Timothy @tim_stasevich Twitter profile | Pikagi

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Timothy

@tim_stasevich

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642

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Fort Collins, CO

Joined September 2014

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@tim_stasevich

Timothy

5 months

A dream of our lab has been to image the full central dogma from a single endogenous gene, all live and with single molecule resolution. After many years we are happy to unveil a beautiful cell line that makes it possible. Check out our preprint ()! (1/n)

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@tim_stasevich

Timothy

1 year

Is there a more clever use of those big paper clips than this? Taguchi lab, Tokyo Tech.

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@tim_stasevich

Timothy

1 year

Ever wonder how modified histones move differently in living cells? Matt Saxton in the lab addresses this question by combining single molecule tracking and FabLEM to fabulous effect:

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@tim_stasevich

Timothy

2 years

What's this? Hint: translation green, mRNA purple.

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@tim_stasevich

Timothy

3 years

One question I get asked a lot is how I get purified proteins into live cells. Answer: bead-loading #justbeadloadit Now we have a video describing the simple procedure. Load your favorite purified protein, DNA, or RNA molecule with little cost or effort!

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Bead Loading Proteins and Nucleic Acids into Adherent Human Cells (Video) | JoVE

Watch this Scientific Journal Video about Bead Loading Proteins and Nucleic Acids into Adherent Human Cells at JoVE.com

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@tim_stasevich

Timothy

4 years

Our paper imaging IRES translation with single molecule resolution in live cells is now out. Nice work from @amandakoch92 , @SysVirology , and Tatsuya Morisaki, in collab with @BMunsky lab.

@NatureSMB

NatureStructMolBiol

4 years

Combination of a bicistronic biosensor w/ single-molecule tracking & computational modelling reveals kinetics of cap- vs IRES-mediated translation & shows that IRES bursts are shorter & rarer than bursts of cap translation, which reverses upon stress

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@tim_stasevich

Timothy

9 months

It's alive! Matt's paper is out today in Science Advances: Congratulations to Matt and all our (Fab)ulous co-authors. More artwork based on data from this paper to come \(^o^)/

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Live-cell imaging uncovers the relationship between histone acetylation, transcription initiation,...

Modified chromatin dynamics are characterized in living cells.

www.science.org

@tim_stasevich

Timothy

1 year

Ever wonder how modified histones move differently in living cells? Matt Saxton in the lab addresses this question by combining single molecule tracking and FabLEM to fabulous effect:

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@tim_stasevich

Timothy

5 months

The result: mesmerizing cells. You can watch transcriptional bursts in the nucleus, follow mRNA out into the cytoplasm, see which ones get translated, and watch mature protein accumulate in situ on demand.

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@tim_stasevich

Timothy

3 years

Excited to learn that I am the first recipient of the Dean and Pingping Tsao Professorship in Biochemistry @CSUNaturalSci ! Also, congratulations to Amanda Koch @amandakoch92 , who received the Kownin award for outstanding graduate student in their final year. Cheers!

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@tim_stasevich

Timothy

9 months

This was fun to make: a graphic showing nucleosomes in areas enriched with H3K27ac diffuse faster than those in areas enriched with RNA Pol II (initiated form). Each image shows all tracks from a single cell. Thank you @CSUSource for promoting our work:

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@tim_stasevich

Timothy

6 years

Got HA-tag? Meet the HA frankenbody and check out our new preprint on imaging HA epitopes in vivo!

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@tim_stasevich

Timothy

3 years

ALERT! ALERT! Anti-FLAG frankenbody on the loose! Believed to have escaped on FLAG day 6/14/2021. Description follows. Spread the word! See also: (1/n)

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@tim_stasevich

Timothy

2 years

Having fun with napari checking out single-mRNA translation spots detected in one of our datasets. Crops of mRNA are arranged with time on the y-axis and mRNA # on the x-axis. I added a signal layer (gray) which shows the quantified translation intensity. @napari_imaging

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@tim_stasevich

Timothy

2 years

Why do I get the feeling the future of mRNA translation imaging is very bright? The string of papers from the Baker lab on de novo designed protein binders in the last few months is seriously mind blowing. Happy holidays for us all.

