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Varun Venkataramani Profile
Varun Venkataramani

@VarunVenkatara2

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Brain Tumor Researcher, Neurologist and Neuroscientist @uniklinik_hd #PhysicianScientist #CancerNeuroscience #DigitalHealth #AI #MethodsDevelopment

Heidelberg, Germany
Joined September 2019
Don't wanna be here? Send us removal request.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
6 months
🔬✨ Just in time for #FluorescenceFriday ! Thrilled to share our preprint on an approach to battle brain tumors! How can we understand & target the cellular networks between neurons and yet incurable brain tumors? Dive into rabies-based retrograde tracing of glioblastoma! 🧠💡
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Very happy to share our latest manuscript @CellCellPress with you: TL;DR: Glioblastomas are incurable brain tumors colonizing the entire brain. We overlay molecular and functional data to uncover a hijacking of neuronal mechanisms driving invasion.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
1/ We're thrilled to share our preprint on Deep3P - a new approach that takes us on a deep dive into the world of brain tumors, using three-photon microscopy and #AI . This exciting endeavor is brought to life together with the amazing @Prevedel_Lab !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
It is our privilege that @Nature has picked our manuscript as Research Highlight 👉. Thanks so much !
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Cancer cells hijack nerve cells to storm through the brain

Nature - Cells of the deadly tumour glioblastoma hasten their advance by turning neurons to their advantage.

