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Guillaume Jacquemet Profile
Guillaume Jacquemet

@guijacquemet

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Cell biologist interested in Actin cytoskeleton and cancer cell invasion. My opinions are my own.

Turku, Finland
Joined October 2009
Don't wanna be here? Send us removal request.
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@guijacquemet
Guillaume Jacquemet
2 months
Delighted to share the latest paper from the lab. "TLNRD1 is a CCM complex component and regulates endothelial barrier integrity" It's been a very long journey to get this work from initial discovery to publication and I am very proud of the final product
@sujanghm
Sujan Ghimire ๐Ÿ‡ณ๐Ÿ‡ต๐Ÿ‡ซ๐Ÿ‡ฎ
2 months
Got the perfect B'day gift! ๐ŸŽ‰ Proud to share as a co-first author, our latest work on TLNRD1 and its crucial role in vascular integrity, now published in @JCellBiol ! Big thanks to Neil J. Ball, @guijacquemet @bengoult and all contributors.
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@guijacquemet
Guillaume Jacquemet
4 years
Movie I recorded a few years ago, but still one of my favorites. Focal adhesion dynamics in endothelial cells during mitosis. Perfect for #FocalAdhesionFriday
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@guijacquemet
Guillaume Jacquemet
5 years
Delighted to announce that I will start my lab @aboakademi #turku #finland in September. We will be studying how cells interact with their environment using microscopy and mass-spectrometric approaches ! Looking for a PhD or a master project ? be in touch !
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@guijacquemet
Guillaume Jacquemet
3 years
Wow, this is huge: OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization Preprint: Web portal
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@guijacquemet
Guillaume Jacquemet
3 years
#DeepLearning is a powerful method to analyze bioimages. But, How to check that it works well (enough) on your data? How to ensure reproducibility? What should be reported in publications? @LaineBioImaging @IgnacioArganda @HenriquesLab and I wrote a thing:
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@guijacquemet
Guillaume Jacquemet
3 years
It's been quite a week, delighted to welcome our newest member! Olivia, born yesterday. Everyone is doing well ๐Ÿ˜Š
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@guijacquemet
Guillaume Jacquemet
7 years
It's finally out: Our @NatureProtocols CDMs for studying cell proliferation and directional migration @ivaskalab
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@guijacquemet
Guillaume Jacquemet
2 years
Delighted to share the latest contribution from my lab "TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines" A wonderful collaboration with @jytinevez Work done @AboAkademi by @JwPylvanainen and myself on our side
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@guijacquemet
Guillaume Jacquemet
7 years
It's #focaladhesionfriday look at this little guy divide !
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@guijacquemet
Guillaume Jacquemet
4 years
Spinning cancer cells. Cells growing in 3D, labeled for Actin.
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@guijacquemet
Guillaume Jacquemet
11 months
Do you work with cell migration data? Or do you track objects from microscopy video? You may be interested in my latest project: CellTracksColab, A platform for compiling, analyzing, and exploring tracking data
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@guijacquemet
Guillaume Jacquemet
5 years
Tail of a zebrafish embryo stained for collagen
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@guijacquemet
Guillaume Jacquemet
3 years
Playing with 3D segmentation today. Not too shabby.
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@guijacquemet
Guillaume Jacquemet
2 years
It's official, I am now an Associate Professor as part of #SolutionForHealth @AboAkademi . I am also joining the wonderful @InFLAMES_Health crowd. Lots of exciting days ahead! Of course, still hanging out with folks @BioscienceTurku & @turkubioimaging
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@guijacquemet
Guillaume Jacquemet
4 years
We wrote a small preprint describing how we combine #StarDist and #TrackMate for automated cell tracking (full step-by-step protocol). Contributions from @mycroscopy @roybiolab @Follain_Ga @LaineBioImaging @ChamierLucas @pekkahanninen @jeriksso1 @jytinevez
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@guijacquemet
Guillaume Jacquemet
11 months
Delighted to share our latest review: "Live-cell imaging in the deep learning era" Written with @JwPylvanainen @gomez_mariscal and @HenriquesLab
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@guijacquemet
Guillaume Jacquemet
4 years
Stunning images of breast cancer cells taken by @Annaserafia (Actin staining, Airyscan confocal microscope)
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@guijacquemet
Guillaume Jacquemet
4 years
Interested in combining #StarDist and #TrackMate for automated cell tracking. Want to process whole folders? I have now added an R script to automatically compile all your data!