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@tim_stasevich

Timothy

2 years

Glad to report our Translation n’ Tethering Paper is now out in Nature Communications. Check it out here: It's DYNOMITE! Congrats @ccialek , @MorisakiTatsuya , @NingInScience , Gabe Galindo, and the Tai Montgomery lab!

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@tim_stasevich

Timothy

4 years

(1/16) Let’s get ready to rumble! “IRES the Virus” in blue versus “Cappy” in green: a fight to the death on single mRNA (red) in a live-cell cage match. Details from @amandakoch92 here:

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Timothy

3 years

Excited to give a virtual talk @BU_Biochemistry on Monday (March 1st) at noon EST. I'll discuss the ins and outs of imaging translation dynamics with single molecule resolution. I'll also discuss @amandakoch92 's imaging of IRES translation. Hit me up if you want the zoom invite!

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@tim_stasevich

Timothy

4 years

The Stasevich lab is at the 2020 Biophysical Society meeting @bps2020 in San Diego! Meet up on Sunday: 8:15 am Ballroom 20D -- Tim (IRES vs cap translation) 4:15 am Room 24ABC -- @linda_forero (single gene pol II Ser5ph) & 5 pm @ccialek (miRNA-mediated Ago2 mRNA silencing)

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@tim_stasevich

Timothy

4 years

Read our review on imaging single mRNA translation dynamics in living cells, authored by 3 of our grad students: Amanda Koch ( @amandakoch92 ), Charlotte Cialek ( @ccialek ) and Gabe Galindo (edited by @kerstinbys ). Features new developments and applications.

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@tim_stasevich

Timothy

2 years

So happy to receive a blue flame award for the anti-HA Frankenbody! @NingInScience @Cell_Tokyo_Tech @Addgene

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@tim_stasevich

Timothy

2 years

So this begins my year in Tokyo as a @jsps_sns fellow. I will tweet regularly for those interested in Japan now that the country is opening up again. Follow to learn more about studying and doing research here from abroad! View from my apartment below @tokyotech

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Timothy

5 months

While tags now exist to track single protein and mRNA as well as single transcription and translation sites, the tags are rarely combined or edited into endogenous loci. We wanted to fill that gap.

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@tim_stasevich

Timothy

5 years

Got your HA-tag needs covered here: . Enhanced PDF here: . Besides translation, new data includes 1xHA tag imaging (N- and C-terminal), single molecule tracking, and signal amplification in zebrafish.

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A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

Nature Communications - Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the...

@NingInScience

Ning Zhao

5 years

Our HA frankenbody paper is out! Frankenbody is powerful for studying single RNA translation, single molecule tracking and protein localization. Did I mention all of these are in vivo? And I'm thrilled to announce Flag frankenbody is coming soon! @tim_stasevich @Cell_Tokyo_Tech

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@tim_stasevich

Timothy

5 years

Fingers crossed!

@jakedel1

DeLuca Lab

5 years

Awesome tenure talk by ⁦ @tim_stasevich ⁩!! Go Tim and team!!

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@tim_stasevich

Timothy

3 years

Congratulations Dr. Amanda Koch!!

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@tim_stasevich

Timothy

7 years

Flashbody: an awesome fluorogenic scFv that lights up a 7aa C-terminal peptide:

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Flashbody: A Next Generation Fluobody with Fluorescence Intensity Enhanced by Antigen Binding

Fluorescent probes are valuable tools for visualizing the spatiotemporal dynamics of molecules in living cells. Here we developed a genetically encoded antibody probe with antigen-dependent fluores...

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@tim_stasevich

Timothy

5 years

Single nucleosome tracking is getting more powerful! I imagine this technique will become a standard for cell biologists studying chromatin.

@kazu_maeshima

Kazuhiro Maeshima

5 years

Collaboration work with Ashwin-san and Sasai-san at Nagoya is online . We statistically analyzed our single nucleosome imaging data , and found fast and slow nucleosomes. We can extract more and more information from our data!