www.nature.com
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Very happy to share out latest #CancerNeuroscience review about multicellular brain tumor networks in diffuse glioma led by @YvoYang @MarcCSchubert together with T. Kuner, @wick_wolfgang @Winkler_Lab : @uniklinik_hd @DKFZ @CancerNeurosci1 @HdNeurooncology !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Very honoured to have our work highlighted by the great @michelle_monje and @Kathryn_RTaylor at @CellCellPress () ! Thanks so much ! The metaphor of malignant pioneers exploring the brain is really great and distills our observations from in vivo imaging.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
7 months
Thrilled to receive the #BIALAwardinBiomedicine 2023🏆! This achievement is a a testament to the hard work and dedication of all collaborators and our supportive institutions. Special thanks to the teams at @UniHeidelberg , @DKFZ , @uniklinik_hd , and our partners globally. Our
@bialfoundation
BIAL Foundation
@bialfoundation
7 months
🏆And the winning paper of the #BIALAwardinBiomedicine 2023 edition is “Glutamatergic synaptic input to glioma cells drives brain tumour progression", published in 2019 in @Nature , co-authored by 29 international researchers, led by @VarunVenkatara2 , Frank Winkler- @Winkler_Lab ,
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Very happy that our latest Perspective is now online @NatureRevCancer ! We discuss implications of malignant, multicellular networks and potential therapeutic strategies how to disconnect them. Thanks to everyone involved ! #CancerNeuroscience #TumorMicrotubes #neurotwitter
@NatureRevCancer
Nature Reviews Cancer
@NatureRevCancer
2 years
NEW content online! Disconnecting multicellular networks in brain tumours
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Amazing lecture by @michelle_monje receiving the Victor Levin Award @NeuroOnc ! Congratulations ! Thank you for all your contributions to #CancerNeuroscience !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
10 months
🚀 The @VarunVenkatara2 lab is excited to be at #SNO2023 in Vancouver! @StellaSoyka will unveil our latest in deep intravital three-photon imaging of brain tumors and their microenvironment, correlative MRI and more, Saturday 8:05 AM in Room 118-120. 🌟 🤝 Delighted to co-chair
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Varun Venkataramani
@VarunVenkatara2
10 months
Amazing #CancerNeuroscience session this morning at #SNO2023 ! Really proud of @StellaSoyka for presenting our work on deep intravital imaging of brain tumors @Prevedel_Lab ! Exciting times ahead for this emerging field!
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Following brain tumor networks over weeks with in-vivo two-photon microscopy 🧠🔬 Glioblastoma cells in green, SR101 as tumoral connectivity marker in red #FluorescenceFriday #CancerNeuroscience #BrainTumorNetworks #DisconnectTumors @YvoYang @slava__bobrov
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
8 months
Great collaborative #CancerNeuroscience project! Thanks so much to everyone involved!
@MA_Karreman
Matthia Karreman
@MA_Karreman
8 months
We are exited to share our discovery of excitatory synapses between neurons and tumor cells in brain metastases of breast cancer & melanoma. The groundbreaking study co-led by @VarunVenkatara2 and me, with Thomas Kuner & @WinklerLab : #CancerNeuroscience
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
Amazing collaboration with @Prevedel_Lab to further develop tools for Cancer Neuroscience ! You can read here more about this project:
@valientelab
Manuel Valiente
@valientelab
1 year
Not satisfied with the amazing EM images showing that invading glioma cells form synapses with neurons, @VarunVenkatara2 is developing three-photon microscopy to go deeper and reach these cells invading the corpus callosum. #EANO2023
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
3 years
Amazing cancer neuroscience Session with inspiring talks @Winkler_Lab @michelle_monje @HerveyJumper @wick_wolfgang ! Exciting times ahead! #CancerNeuroscience
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Please RT. Come work with Anna Kreshuk @ilastik_team and me at the intersection of AI, neuroscience and oncology to advance #CancerNeuroscience ! Check out our latest manuscripts @CellCellPress () and @NatureRevCancer ()
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@AIHCluster
AI Health Innovation Cluster
@AIHCluster
2 years
Help us to figure out how brain tumors ‘think‘ ! We study multicellular cancer networks with a combination of in vivo imaging, machine learning and artificial intelligence algorithms @embl @DKFZ @uniklinik_hd ! @ilastik_team @VarunVenkatara2
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
10 months
We had a wonderful time in Vancouver at a fantastic #SNO2023 ! Great science, a beautiful city and a lot of fun! Thanks to the organizers @NeuroOnc and @michelle_monje @humsav @HerveyJumper for hosting this amazing event!
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
🎆Lighting up your #FluorescenceFriday and unmasking the invisible enemy within🧠💡. Our latest video shows how brain tumor cells craft cunning networks within white matter tracts to 'hide' in plain sight. Curious for more? Dive into our Deep3P preprint below! #CancerNeuroscience
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
1/ We're thrilled to share our preprint on Deep3P - a new approach that takes us on a deep dive into the world of brain tumors, using three-photon microscopy and #AI . This exciting endeavor is brought to life together with the amazing @Prevedel_Lab !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
3 years
Terrific talk by the amazing @michelle_monje and great discussion afterwards in the 'Cancer Neuroscience Seminar Series'. It was an honour to have you here ! Looking forward to the next developments of this exciting new field !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Looking forward to a great day of #CancerNeuroscience with a lot of exciting talks @NeuroOnc at the Education Day !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
It was a pleasure to write a commentary () for @trendscancer about the recently published manuscript from Ben Deneen's lab in Neuron () ! Exciting times to study neuron-tumor networks ! #CancerNeuroscience
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Hirntumorzellen erobern das Gehirn als neuronale Trittbrettfahrer @uniklinik_hd @UniHeidelberg @DKFZ
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Outstanding talk by Antonio Iavarone about glioblastoma cell states and #CancerNeuroscience ! @EANOassociation
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@CellPressNews @CellCellPress @HeidelbergU The evolution of brain tumor networks. Learn more about the 'sociology' of brain tumors. See here how the brain is invaded by pioneering glioma cells to evolve into an interconnected network. More soon !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Fantastic #CancerNeuroscience talk by @humsav @venkatesh_lab with a lively discussion by the audience ! Looking forward to the development of this exciting field !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Thanks @NatureRevCancer for the great summary of our work ! Check out our recent manuscript @CellCellPress () and our Perspective article @NatureRevCancer () to learn more about multicellular tumor networks !
Disconnecting multicellular networks in brain tumours