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@guijacquemet
Guillaume Jacquemet
5 years
I have spent the last 3h reading through CV and motivations letters (For a PhD position still open so apply). I am by no mean an expert but here is a list of things you not do when you apply somewhere. Hope this is helpful to someone. (a thread)
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@guijacquemet
Guillaume Jacquemet
6 years
A very satisfying image. Temporal colour coded image of filopodia dynamics
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@guijacquemet
Guillaume Jacquemet
3 years
Many thanks, @SuomenAkatemia for funding our work! Looking forward to working with my team on "Filopodia-mediated communication in cancer progression" during the next five years at @AboAkademi @BioscienceTurku
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@guijacquemet
Guillaume Jacquemet
7 years
Ladies & Gentlemen, @joachimgoedhart mTurquoise2-Lifeact imaged on our #airyscan @zeiss_micro
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@guijacquemet
Guillaume Jacquemet
2 years
Latest from us: Fast4DReg: Fast registration of 4D microscopy datasets We've struggled for a while with the registration of 4D datasets, so we made a thing. Awesome work by @JwPylvanainen @LaineBioImaging @sujanghm @Follain_Ga @HenriquesLab
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@guijacquemet
Guillaume Jacquemet
5 years
My latest contribution: Cell matrix adhesion in cell migration Written with James Conway @IvaskaLab If you cannot access it feel free to contact me !
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@guijacquemet
Guillaume Jacquemet
3 years
I discovered this amazing 3D visualisation tool last night: The @FijiSc integration is especially brilliant Works beautifully on my GPU less laptop (where everything else crashes). Kudos to the developers
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@guijacquemet
Guillaume Jacquemet
2 years
And we got the cover! Many thanks @J_Cell_Sci
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@guijacquemet
Guillaume Jacquemet
2 years
Our #Fast4DReg paper is now available at @J_Cell_Sci . Fantastic work driven by @JwPylvanainen with contribution from @sujanghm @Follain_Ga Amazing collaboration with @LaineBioImaging @Bruno_MSaraiva and @HenriquesLab
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@guijacquemet
Guillaume Jacquemet
3 years
Latest from the lab!! Myosin-X and talin modulate integrin activity at filopodia tips @mitroe @GronlohMax @anapopovic0503 @EJokitalo Helena Vihinen @bengoult @johannaivaska
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@guijacquemet
Guillaume Jacquemet
7 years
Recyclage of an old poster as a table cloth ! Perfect for a cheese fondue
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@guijacquemet
Guillaume Jacquemet
4 years
#FocalAdhesionFriday Yes, cells can be very flat! (Focal adhesion in magenta; Actin in green; Filopodia tips in Red). 3D SIM.
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@guijacquemet
Guillaume Jacquemet
8 months
Interested in (cell) tracking analyses? Major update to the #CelltracksColab preprint. New figures, new analysis modules, and new datasets. Everything is openly available and a few clicks away.
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@guijacquemet
Guillaume Jacquemet
4 years
Happy new year everyone!
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@guijacquemet
Guillaume Jacquemet
4 years
Automated nuclei tracking using #Stardist and #Trackmate . 1st) Stardist using #ZeroCostML4Mic 2nd) Stardist result loaded in @FijiSc and automatically track using a Trackmate script. Next, R script to analyse the data! Thanks, @eli_fazeli for the help!
