foundation Autism Research Initiative

3 years

SFARI intends to fund 17 grants in response to the 2021 Genomics of ASD: Pathways to Genetic Therapies request for applications: #autism #genetics @sandiegobrainia @nevillesanjana @your_arpy @sebatlab @chaolinzhang

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Neville Sanjana

@nevillesanjana

5 years

Congrats @NYGCtech for this month's @naturemethods cover with ECCITE-seq. That is ECCITE-ing! 🙂

@naturemethods

Nature Methods

5 years

The May issue of Nature Methods is live! Read it here:

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Neville Sanjana

@nevillesanjana

2 years

If you're at #ASGCT22 this year, check out today's talk from our postdoc @MatLegut . He's presenting updates to our genome-scale screen for synthetic activators of T cell response (+exciting new data solid tumor work not in the paper!) 🙌 Pub (March 2022):

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A genome-scale screen for synthetic drivers of T cell proliferation

Nature - A genome-scale gain-of-function screen using overexpression of nearly 12,000 open reading frames (ORFs) identifies positive regulators of human T cell function and suggests that ORF-based...

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Neville Sanjana

@nevillesanjana

2 years

Come join our department @nyuniversity ! Two tenure-track positions are open. Top-notch science (basic & translational), wonderful colleagues & a fantastic city!

@justinblau01

Justin Blau

2 years

NYU Biology is hiring: Two tenure-track positions in Molecular, Cellular, Developmental and/or Organismal Biology. Come and be part of our great Department! Apply here: Deadline: November 1st Please RT

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Neville Sanjana

@nevillesanjana

4 years

@tuuliel_lab Highly recommend @zotero — it is free, rock-solid (ahem, Endnote), and has great browser plugins for grabbing refs from the web. The application is very user-friendly and the Word plugin in great.

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Neville Sanjana

@nevillesanjana

3 years

Very proud of this work. Combining a genome scale CRISPR screen with GWAS & eQTL to pinpoint likely causal genes from noncoding variants. Hopefully we can use this approach for many more diseases in the future. Congrats @silvakasela , @tuuliel , @ZDaniloski @virusninja & team!

@tuuliel

Tuuli Lappalainen

3 years

Out paper is now out in @GenomeBiology ! We integrated GWAS, eQTL and CRISPR screen data to pinpoint two genes as potential functional drivers of the biggest COVID-19 human genetic risk locus. Huge congrats to @silvakasela and thanks to all collaborators!

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Neville Sanjana

@nevillesanjana

3 years

Neuroscience and gene editing…. Smart ideas! 👍 Come join our lab @Basketballasart after the bball career 🙂 (And congrats on the amazing win at the Bee! 👏)

@CNNThisMorning

CNN This Morning with Kasie Hunt

@CNNThisMorning

3 years

National Spelling Bee champion Zaila Avant-garde says she is interested in playing basketball at Harvard University, after which she is thinking of four career options: - NBA basketball coach - Working for NASA - Neuroscience - Gene editing

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@nevillesanjana

4 years

We found that top-ranked genes — genes whose loss increases resistance to viral infection — tended to cluster into specific gene families and pathways:

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Neville Sanjana

@nevillesanjana

3 years

Fantastic overview of what's needed to go from academic science to startup. (And at @nyuniversity , we are very lucky to have @ChitaleSadhana as our local tech-transfer expert 🙂)

@NatureBiotech

Nature Biotechnology

3 years

So you want to start a biotech company - @ChitaleSadhana @ArthurKlausner

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Neville Sanjana

@nevillesanjana

4 years

Beautiful genome-wide CRISPR screen of PD-L1 regulation (using the GeCKOv2 CRISPR library) to dissect complex regulation of this immune checkpoint.

@NatureCancer

Nature Cancer

4 years

Have you seen our latest content? 👉 CRISPR screens unveil an eIF5B-mediated integrated stress response-dependent increased translation of PD-L1 in lung cancer - a fascinating work by @shruthy_suresh @ODonnellLab_K Read it here:

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Neville Sanjana

@nevillesanjana

2 years

Wonderful collab w/ @DrBenNeel & Neel lab PhD Wei Wei to identify genes driving SHP2 inhibitor resistance across cancer types. And congrats to co-author @CathyxGuo from our lab! 🙌 There are MANY SHP2 inhibitors 💊 in clinical trials right now 🩺— a very timely study! ⏲️⏲️⏲️

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Neville Sanjana

@nevillesanjana

7 years

We have a search for new faculty (broadly in genetics & genomics) here at @nygenome . Please RT and feel free to reach out and apply! Lots of wonderful colleagues and growing labs here.