Nature Reviews Cancer - This Perspective discusses the role of multicellular tumour networks, formed by tumour microtubes and tunnelling nanotubes, in brain tumours. It also discusses their...

www.nature.com
@NatureRevCancer
Nature Reviews Cancer
@NatureRevCancer
2 years
NEW content online! Impersonating neurons
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Very grateful that our Perspective article could inspire this awesome cover ! 😀How can we disconnect multicellular brain tumor networks and how can this be translated to the clinic ? #CancerNeuroscience #NeuronGliomaSynapses @wick_wolfgang @Winkler_Lab @CancerNeurosci1
@NatureRevCancer
Nature Reviews Cancer
@NatureRevCancer
2 years
Our Aug issue, out NOW! With articles on nonsense-mediated RNA decay, diet interventions as #therapy , #polyamines & disconnecting multicellular networks in brain #tumours ! Plus, a World View on what cancer survivors can teach researchers. 💌 @natrescancer
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
9 months
🧠 Exciting news from our latest research published in @JAMANetworkOpen ! We've explored the performance of Large Language Models (LLMs) in neurology board-style exams. The results? Fascinating implications for AI in healthcare and neurology in specific:
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Performance of Large Language Models on a Neurology Board–Style Examination

This cross-sectional study assesses the performance of large language models (LLMs) on neurology board–style examinations.

jamanetwork.com
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
I would like to thank everyone involved in this work which could not have been possible without the joint enormous efforts by everyone involved ! It was a great journey and I am looking forward to the next steps ! (24/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@NatureRevCancer The power of intravital imaging: Communicating brain tumor networks as seen with in vivo two-photon 3D calcium imaging 😃 But it is even more complex ! More soon !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@EricTopol @CellCellPress @Franklandlab Thanks for the shoutout ! It was a great honour to summarize and discuss the great work by the labs of @michelle_monje @VirusesImmunity !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
3 years
It was an honour to co-chair with Susan Chang @NeurosurgUCSF the "Studies that changed concepts" session @EANOassociation with excellent talks from Ingo Mellinghoff @sloan_kettering , Carmen Balana from the Catalan Institute of Oncology and @FarshadNassiri @UofTNeuroSurge
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How are neuronal mechanisms hijacked by glioblastoma cells to promote invasion ? Find here a🧵of our latest paper @CellCellPress :
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Amazing piece by @Nature ! Really excited to be working on deep imaging technologies with @Prevedel_Lab to advance #CancerNeuroscience !
@HenriquesLab
Ricardo Henriques
@HenriquesLab
2 years
🔬🧠😎 Excellent technology feature highlighting the new movement towards #SmartMicroscopes for #Biology . Including work by us and many other outstanding laboratories such as @IlariaTesta4 , @Prevedel_Lab , Pardo-López and Keller. Check it out!
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@CellPressNews @CellCellPress @HeidelbergU The manuscript is not yet online but stay tuned ! 😀
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Just in time for #fluorescencefriday : Glioblastoma cells in vivo at the invasive edge, segmented and postprocessed with an oilpaint filter. Malignant pioneers ( @michelle_monje @Kathryn_RTaylor ) drive glioblastoma invasion. For more, see @CellCellPress
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Great talk from @ggargiul_2020 discussing cell state transitons in glioblastoma ! @EANOassociation
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Brain tumor cells crawling through the brain hijacking neuronal-like mechanisms. For more:
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Great #CancerNeuroscience talk by David Nathanson ! @NathansonLab @UCLA Highly interesting data on Brain Cancer Neuroscience, Apoptosis and Therapeutic Resistance !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@CellPressNews @CellCellPress @HeidelbergU Very happy that the manuscript is now online here: Finally online: A thread will follow soon !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Thanks so much for this great summary of our work @CD_AACR !
@CD_AACR
Cancer Discovery
@CD_AACR
2 years
Read this week's Cancer Discovery #ResearchWatch , Neuronal Mechanisms Drive Whole Brain Invasion in #Glioblastoma , a summary of the paper by @VarunVenkatara2 , Thomas Kuner, @Winkler_Lab et al. @uniklinik_hd @DKFZ @HdNeurooncology @CancerNeurosci1 @NCT_HD
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
It is very exciting to see that recent work by @roelverhaak and @fsvarn could confirm that neuronal signaling was indeed associated with a more invasive phenotype of human glioblastoma upon recurrence () (21/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Great talk by @TiroshLab about the interaction of glioblastoma cell state heterogeneity and the immune system ! @EANOassociation
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Varun Venkataramani
@VarunVenkatara2
2 years
Brain tumor cells invade the brain as neuronal free riders
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Brain tumor cells invade the brain as neuronal free riders