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@guijacquemet
Guillaume Jacquemet
5 years
Many thanks to @SigridJuselius for providing me with the funds to kick-start my independent career ! I will be looking for a PhD student soon
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@guijacquemet
Guillaume Jacquemet
7 years
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@guijacquemet
Guillaume Jacquemet
2 years
Interested in image analysis? Interested in a Master/Ph.D./Postdoc in Finland with us? Please be in touch!!
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@guijacquemet
Guillaume Jacquemet
4 years
We are finally moving toward CRISPR - mediated endogenous tagging (vimentin here). Very well done @Annaserafia ! Using the tech described in @JCellBiol here:
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@guijacquemet
Guillaume Jacquemet
4 years
I uploaded the #Trackmate script we use to automatically track cell migration movies (detection performed using #Stardist ) in case it can be useful for someone else.
@guijacquemet
Guillaume Jacquemet
4 years
Automated nuclei tracking using #Stardist and #Trackmate . 1st) Stardist using #ZeroCostML4Mic 2nd) Stardist result loaded in @FijiSc and automatically track using a Trackmate script. Next, R script to analyse the data! Thanks, @eli_fazeli for the help!
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@guijacquemet
Guillaume Jacquemet
6 years
As scientists we spend a fair amount of time reproducing published work. Some of these results get published, most doesn't. Why not create a platform where we could tag experiments that have been successfully reproduced? Link tag to published papers and ORCID
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@guijacquemet
Guillaume Jacquemet
4 years
I have now updated our @github page to include a short step by step tutorial on how to automatically track cell nuclei movies by combining our #Stardist #ZeroCostDL4Mic notebook with #Trackmate If anything is unclear do not hesitate to reach out!
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@guijacquemet
Guillaume Jacquemet
7 years
It's out! @JCellBiol FiloQuant reveals increased filopodia density during breast cancer progression
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@guijacquemet
Guillaume Jacquemet
2 years
and one of my images made the cover!! Lifeact-mRFP Ductal carcinoma in situ-like xenografts (d 25) were dissected and incubated with the fibrillar collagen probe CNA35-GFP (blue and gold) before imaging using a spinning-disk confocal microscope.
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@guijacquemet
Guillaume Jacquemet
2 years
It's out ๐Ÿฅณ!! Latest paper from the lab "MYO10-filopodia support basement membranes at pre-invasive tumor boundaries": a fantastic collaboration with @IvaskaLab @johannaivaska and @EPeuhu with contribution from many others
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@guijacquemet
Guillaume Jacquemet
2 years
Love microscopy ๐Ÿ”ฌ? Could not join #ELMI2022 ? Fear not, I will be posting most of the #ELMI2022 talks on the @turkubioimaging youtube channel over the summer #OpenAccess
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@guijacquemet
Guillaume Jacquemet
5 years
A new beginning !
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@guijacquemet
Guillaume Jacquemet
3 years
#min รคtutkin At @BioscienceTurku @AboAkademi , my lab study how cancer cells move to block cancer metastasis. We also develop technologies and train the next generation of scientists Without funding from @SuomenAkatemia @SigridJuselius @Syopajarjestot we would not be able to work
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@guijacquemet
Guillaume Jacquemet
3 years
While I like my current job, I really miss being a postdoc.
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@guijacquemet
Guillaume Jacquemet
4 years
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@guijacquemet
Guillaume Jacquemet
4 years
I have been playing with deep learning and prediction of fluorescence signal from brightfield images. Which of these images was generated by a computer A or B ? #DeepLearning #ZeroCostDL4Mic Thanks to @miCHRIScopy for the nice raw data! (colour indicate depth).
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@guijacquemet
Guillaume Jacquemet
6 years
My latest paper done @ivaskalab is out ! I really enjoyed working on that one ! many thanks @akistub @mitroe @hellyeh_h @johannaivaska Rafael and Elena @LabLappalainen
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@guijacquemet
Guillaume Jacquemet
3 years
Fresh from the SIM!