@satijalab

Rahul Satija

7 years

We're searching for new a junior core faculty member at the New York Genome Center ( @nygenome )! Pls RT, reach out (and apply!) if interested:

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Neville Sanjana

@nevillesanjana

4 years

Happy to share this fantastic collaborative project with our lab neighbor @EstebanMazzoni . We are very lucky to have Esteban & Gorkem next door! 🙂 In this study, we develop TFome-wide CRISPR libraries to identify key TFs that maintain pluripotency in mouse and human stem cells.

@ggaripler

Gorkem Garipler, PhD

4 years

A Tweetorial on our new results: BTB transcription factors ZBTB11 and ZFP131 repress pro-differentiation genes to maintain #pluripotency .

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Neville Sanjana

@nevillesanjana

6 years

Improved CRISPRi (dCas9-KRAB-MeCP repressor) from @geochurch and colleagues

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Neville Sanjana

@nevillesanjana

5 years

Fascinating — serotonin as a histone modification. "These data identify a direct role for 5-HT, independent from its contributions to neurotransmission and cellular signalling, in the mediation of permissive gene expression."

@LornaFarrelly_

Dr. Lorna Farrelly ✨

@LornaFarrelly_

5 years

Serotonin goes NUCLEAR!! Beyond excited to finally share almost 5 years worth of work. This was an enormous feat, with stellar collaborators @nature

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Neville Sanjana

@nevillesanjana

4 years

Not just for COVID19.... this looks like a good replacement for all qPCR master mixes!

@TjianDarzacq

Tjian + Darzacq Group

4 years

Motivated by the obscenely high cost of #COVID19 testing reagents, we developed #BEARmix 🐻 - an inexpensive, open-source master mix for 1-step RT-qPCR! It can reliably detect 10 RNA copies/rxn and can be made by any lab equipped for prot purif. Protocol👉

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Neville Sanjana

@nevillesanjana

4 years

Interested in bioengineering, CRISPR screens and genome editing in vivo? Then, we are interested in talking to you:

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Neville Sanjana

@nevillesanjana

5 years

Congrats to Rigel, Shashank, @kcw00d , @nrestifo and the rest of the team. Happy to see our 2CT CRISPR screen for immunotherapy resistance finding new targets & therapies!

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@biorxiv_immuno

bioRxiv Immunology

@biorxiv_immuno

5 years

Genome-wide profiling of druggable active tumor defense mechanisms to enhance cancer immunotherapy #biorxiv_immuno

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Neville Sanjana

@nevillesanjana

4 years

Really grateful for all the great #openscience resources from @AllenInstitute . Our lab is a big fan of these amazing (and extraordinarily high-quality) datasets. Thanks to all the AI scientists that make this possible!

@AllenInstitute

Allen Institute

@AllenInstitute

4 years

Equal access to scientific resources is enabled by #openscience . Next Generation Leader @nevillesanjana describes how this is especially beneficial to early-career scientists who bring valuable fresh perspectives to the field. #OpenScienceWeek

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Neville Sanjana

@nevillesanjana

3 years

Genomics chicken & egg problem: Does chromatin accessibility or transcription better define cell state?

@doctorcorces

Ryan Corces

3 years

@hsiung_chris @JeffreyGranja @arjunrajlab The comparison is always going to be biased but I bet if you had eRNAs and splicing/isoform selection, you might find that RNA is more cell type/state specific. But if you take all features, chromatin accessibility is hard to beat esp. when there are so many "housekeeping" genes

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Neville Sanjana

@nevillesanjana

3 years

An amazing gene therapy milestone: Direct in vivo gene editing to reverse a deadly inherited disorder (amyloidosis).

@robsteinnews

Rob Stein

3 years

Another gene-editing milestone—CRISPR injected directly into the bloodstream finds its target.

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Neville Sanjana

@nevillesanjana

3 years

Amazing functional (epi)genomics discovery: For nocturnal animals, chromatin & DNA re-packaging helps photoreceptor cells stay more transparent and block less light. 👀🦉🧬

@jonepstein1

jon epstein

3 years

Nocturnal animals have a trick to see in the dark. They package DNA in rod photoreceptors in a peculiar way to maximize light transmission. It’s an epigenetic trick providing clues to how the genome is normally organized in 3D ⁦ @JonEpsteinLab ⁩

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Neville Sanjana

@nevillesanjana

4 years

If explaining epigenetics and how p53 functions in a direct, cogent way isn't passing a Turing test for biology, I'm not sure what is. It is clear to me that #gpt3 will soon educate more folks on biology than all the faculty in the world. This is the beginning of Wikipedia 2.0.