Scientists at Heidelberg University Hospital, Heidelberg University and the German Cancer Research Center have discovered an elementary new spreading strategy in gioblastomas, the most aggressive of...

www.klinikum.uni-heidelberg.de
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Hirntumorzellen erobern das Gehirn als neuronale Trittbrettfahrer @uniklinik_hd @UniHeidelberg @DKFZ
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Varun Venkataramani
@VarunVenkatara2
2 years
Highly interesting #CancerNeuroscience work further illustrating the important role of the nervous system in the tumor microenvironment and even influencing immunosurveillance !
@BarbMarte
Barbara Marte
@BarbMarte
2 years
new out in Nature:
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Varun Venkataramani
@VarunVenkatara2
1 year
7/We also found two new vascular invasion route within the densely packed corpus callosum ! Glioblastoma cells can extend their neurite-like processes to establish a connection to blood vessels, and subsequently, relocate their cell bodies closer to these vessels.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@CellPressNews @CellCellPress @HeidelbergU TL;DR: Glioblastoma hijacks neuronal mechanisms for invasion. We find three neuronal layers: a molecular neuronal-like cell state driving glioblastoma invasion, neuronal-like cellular mechanisms and neuron-glioma synapses driving neurite-like sprouting of tumor microtubes.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Amazing ! #tumormicrotubes #tumornetworks #cancerneuroscience
@CraigHorbinski
Craig Horbinski
@CraigHorbinski
2 years
Spectacular example of an IDH1 R132H immunostain lighting up #astrocytoma cell with numerous cell processes. #pathology #neuropath #PathTwitter
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Varun Venkataramani
@VarunVenkatara2
2 years
8000x expansion and EM-like imaging with transmitted light microscopy ! Amazing @bewersdorflab !
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@biorxivpreprint
bioRxiv
@biorxivpreprint
2 years
Unclearing Microscopy #bioRxiv
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Varun Venkataramani
@VarunVenkatara2
2 years
@CellPressNews @CellCellPress @HeidelbergU Very happy that the manuscript is now online here: A thread will follow soon !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
3/So far, we could only investigate the behavior of tumor cells in the superficial layers of the cortex using two-photon microscopy. Now, we venture into the depths of the corpus callosum to study glioblastoma biology.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
6 months
Congrats, Stefan and team!!!
@ElizSMcKenna
Elizabeth McKenna
@ElizSMcKenna
6 months
The fourth winning @CancerGrand team is a second (!) team for the Solid Tumors in Children challenge: Team PROTECT, led by Stefan Pfister ( @KiTZ_HD ) - congratulations!
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Varun Venkataramani
@VarunVenkatara2
2 years
Looking forward to talk about the clinical implications of brain cancer neuroscience with the fantastic @michelle_monje and Ben Deneen !
@noa_germany
NOA
@noa_germany
2 years
🚨 Don`t miss our next webinar on brain cancer neuroscience on February 2, starting at 4.30 pm (CET). Find the dial-in link and more details on ynoa
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Varun Venkataramani
@VarunVenkatara2
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2/ Spearheaded by the impressive team @MarcCSchubert , @StellaSoyka , and @AmrTamimi9 , we're pushing the technological boundaries of #CancerNeuroscience .
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Previously, we saw a subpopulation of glioblastoma cells was connected with each other via gap junctions while other tumor cells appeared unconnected . The tumor-tumor network was resistant toward therapy. But what is the role of the seemingly unconnected tumor cells ? (2/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
5 months
Really exciting, collaborative project with @florianjug @ashesh0 @ekinreyhann ! Looking forward to push the boundaries on this important project for #CancerNeuroscience !
@ekinreyhann
Ekin Reyhan
@ekinreyhann
5 months
Spent a productive and very fun week with the @jug_lab at the Human Technopole in Milan!Many thanks to @florianjug for hosting me and especially @ashesh0 for helping me move further with our project😊Can’t wait to share results of our collaborative efforts soon! @VarunVenkatara2
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Varun Venkataramani
@VarunVenkatara2
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Surprisingly, we found that some tumor cells not only talk to each other but also with astrocytes via gap junctions while the unconnected cells were neither connected to tumor cells nor astrocytes. (4/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
A thread will follow shortly ! Thanks to everybody involved in this project on and off Twitter ! It was a huge collective effort all over the globe. @MarcCSchubert @ekinreyhann @SvenjaKTetzlaff @nikwiss @StellaSoyka @RPramatarov @Rb409Wagner @wick_wolfgang
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Varun Venkataramani
@VarunVenkatara2
2 years
Images taken and postprocessed by the stellar @StellaSoyka @MarcCSchubert @YvoYang @Rb409Wagner @RPramatarov @ekinreyhann 😃 Check out the excellent preview by @michelle_monje @Kathryn_RTaylor @CellCellPress here:
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Invasive glioma cells: The malignant pioneers that follow the current