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@tim_stasevich

Timothy

11 months

Our department is hiring! Come join us in Fort Collins, Colorado. Great environment for research and play. We encourage those studying biological processes using cell biology, imaging, and/or biophysical and structural approaches to apply.

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Faculty Position: Tenure-Track Assistant Professor in Cellular Biochemistry and/or Biophysics -...

Assistant Professor Position in Cellular Biochemistry and/or Biophysics in the Biochemistry and Molecular Biology Dept. at Colorado State University.

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@tim_stasevich

Timothy

4 years

Great work all! It still amazes me we can do fluorescence correlation analysis of endogenous Pol II phosphorylation at a single-copy gene. Thanks to the bursty nature of genes...

@BMunsky

Brian Munsky (he/him/his)

4 years

Super proud of @linda_forero and @tim_stasevich for outstanding work to quantify spatiotemporal RNAP2 phospho-dynamics at single-gene resolution and of Will Raymond for finding a simple mechanistic model to capture all data. The fits are textbook!

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@tim_stasevich

Timothy

5 years

Congratulations to Dr. Kenneth Lyon! My first graduate student and now first lab alumni! Great job. Super proud and looking forward to seeing your cool frameshift paper in print soon!

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@tim_stasevich

Timothy

4 years

Undergrad/post-bac interested in RNA and grad school in Colorado? Join the Colorado RNA Club on Oct 20th to learn more about local graduate programs from CU Boulder & Anschutz, CSU, MSU Denver, and DU. Panel discussions, research talks, and breakout rooms. RSVP below. Please RT!

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Timothy

3 years

Looking forward to this. Only wish I could come in person!

@umichrna

UMich RNA Center 〽️🧬

3 years

Mon.Oct 18, 4 ET, our #RNA Innovation Seminar Series with Tim Stasevich, Ph.D., @tim_stasevich @ColoradoStateU Keywords: translational regulation, gene expression, fluorescence microscopy, intrabodies, single-molecule imaging @ColoradoRNAClub REGISTER:

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@tim_stasevich

Timothy

7 months

Beautiful work from Hiroshi Ochia's lab demonstrating the raw power of many rounds of DNA/RNA/IF-FISH, all at an edited endogenous locus where the transcriptional status can be independently assessed. So cool!

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Transcription-coupled changes in higher-order genomic structure and transcription hub viscosity...

The orchestration of our genes heavily relies on a coordinated communication between enhancers and promoters, yet how this dynamic interplay remains elusive while transcription is active. Here, we...

www.biorxiv.org

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Timothy

3 years

This is definitely the best part about being an advisor 😃. Truly well deserved Ning! Can't wait to see where you will go with this project. Super exciting!

@NingInScience

Ning Zhao

3 years

I'm thrilled to announce that I'm a NIH K99/R00 awardee! Many thanks to everyone who helped me in this journey! Special thanks to NIH, my mentor Tim Stasevich, CSU TagTeam (Chris Snow and Brian Geiss), and my department! @NIGMS @tim_stasevich @Christo56968626 @GeissLaboratory

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Timothy

5 years

Nature is amazing.

@MolecularCell

Molecular Cell

5 years

A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly

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@tim_stasevich

Timothy

6 years

Proud of this work with Brian Munsky ( @BMunsky ) which provides a first peak at non-canonical translation in living cells at the single RNA level: A companion preprint from the Tanenbaum lab looks at start codon selection:

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Live-cell single RNA imaging reveals bursts of translational frameshifting

Ribosomal frameshifting during the translation of RNA is implicated in both human disease and viral infection. While previous work has uncovered many mechanistic details about single RNA frameshift...

www.biorxiv.org

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Timothy

5 months

At the level of transcription, we can see up to four active alleles (consistent w/ a diploid locus), with long, many-hour ‘off’ times interspersed by highly bursty ‘on’ times.

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@tim_stasevich

Timothy

2 years

Finally arrived back in Japan after 3 years! Super excited to be @tokyotech_en for the next 10 months on sabbatical. And soooo glad 100 yen price down vending machines made it through the pandemic.