@vybhavram

Vybhav Ramachandran

4 years

#gpt3 's NLU and reasoning skills are blowing my mind. It's able to explain epigenetic modifications and how it affects gene regulation and it's able to differentiate between acetylation and methylation 🤯 @gdb

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Neville Sanjana

@nevillesanjana

2 years

Beautiful work! Why do tumors go to lymph nodes before metastasizing? To generate immune tolerance. Many important implications here for engineered T cells too...

@RetickerFlynn

Nathan Reticker-Flynn

2 years

Tumors spread to lymph nodes before they metastasize to distant tissues, but for over a century scientists have debated whether this matters. It does. Check out our paper online now @CellCellPress @CellPressNews 1/15

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Neville Sanjana

@nevillesanjana

7 months

Thrilled to work together with @lucksmith on CRISPR (Cas13) functional genomics/transcriptomics in human neurons and grateful to @ChanZuckerberg for supporting our lab!

@nygenome

NY Genome Center

7 months

@NYGenome and Sloan Kettering Institute researchers awarded @ChanZuckerberg grant to collaborate on the study of diverse neuron gene elements in neurodegeneration. Read more on the work of Dr. Sanjana ( @nevillesanjana ) and Dr. Lai ( @lucksmith ) below:

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Neville Sanjana

@nevillesanjana

3 years

So proud of Dr. Congyi Lu from our lab!! Don’t miss her #NYSCF2021 talk on deciphering the TF code for human cortical neuron differentiation. 🧠

@nyscf

New York Stem Cell Foundation Research Institute

3 years

We’re excited to showcase select submitted abstracts as short talks next week at #NYSCF2021 from outstanding junior scientists on #inflammation , #neuroscience , #CardiovascularDisease #CellTherapies , and more! Register here:

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Neville Sanjana

@nevillesanjana

4 years

Using our GeCKOv2 genome-wide CRISPR library (co-developed back in 2013 with @ophirshalem ), we knocked out each of the ~20,000 genes in the human genome.

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Neville Sanjana

@nevillesanjana

4 years

Happy to share that our preprint characterizing a prevalent SARS-CoV-2 Spike mutation is now published: See TWEETORIAL below for a short summary of the work.

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The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types

A pervasive mutation in the Spike protein of the coronavirus that causes COVID-19 results in virions that are up to eightfold more infectious.

elifesciences.org

@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

We recently updated our preprint on the function of the SARS-CoV-2 Spike mutation (D614G). Here’s what we added: 1⃣ Increased infection with the G variant using *real* SARS-CoV-2 virus 2⃣No change in receptor (ACE2) binding using high-res kinetic assays

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Neville Sanjana

@nevillesanjana

4 years

In these turbulent times, it is refreshing to see science at its best and its most hopeful. From a CRISPR screen 6 years ago ( @danielevanbauer , @MatthewCanver , @lucapinello ) to a durable treatment for sickle-cell disease.

@nprscience

NPR Science Desk

4 years

A Year In, 1st Patient To Get Gene-Editing For Sickle Cell Disease Is Thriving

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Neville Sanjana

@nevillesanjana

2 years

Looking for a DNA 🧬 synthesis recommendation: We've been dealing with slow synthesis for gBlocks recently (used to be quite fast). What do others use for gene fragments w/ good speed + price? Typically we need <10 fragments (~1kb size each) per order. Thanks for suggestions!

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Neville Sanjana

@nevillesanjana

6 years

Sanjana Lab 2018 Retreat on Governor’s Island!

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Neville Sanjana

@nevillesanjana

4 years

We recently updated our preprint on the function of the SARS-CoV-2 Spike mutation (D614G). Here’s what we added: 1⃣ Increased infection with the G variant using *real* SARS-CoV-2 virus 2⃣No change in receptor (ACE2) binding using high-res kinetic assays

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The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types

A novel isolate of the SARS-CoV-2 virus carrying a point mutation in the Spike protein (D614G) has recently emerged and rapidly surpassed others in prevalence. This mutation is in linkage disequili...

www.biorxiv.org

@nevillesanjana

Neville Sanjana

@nevillesanjana

4 years

Happy to share our new preprint characterizing a recent mutation in SARS-CoV-2 Spike protein (D614G) and its potential functional impact on the ability of the virus to transduce human cells.

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