Glioblastoma is a lethal, diffusely invasive brain cancer that is robustly regulated by the activity of the brain itself, in part through neuron-to-glioma synaptic communication. Venkataramani et al....

www.cell.com
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Using in vivo timelapse imaging in xenograft models, we could identify tumor cells not connected to astrocytes and other tumor cells as drivers of glioblastoma invasion. (5/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
To characterize molecular and functional glioblastoma cell states we used a combination of in vivo imaging and single cell RNA-sequencing with the dye SR101 that could distinguish tumor-connected and and tumor-unconnected glioblastoma cells. (3/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
4/Utilizing a powerful trifecta of three-photon microscopy, adaptive optics for high image resolution, and AI for denoising, we've journeyed further into glioblastoma. We've attained an unprecedented 1.2 mm imaging depth to peer into brain tumor biology with high resolution.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@YvoYang @MarcCSchubert @ekinreyhann @SvenjaKTetzlaff @nikwiss @StellaSoyka @RPramatarov @Rb409Wagner @wick_wolfgang @Winkler_Lab @Miriam__Ratliff @DirkCHoffmann @sahm_lab @AnGolebiewska @yahayayabo @uniklinik_hd @DKFZ @HdNeurooncology @NCT_HD @CancerNeurosci1
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
If you want to learn more have a look at our paper (), this great Preview by @michelle_monje @Kathryn_RTaylor () or this Twitter thread:
Tweet card media
Invasive glioma cells: The malignant pioneers that follow the current

Glioblastoma is a lethal, diffusely invasive brain cancer that is robustly regulated by the activity of the brain itself, in part through neuron-to-glioma synaptic communication. Venkataramani et al....