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@guijacquemet
Guillaume Jacquemet
5 years
Hot from the press : Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells. This has been a very long journey. Very proud of @akistub and Camilo for pushing it through the finish line! Glad to have been able to help !
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@guijacquemet
Guillaume Jacquemet
2 years
Our #Fast4DReg paper is now available at @J_Cell_Sci . Fantastic work driven by @JwPylvanainen with contribution from @sujanghm @Follain_Ga Amazing collaboration with @LaineBioImaging @Bruno_MSaraiva and @HenriquesLab
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@guijacquemet
Guillaume Jacquemet
6 years
Happy Wednesday !
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@guijacquemet
Guillaume Jacquemet
4 years
The first version of our #Detectron2 #ZeroCostDL4Mic notebook is almost ready to share! #GeekySunday Read more about #Detectron2 here
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@guijacquemet
Guillaume Jacquemet
6 years
Unpopular opinion: a postdoc isn't a training to become a PI. It's a full time research job.
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@guijacquemet
Guillaume Jacquemet
7 years
Video 10 is stunning ! Such beautiful filopodia and retraction fibres Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms
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@guijacquemet
Guillaume Jacquemet
3 years
It's up! "Myosin-X-dependent assembly of the extracellular matrix limits breast cancer invasion" A wonderful collaboration with @EPeuhu @johannaivaska @IvaskaLab @EloLab_fi @vanRheenen_Lab and several others Several unexpected findings, a ๐Ÿงตto follow soon
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@guijacquemet
Guillaume Jacquemet
3 years
Using #StarDist together with #TrackMate v7 to track filopodia tips. Needs a bit more tunning but the first results are awesome.
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@guijacquemet
Guillaume Jacquemet
3 years
Delighted to announce that our #ZeroCostDL4Mic paper is now available in its post-peer review version. It's been an incredible and transformative journey for me and my lab. Only possible thanks to daily interactions with @HenriquesLab @LaineBioImaging and @ChamierLucas
@LaineBioImaging
Romain F. Laine
3 years
We are pleased to announce that our #ZeroCostDL4Mic work is now published! And our @github #DeepLearning and #Microscopy for all! For the occasion, Iโ€™ve put a small Tweetorial together to explain what this is all about! Thread 0/7
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Guillaume Jacquemet
2 years
Thanks for asking @eLife but actually I haven't published my best work in your journal. Your editors editorially rejected my last four tentatives, so I published elsewhere ๐Ÿ˜‚
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@guijacquemet
Guillaume Jacquemet
4 years
A new #ZeroCostDL4Mic notebook is now available: DRMIME which can be used to perform 2D image registration (affine or perspective). In the lab, we are now using it to register hundreds of Zebrafish embryo. paper describing DRMIME:
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@guijacquemet
Guillaume Jacquemet
2 years
The latest contribution from the lab: "Mapping the Localization of Proteins Within Filopodia Using FiloMap" Behind a paywall, but let me know if you want a copy.
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@guijacquemet
Guillaume Jacquemet
4 years
Our #ZeroCostDL4Mic #NetOfTheDay is pix2pix. pix2pix can be used to perform paired image-to-image translation. We used pix2pix to predict nuclei staining (green) from actin staining (magenta). Learn more here: Train pix2pix here:
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@guijacquemet
Guillaume Jacquemet
5 years
My lab website is (officially) LIVE !! any feedbacks would be appreaciated ! also there is still time to apply to the available PhD position.
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@guijacquemet
Guillaume Jacquemet
6 years
I review 5x the nb of paper I submit, Dear editors, if you want me to review more offer to your reviewers: - Travel and Research grants - Donate to charities / organisms that provide starting grants I don't care about: - being payed - discounts for submission - Reviewing points
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@guijacquemet
Guillaume Jacquemet
4 years
Delighted that our @J_Cell_Sci at a glance poster is finally out! it has been an awesome journey. I hope you find it useful. I hope that it will spur discussions on how these awesome technologies can help you make discoveries in the future.