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@tim_stasevich

Timothy

2 years

We were lucky to host @Weihan_Li_ this week from the Singer lab. Really engaging talk on improved mRNA imaging in yeast w/ some VERY cool applications!

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@tim_stasevich

Timothy

2 years

Awesome to be out with the lab at the CSHL Single Biomolecules meeting. Been blown away by all the beautiful work. With Matt Saxton, Gabe Galindo, and @NingInScience . @cshlmeetings #cshlsingmol

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Timothy

1 year

This corner of campus at Tokyo Tech feels straight out of a Lost episode. Should I enter the tunnel or take the steps?

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@tim_stasevich

Timothy

4 years

It's Philly rolls tonight!

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@tim_stasevich

Timothy

3 years

So proud of these two!

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@tim_stasevich

Timothy

4 years

That feeling you have when a paper you submitted to biorxiv two years ago finally gets accepted for publication after ten rounds of revision.

@AP

The Associated Press

4 years

JOE BIDEN DEFEATS PRESIDENT DONALD TRUMP The Associated Press declares Joe Biden the winner of a grueling campaign for the American presidency. He will lead a polarized nation through a historic collision of health, economic and social crises. #APracecall

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@tim_stasevich

Timothy

3 years

Get your TnT (Tethering n' Translation) biosensor today to add some spice to your imaging life!

@ccialek

Charlotte

3 years

9/9 Check out our pre-print to learn more: 🔥🧨 TnT – It’s dynamite! 🧨🔥

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@tim_stasevich

Timothy

5 years

This was a great collaboration between our lab and the Parker lab at CU Boulder. Thanks especially to Stephanie @smslmoon and Tatsuya for your hard work. It's been a pleasure and looking forward to more awesome collaborative science!

@smslmoon

Stephanie Moon

5 years

Excited to share our paper just published today @NatureCellBio ! We imaged single mRNAs in living human cells to monitor translation and interactions with stress granules and P-bodies #RNA #stressgranules @tim_stasevich @anthonykkhong @BioKenLyon

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Timothy

1 year

Wonderful baseball yesterday, Japan. And wonderful cherry blossoms too!

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@tim_stasevich

Timothy

5 years

Amazing sunset tonight swimming in the ocean with Fuji-san as a backdrop. Awesome!

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@tim_stasevich

Timothy

5 months

O’Neil Wiggan took on this challenge by doing 2 rounds of CRISPR to track MYH9 gene expression. MYH9 encodes myosin-2a, a critical cytoskeletal motor that pushes/pulls actin so cells can move, reorganize, divide, and differentiate (cartoon adapted from Brito et al, Cells, 2020).

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@tim_stasevich

Timothy

3 years

nanobody, scFv, Fab, mAB

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@tim_stasevich

Timothy

5 months

It's been a wonderful #BPS2024 so far. Now showing: optogenetic control of single-molecule translation from Gabe Galindo and Gretchen Fixen. Poster #LB91

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@tim_stasevich

Timothy

4 years

And don't forget graphical abstracts, Twitter storms, commissioned cover art, full-length 3D movies with zoom views and special effects, the press release, ...

@VirusesImmunity

Prof. Akiko Iwasaki

@VirusesImmunity

4 years

What it takes to publish a paper has dramatically changed over the past 2-3 decades. The 2020 expectation of exponential # figure panels required to publish a paper is getting a bit ridiculous, especially given the fact that NIH R01 modular cap of $250K hasn't changed since 1999.

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Timothy

2 years

The mood in Fort Collins...

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Timothy

3 years

Awesome, we got plasmid of the day today!

@Addgene

Addgene

3 years

Multi-frame tag Depositor: Tim Stasevich @tim_stasevich Purpose: In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

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Timothy

4 years

Oh yeah, more awesome news today! Our department is hiring at the Assistant or Associate Professor level. RT and spread the word. Come join us in beautiful Colorado.