www.cell.com
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Thanks for calling attention to our work ! For more information, have a look at our 🧵:
@NikonInst
Nikon Instruments
@NikonInst
2 years
New Cell article uses #DeepLearning enabled intravital subcellular time-lapse imaging (DeepISTI) to reveal neuron-like glioblastoma behavior driving tumor invasion, with help from Nikon's Enhance_ai software to enhance signal and reduce phototoxicity:
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Our work can only be a small puzzle piece in a very exciting, emerging area of #CancerNeuroscience research. It is clear that we need to study these incurable brain tumors in a multidisciplinary fashion... (22/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Leveraging in vivo two photon microscopy with SR101 as connectivity marker we could demonstrate that tumor cell/astrocyte-unconnected glioblastoma cells evolve to tumor cell/astrocyte-connected glioblastoma cells over time. (7/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
8/They behave much like climbers ascending a mountain.The neurite-like processes act as their 'ropes', and once they have that hold, they pull their 'base camp' closer to this new foothold. Glioblastoma cells may also establish a more comprehensive interaction with blood vessels:
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
9/, attaching both their neurite-like processes and cell bodies to these vessels. In this scenario, the cells utilize the blood vessels as a type of 'perivascular highway', effectively leveraging them for mobility and dissemination.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
6/Deep3P has granted us fascinating insights. Unveiling perivascular invasion as a primary invasion pathway into the corpus callosum while glioma invasion within the cortex is only mediated to a small portion via perivascular mechanisms.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Lastly, we wanted to understand whether neuron-glioma synapses that we previously described ()also influence tumor microtube genesis and dynamics.And indeed ! Neuronal activity increased tumor microtube genesis and dynamics as well as invasion speed.(14/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@michelle_monje Thanks, Michelle ! Great collaborative work ! @YvoYang @MarcCSchubert @svenjakt @ekinreyhann @nikwiss @RPramatarov @StellaSoyka @Rb409Wagner @wick_wolfgang @DirkCHoffmann @wick_wolfgang @Winkler_Lab @uniklinik_hd @DKFZ and all coauthors not on Twitter !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
5/Our research also taps into the third-harmonic generation signal, classified using tailored machine learning. This magnifies our ability to image both the tumor and its surrounding environment of blood vessels and myelinated axonal tracts.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
Very cool work by the Lichtman lab taking vCLEM to the next level ! Congrats !
@YaronMeirovitch
Yaron Meirovitch
@YaronMeirovitch
1 year
If you are interested in the relationship between the structure and function of neurons, see this new study from our lab that correlates electron microscopy connectomics of the #cerebellum with molecular labels, using detergent-free scFv-based immunofluorescence. #VEM @Harvard 👇
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
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What molecular cell states are now associated with invasion ? Using different classifications, we see that neuronal-like and neural progenitor-like/oligodendrocyte-precursor are enriched in the glioblastoma subpopulation driving invasion. (8/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
10/Our study has also revealed the existence of intricate networks formed by glioblastoma cells, intertwined within the compact myelinated tracts of the corpus callosum, opening new avenues for further exploration.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Inhibiting calcium transients with a calcium chelator inhibited tumor microtube dynamics and reduced invasion. (16/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
11/With Deep3P, we can finally demystify why traditional MRI struggles to offer a comprehensive view of invaded areas. While changes aren't detectable with diffusion-weighted imaging, we have discovered that microscopic tractography shows no changes in myelinated tracts.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Next, we wanted to understand whether these connectivity states can be distinguished and how they evolve over time. scRNAseq showed that they can be molecularly separated and potentially transition from unconnected to connected tumor cells over time. (6/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
12/Interestingly, our research has defined an imaging biomarker of white matter disruption during early glioblastoma colonization marking an interesting starting point for clinical imaging biomarker development.
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