@christlet
Christophe Leterrier
4 years
๐ŸŽบ It's here ๐ŸŽบ Grab "The cell biologist's guide to super-resolution microscopy" poster (with tons of images) and article now at @j_cell_sci ! So great to work with @guijacquemet , @alexcarisey , @hellyeh_h and @HenriquesLab to make this ๐Ÿ™
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Guillaume Jacquemet
2 years
@sujanghm has now set up expansion microscopy (10x) in the lab! Spinning disk imaging of a dividing cell. Samples also look great using #eSRRF and Airyscan. Video credit to Sujan Ghimire
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@guijacquemet
Guillaume Jacquemet
3 years
๐Ÿšจ I am urgently looking for a motivated student to join my lab to do a Ph.D., as our top candidate dropped out ๐Ÿ˜”. Joint Ph.D. with Aubin Penna to work on mechanosensitive calcium channel in melanoma. Contact me ASAP if interested. Please RT ๐Ÿ™
@guijacquemet
Guillaume Jacquemet
3 years
#min รคtutkin At @BioscienceTurku @AboAkademi , my lab study how cancer cells move to block cancer metastasis. We also develop technologies and train the next generation of scientists Without funding from @SuomenAkatemia @SigridJuselius @Syopajarjestot we would not be able to work
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@guijacquemet
Guillaume Jacquemet
5 years
Would anyone be interested in a pipeline to automatically track nucleus using only free and open source software ? is this something we should write as a protocol ?
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@guijacquemet
Guillaume Jacquemet
4 years
Latest paper from @IvaskaLab and my own. "Myosin-X is required for integrin activation at filopodia tips" Work spearheaded by @mitroe with contributions from @GronlohMax and Helena Great collaboration with @bengoult @EJokitalo !
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@guijacquemet
Guillaume Jacquemet
3 years
Manuscript in preparation. Most of the plots were done using @joachimgoedhart apps :) Big fan
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@guijacquemet
Guillaume Jacquemet
2 years
My personal opinion is that ERC gives too much money to too few people. The concept of star scientists is overrated. Instead, it would be much more productive to support a much larger crowd.
@christlet
Christophe Leterrier
2 years
Am I frustrated that my career has been too slow to be competitive for an ERC grant so far? Yes. Do I think I should have been in the competition for a starting grant until last year, and be in for a consolidator until 2029? Not really. 2/2
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@guijacquemet
Guillaume Jacquemet
2 years
We have a brand new lab website ๐Ÿ˜ Still tweaking things so feedbacks are more than welcome ๐Ÿš€
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@guijacquemet
Guillaume Jacquemet
2 years
Stunning ๐Ÿคฉ
@TyskaLabActual
Matt Tyska
2 years
Epithelial cell division ๐Ÿคฉ ๐Ÿ”ฌ: @CarolineCencer @VUCellImaging @VanderbiltCDB
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@guijacquemet
Guillaume Jacquemet
7 years
I study how cancer cells move and develop drugs to stop it #showusyourscience #marchforscience
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@guijacquemet
Guillaume Jacquemet
5 years
Wow never seen anything like this ! MBoC @MBoCjournal (Molecular Biology of the Cell) Twitter Summer Fellowship : Do you love Twitter, Do you love science ? this may be for you
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@guijacquemet
Guillaume Jacquemet
7 months
I finally created a GitHub page to list the datasets we've shared in our research over the past few years. It took longer than anticipated to find all the links #opendata
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@guijacquemet
Guillaume Jacquemet
6 years
My new desktop. Simply actin.
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@guijacquemet
Guillaume Jacquemet
2 years
From recent experience, I am very impressed by the quality of service provided by @J_Cell_Sci . Great publishing experience. And the @Co_Biologists is organizing fabulous workshops and conferences. And they support @focalplane_jcs What else do we need?