@lab_markus

Markus Lab

4 years

In OTHER news today, I'm excited to announce that my department is hiring! We are seeking "...individuals with research interests and backgrounds in any area of Biochemistry and Molecular Biology." Interested in joining amazing colleagues? Apply!

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@tim_stasevich

Timothy

5 months

Thanks to @ColoradoStateU , @CSUNaturalSci , @tokyotech_en , all Stasevich lab members, and funding from the NIH and the NSF for making this work possible. If you found this interesting, please retweet!

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@tim_stasevich

Timothy

3 years

Had fun being immersed in van Gogh's art during my trip to Michigan. Wouldn't this be cool with live-cell microscopy? Anyone wanna help me make that happen!?

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Timothy

3 years

Nice lunch with lab at the wonderful outdoor dining area at Rainbow, in Fort Collins.

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Timothy

2 years

Here's the main street by the station near Tokyo Tech's main campus. Nice old-school Japan vibe that feels straight out of a movie. Love that pedestrians and not cars rule here.

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Timothy

2 years

For my @NSF award, a goal is to introduce open-source video microscopy to kids. For this, we created a kid-friendly lensless microscope (Shadow Scope). Now I'm making mascots (the Silhouettes). Can you help me name them? I'll attach their images below. @CSUShadowScope

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@tim_stasevich

Timothy

5 years

Our new paper using a multi-frame tag to visualize single RNA frameshifting is out. Fantastic summary from the always great @AnneJuManning here:

Dynamics of key viral attack strategy visible for first time

Researchers have developed imaging technology and computational analyses to visualize viral frameshifting at the level of single molecules in living cells.

natsci.source.colostate.edu

@BioKenLyon

Kenneth Lyon

5 years

Woohoo! My work in the Stasevich lab. Myself, @tim_stasevich , @BMunsky , Luis Aguilera, and Tatsuya Morisaki were able to visualize and quantify single RNA frameshifting dynamics for the first time!

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Timothy

4 years

Check out the great talk from @amandakoch92 about IRES-mediated translation! #RNA2020

@amandakoch92

Amanda Koch

4 years

To all the #RNA2020 attendees, please check out my talk about how we looked at single IRES-mediated translation events in living cells in Plenary Session 4: Ribosomes&Translation. Email or tweet at me with ?s! #translation #Science #VirtualConference

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Timothy

5 months

At the level of translation, we see tens to hundreds of dancing polysomes, most diffusing, some directed, others localized. Sometimes we see polysomes in multi-RNA clusters that are robustly translated.

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Timothy

4 years

Get your frankenbody this Halloween to do some scary-cool science!

@Addgene

Addgene

4 years

Meet the HA frankenbody, a genetically encoded antibody based probe that detects HA epitopes. Read about how it was made & its applications (hint: like Frankenstein the frankenbody was constructed by fusing diff parts together) @Ning31502395 @tim_stasevich

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Timothy

2 years

I often get asked if our anti-HA frankenbody scFvs can be used in yeast. Now there's an answer! Check out this cool paper for great applications and good tagging strategies to light up yeast HA tags in a variety of settings. Congrats and many thanks to the authors!

@IoannisChemist

Ioannis Tsirkas

@IoannisChemist

2 years

This was the most fun project I ever had! By utilizing a previously published optimized anti-HA single-chain Ab (Frankenbody), we developed a methodology to fluorescently HA-tagged proteins in live S. cerevisiae cells. 1/ @TomerZur6 @lab_amir #Microscopy #yeast @CellRepMethods

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Timothy

4 years

Looking forward to this talk!

@cplcillinois

CPLC

4 years

Join us THIS Friday at 2pm, for a talk by Tim Stasevich, "Real-time quantification of gene expression with single-molecule precision in living cells". Email ipols @cplc .illinois.edu for the Zoom link.

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Timothy

6 years

Dock, lock, n' roll: translationally silent mRNAs in stress granules do cool things! See for yourself @ with Parker Lab and @smslmoon .