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Great neurooncology session today @DGNR_org with @MBreckwoldt and other great speakers ! Thanks for the opportunity to present our work on #CancerNeuroscience and #BrainTumorNetworks !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
We analyzed the scanning behaviour of tumor microtubes in more depth and found that this could be described a Levy-like movement pattern, a search-efficient mechanism that could previously also be seen in animal predators looking for scarce sources of food. (12/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Does the molecular neuronal-like cell state translate to neuronal cellular patterns of invasion ? We adapted restoration microscopy algorithms together with in-vivo two-photon imaging to follow small neurite-like processes called tumor microtubes. (9/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
3 years
@wick_wolfgang @NeuroOnc @MGHCancerCenter Great session with amazing talks about precision medicine @wick_wolfgang
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@Winkler_Lab @Miriam__Ratliff @DirkCHoffmann @sahm_lab @AnGolebiewska @uniklinik_hd @DKFZ @HdNeurooncology @NCT_HD
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Taken together, we find three neuronal layers: a molecular neuronal-like cell state driving glioblastoma invasion, neuronal-like cellular mechanisms and neuron-glioma synapses driving neurite-like sprouting of tumor microtubes. (18/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do single glioblastoma cells evolve over time on a molecular, cellular, connectivity and functional level ? How does the microenvironment change in parallel ? And very importantly: How do these results translate to the clinic ? (20/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@LaskerFDN @Winkler_Lab Thanks so much for mentioning our work. For more, have a look at our thread 🧵:
@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
How do glioblastoma invade and colonize the entire brain ? Overlaying molecular, structural and functional data helped us to uncover three layers of neuronal mechanisms driving glioblastoma invasion (). A thread 🧵: (1/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
15/ Many thanks to @MarcCSchubert , @StellaSoyka , @AmrTamimi9 ,Emanuel Maus, Robert Denninger, @nikwiss , @ekinreyhann , @SvenjaKTetzlaff , Carlo Beretta, @MichaelDrumm15 Julian Schroers, Alicia Steffens, Jordain Walshon, Kathleen McCortney, Sabine Heiland, @AnGolebiewska ,Felix Kurz,
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
16/ @wick_wolfgang @Winkler_Lab , @ilastik_team , Thomas Kuner, @CraigHorbinsk !!!
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
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...with further preclinical and clinical-translational work to push this field forward. (23/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Observation with in vivo imaging revealed that three overall movement patterns could describe the invasion pattern, reminiscent of neuronal migration patterns during development: branching migration, locomotion and translocation. (13/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
3 years
@michelle_monje @CureSearch @StBaldricks @SU2C @theNCI @NINDSnews @NIH_CommonFund @DefeatDIPG @chadtough @parkerici @Ludwig_Cancer @TheVFoundation @CIRMnews @AlexsLemonade @awoccf @Stormtheheavenz Great work as always @michelle_monje and team! Congratulations to everyone involved ! Looking forward to reading this manuscript !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Genetic perturbation and pharmacological AMPA receptor inhibition led to reduced reduced tumor microtubes per cell, reduced branching points and overall reduced tumor microtube length. (17/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
1 year
13/We owe a tremendous debt of gratitude to our interdisciplinary team at @uniklinik_hd , @UniHeidelberg , @EMBL , @DKFZ , @ResearchLifesci , @CancerNeurosci1 and across the world !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
@AnGolebiewska @Winkler_Lab @LIH_Luxembourg @yahayayabo Thanks, Anna ! Was a great collaboration !
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Using this approach, we found three mechanisms govering tumor microtube dynamics similar to neurites during path-finding: branching, protrusion and retraction. (10/24)
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Investigating the mechanisms with 3D in vivo calcium imaging, we found that glioblastoma cells not only show calcium waves and whole-cell calcium transients but also calcium microdomains. (15/ )
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@VarunVenkatara2
Varun Venkataramani
@VarunVenkatara2
2 years
Exciting work by @Loom19321 and @DavidEClapham ! Serotoniergic axon-cilium synapses are discovered and extensively characterised. Where else can these non-canonical synapses be found and what are their roles in (patho)physiological contexts ?
@Loom19321
Shu-Hsien Sheu
@Loom19321
2 years
Happy to share that our work on axon-cilium synapse is now published at Cell😃
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