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@guijacquemet
Guillaume Jacquemet
1 year
My lab at @AboAkademi turned 4 yesterday. Despite a pandemic and setbacks, it's been fun (). #Lovemyjob Big thanks to our lab members, collaborators, colleagues, and funders.
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@guijacquemet
Guillaume Jacquemet
11 months
Imaging people assemble! Send us some cool papers! Very excited by this new adventure.
@J_Cell_Sci
J Cell Science
11 months
We are pleased to announce our next special issue on imaging cell architecture and dynamics, which will be guest edited by Lucy Collinson @EM_STP and Guillaume Jacquemet @guijacquemet . Find out more: #JCSImagingSI
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@guijacquemet
Guillaume Jacquemet
6 years
This @J_Cell_Sci At a glance on "Reconstitution of cell migration " is absolutely stunning
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@guijacquemet
Guillaume Jacquemet
9 months
Interested in (cell) tracking data analysis? #CellTracksColab is slowly growing with 4 new notebooks including, a viewer to share your datasets, alternative loading strategies, and spatial analyses (clustering and distance to ROI). More to come
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Guillaume Jacquemet
4 years
Wow big thanks to the Finnish Foundation for Cardiovascular Research for supporting our work!!! ๐Ÿ™๐Ÿ™
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@guijacquemet
Guillaume Jacquemet
4 years
This paper is a light microscopy masterpiece!
@JCellBiol
JCellBiol
4 years
Researchers @KingsCollegeLon show that the #formin FMNL2 regulates the dynamics of fascin in #filopodia , thereby controlling cell-matrix sensing and potentially influencing the transition from static to migrating #cancer #cells #actin #cytoskeleton
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Guillaume Jacquemet
8 years
Currently adapting our @FijiSc software for filopodia detection #FiloQuant for live cell imaging data, getting there !
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@guijacquemet
Guillaume Jacquemet
7 years
Going to #ASCBEMBO17 ? Come and have a chat about #Filopodia Monday, Poster Board Number: B684
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@guijacquemet
Guillaume Jacquemet
2 years
๐Ÿšจ Did you know that one of the best #TrackMate features was released very quietly by @jytinevez ? You can now do BATCH analyses WITHOUT scripting in TrackMate. Just select "TrackMate Batcher" ๐Ÿ”ฌ๐Ÿš€
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Guillaume Jacquemet
3 years
Getting ready for my talk!
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Guillaume Jacquemet
4 years
New #ZeroCostDL4Mic notebook available: This time Cellpose!
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@guijacquemet
Guillaume Jacquemet
5 years
PhD position available in my lab @aboakademi @BioscienceTurku ! To investigate the molecular mechanisms by which filopodia sense environmental cues and how the information is relayed to the cell body. Please RT and share !
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@guijacquemet
Guillaume Jacquemet
5 years
As part as our advanced microscopy course, our students imaged Zebrafish embryo using our spinning disk microscope. Great lectures by @MarkkuASaari (SDCM) and Ilkka Paatero (Zebrafish). Thanks Ilkka for the samples (red are red blood cells) @BioscienceTurku @turkubioimaging
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@guijacquemet
Guillaume Jacquemet
2 years
#ELMI2022 is now over. We did it!! With over 525 participants. Thanks everyone for joining / tunning in. If you are traveling back home, Have a safe trip.
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Guillaume Jacquemet
7 years
Late #FocaladhesionFriday picture acquired on our @GEHealthcare OMX
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@guijacquemet
Guillaume Jacquemet
3 years
Hey #TrackMate @FijiSc users, did you see that new TrackMate update sites are now available ๐Ÿ˜„. Some really cool new features are being rolled out by @jytinevez . Watch out for more info.
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@guijacquemet
Guillaume Jacquemet
6 years
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