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Timothy

4 years

(1/16) Let’s get ready to rumble! “IRES the Virus” in blue versus “Cappy” in green: a fight to the death on single mRNA (red) in a live-cell cage match. Details from @amandakoch92 here:

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Timothy

4 years

I like ribosomal traffic jams and skiing, so this article is for me!

@MolecularCell

Molecular Cell

4 years

Online Now: Extraction of mRNA from Stalled Ribosomes by the Ski Complex

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@tim_stasevich

Timothy

5 years

More fun translation imaging news: for those wanting to explore codon usage and simulate single RNA imaging experiments, check out this paper and download the open-source rSNAPsim tool! Super easy to check any gene from database and play around with codon usage.

@BMunsky

Brian Munsky (he/him/his)

5 years

Check out our newest collaboration with the @tim_stasevich lab using stochastic models to design and interpret single-RNA translation experiments. Great work Luis, Will, and the rest of rSNAPsim team! bioRxiv viewer

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Timothy

5 months

We show MYH9 transcription is SRF dependent and relies on the nuclear import of MLK-1 when G-actin levels are low relative to F-actin.

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Timothy

3 years

So happy to be a part of this project to make and share recombinant antibodies. Thanks @jakedel1 !

@jakedel1

DeLuca Lab

3 years

Our antibody paper is up on bioRxiv! … We describe tools & methods for making low-cost, high-yield recombinant mAbs and derivatives e.g. fragments and genetically-encoded nanobodies…1/7

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Timothy

2 years

Going through my old SCT data (single child tracking).

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@tim_stasevich

Timothy

4 years

#Mandolorian take note: ~300 nm isotropic imaging of yeast with our axially swept Pi-SPIM microscope is a cost-effective* way to simulate flying around desert huts on Tattoine. Here's an attempted threading of the yeast needle and the inevitable crash... *microscope not included

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Timothy

5 months

All in all, our data soundly demonstrate MYH9 transcription and translation are on separate dials. But still so much to be explored with these cells. If you think they could be useful for your research, please let us know! We’d love to share them with you.

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@tim_stasevich

Timothy

1 year

Wait .. What!?

@LugerLab

Karolin Luger

1 year

News alert: Some bacteria have histones y'all. Shawn Laursen from @LugerLab shows how they bind DNA in VERY unexpected way, defying common 'histone logic'. Check out our preprint with @tobias_warnecke @antoine_hocher and Socket lab . Very exciting!

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Timothy

5 months

In round two he added a heterozygous N-terminal 6xFLAG tag so we could visualize translation in a third color using @NingInScience ’s anti-FLAG frankenbodies (or Fab/AB)!

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Timothy

4 years

My son worked really hard making a YouTube video about solving the Rubik's cube. It'll make his New Year if you like, share, and subscribe! Kids content for kids!

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@tim_stasevich

Timothy

5 years

Get your frankenbody fix on Addgene here!

@Addgene

Addgene

5 years

Meet the HA frankenbody, a genetically encoded antibody based probe that detects HA epitopes. Read about how it was made & its applications (hint: like Frankenstein the frankenbody was constructed by fusing diff parts together) @Ning31502395 @tim_stasevich

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Timothy

5 months

Transcription + translation increase just before mitosis, but then translation shuts down until division is over.

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@tim_stasevich

Timothy

5 months

We also uncovered a hidden translational buffering system. Upon the loss of an actin depolymerizing protein cofilin, MYH9 transcription goes up, but myosin-2a translation efficiency drops by a factor of two to partially counter the response.

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Timothy

5 months

Cool sidenote: to find local translation sites in the midst of all the mature myosin-2a, we look for mRNA spots with FLAG signals but lacking GFP (since GFP is not yet fluorescent during translation).

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Timothy

5 years

More cool probes from Tokyo Tech to image histone modifications. Here FRET was combined with an scFv mintbody to create a probe that brightens upon binding. @YukoSatoT2 @Cell_Tokyo_Tech

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Intrabody-based FRET probe to visualize endogenous histone acetylation

Scientific Reports - Intrabody-based FRET probe to visualize endogenous histone acetylation

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Timothy

2 years

Congratulations to you Ning! It's been a fantastic journey. Can't wait to see all the great things you do in your lab. ^_^

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Timothy

2 years

This is a very nice paper showing how nanobodies can be generally stabilized via a few key mutations. Same approach could be applied to scFvs: A general approach for stabilizing nanobodies for intracellular expression

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A general approach for stabilizing nanobodies for intracellular expression

A large proportion of nanobodies are unstable when expressed intracellularly, but can be reliably stabilized for intracellular expression by applying a generalizable mutagenesis strategy based on...

elifesciences.org

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@tim_stasevich

Timothy

1 year

Next-level Japanese experience: after karate class yesterday (where I regularly make a fool of myself), I went to the local onsen to heal. Inside there were four sumo wrestlers in training! Felt like another era.

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@tim_stasevich

Timothy

2 years

For those interested in research in Japan, first a little background on me: My love affair with Japan goes way back to a summer REU program in the late '90s, where I met my wife (she's Japanese) at @stonybrooku .

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Timothy

4 years

For fluorescence microscopy tweets (like @rhutto3 ) who like to keep their slides with black backgrounds, here's a simple way (w/ mathematica) to invert grayscale images while leaving red, green, and blue (almost) alone. Anyone wanna make Matlab, Python, and Fiji versions?

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@tim_stasevich

Timothy

11 months

Excited to be in Seoul for the International Conference on Biological Physics. Near Olympic Park (left). Cool view from my hotel room (right). Remembering the 88 Olympics and, in the US at least, Greg Louganis diving, hitting his head, and winning was inspiring. #ICBP2023

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Timothy

3 years

Introducing Dr. Charlotte Cialek ( @ccialek )! Latest PhD, joint from from my lab and Tai Montgomery's lab at @CSUNaturalSci . Your committee (Erin Nishimura @ErinAsayo , Fred Hoerndli, and Eric Ross) congratulates you!

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Timothy

4 years

I like this and we do need more model organisms, but not sure it's so easy (I tried daphnia once). Reminds me of a funny manual to float: 1) meditate, check; 2) feel yourself getting lighter, check; 3) float! Seriously, though, this is a great motivation:

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Timothy

5 years

Meandering Kyoto streets last night, managed to find this Hoko float. The Gion Matsuri festival has a 1000+ year history! The Hoko was beautifully lit up at night. Relics on the side are ancient, used to decorate the float every year.

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Timothy

5 months

In round one he homozygously knocked in a C-terminal GFP+MS2 tag so we could image all mature myosin-2a protein in one color and mRNA in another.

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Timothy

5 months

Interestingly, although we might guess the translation signals on each mRNA would get 50% dimmer, instead their signals remained pretty much unchanged, with just half as many mRNA being translated. Couldn’t see this without the single-mRNA resolution!

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Timothy

4 years

Happy to be a part of this work from Markus Affolter's and Giorgos Pyrowolakis' labs: a fair comparison of SunTag, MoonTag, HA tag, and Alfa-tag for live-cell imaging. If you're using an HA-tagged fly line, check out degradHA using the HA frankenbody (like degradFP).

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Timothy

5 years

Was awesome hanging with Tim Lionnet in his fabulous lab yesterday and also hanging with Brian English! Was a great reunion. @successprocess @sciencethecat

@sciencethecat

Brian English🔬

5 years

i got to visit Timothée Lionnet and his awesome lab. reunion of old #janelia friends @successprocess @tim_stasevich 🔬

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Timothy

2 years

Watch out Tokyo, a couple new monsters are in town.

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@tim_stasevich

Timothy

3 years

A notable american elm on campus. They say these trees are tetraploid, having double the number of normal chromosomes. Sad to think how many succumbed to dutch elm disease. Glad this one is here.

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Timothy

4 years

My wife made a facemask for all of us. Mia is ready!

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@tim_stasevich

Timothy

11 months

Awesome talks yesterday at #ICBP2023 . In the evening I had a chance to walk around and think/digest it all. Here's a nice view of the Lotte tower in Seoul. 5th tallest building in the world.

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