Stefan W. Hell @Stefan_W_Hell Twitter profile

Last Seen Profiles

@usoksy

@ParsifalTheKing

@Grant13664399

@snephew25

@sunnyrainflow

@shiitcoiinpapii

@icerivereu

@jeotng

@0oWatches

@EB47452

@vinsmokeGu

@zhngchn34190086

@draevans

@nande_julian

@bokeplokalmalam

@therewasad

@FNCompetitive

@Sudamericana

@CanISayRetarded

@W0NIESBEAR

@goku4983

@Dctodave

@songpapdi

@shiyashimi

@StreamexVip

@rel8media

@Dannydofutebol

@Aaron_Rackley

@RMCunliffe

@hilianaaraquel

@Natalia15755315

@cody_nichols16

@huyaYangMM

@Mendaur30

@Degen__Protocol

@Reniq15

Stefan W. Hell

@Stefan_W_Hell

1 year

Huge congratulations Katalin! So deserved for the live-saving work that hardly anyone expected a decade ago. Creativity + perseverance is a recipe for real breakthroughs and #NobelPrize ⁦ @kkariko ⁩

23

125

2K

Stefan W. Hell

@Stefan_W_Hell

2 years

Folks, let’s open up a new chapter in the quantification of protein movements and conformational changes!

7

71

428

Stefan W. Hell

@Stefan_W_Hell

1 year

Powerful microscope captures motor proteins in unprecedented detail

Powerful microscope captures motor proteins in unprecedented detail

Nature - Called MINFLUX, the super-resolution method allows researchers to track molecules under cellular conditions.

www.nature.com

1

97

378

Stefan W. Hell

@Stefan_W_Hell

3 years

MINSTED goes Ångström 😎

MINSTED nanoscopy enters the Ångström localization range

We report all-optical, room-temperature localization of fluorophores with precision in the Ångström range. These precisions are attained in a STED microscope, by encircling the fluorophore with the...

www.biorxiv.org

2

91

370

Stefan W. Hell

@Stefan_W_Hell

2 years

#STED at Å precision! See New article by Weber, von der Emde et al. Fantastic applications ahead; stay tuned! #Nanoscopy #Superresolution #Microscopy @mpi_nat

4

66

319

Stefan W. Hell

@Stefan_W_Hell

4 years

Superresolution fluorescence microscopy with molecule-size resolution now walks on two feet: see our preprint on MINSTED nanoscopy by Weber, Leutenegger et al

0

94

261

Stefan W. Hell

@Stefan_W_Hell

1 year

Fretting over #FRET distances 😥? #MINFLUX can be the answer 😃💡! See our paper by S. Sahl et al:

Direct optical measurement of intra-molecular distances down to the Ångström scale

Optical investigations of nanometer distances between proteins, their subunits, or other biomolecules have been the exclusive prerogative of Förster Resonance Energy Transfer (FRET) microscopy for...

www.biorxiv.org

1

76

253

Stefan W. Hell

@Stefan_W_Hell

10 months

Happy holidays and a good resolution for 2024 🔬😀!

The Nobel Prize

@NobelPrize

10 months

"It's not a goal that is the goal, it's actually the path towards the goal." 2014 chemistry laureate Stefan Hell shares his best career advice for young scientists and researchers. #NobelPrize

18

108

451

2

18

179

Stefan W. Hell

@Stefan_W_Hell

7 months

You think you know how kinesin walks on microtubules - after all, you know the famous cartoon… #MINFLUX now forces us to think differently.

Dual-color MINFLUX: Kinesin-1 takes Chassé-Inchworm steps

Despite tremendous efforts using various techniques, many central questions regarding the walking mechanism of the ATP-driven motor protein kinesin-1 remained contradictory and puzzling. Still, it is...

www.biorxiv.org

1

38

172

Stefan W. Hell

@Stefan_W_Hell

2 years

Enhanced incorporation of subnanometer tags into cellular proteins for fluorescence nanoscopy via optimized genetic code expansion | Proceedings of the National Academy of Sciences

Enhanced incorporation of subnanometer tags into cellular proteins for fluorescence nanoscopy via...

With few-nanometer resolution recently achieved by a new generation of fluorescence nanoscopes (MINFLUX and MINSTED), the size of the tags used to ...

www.pnas.org

1

33

159

Stefan W. Hell

@Stefan_W_Hell

1 year

The roaring twenties of microscopy have just begun…

Physics Today

@PhysicsToday

1 year

Building on Nobel-winning research into optical superresolution microscopy, researchers have now achieved nanometer and millisecond precision simultaneously—good enough to watch the motions of proteins in real time.

1

15

63

0

19

137

Stefan W. Hell

@Stefan_W_Hell

1 year

MINSTED is on the fast track 🤓

MINSTED tracking of single biomolecules

We show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecu...

www.biorxiv.org

1

24

119

Stefan W. Hell

@Stefan_W_Hell

1 year

Interacting with so many bright young minds is a real privilege and highlight for me, every year.

Daniel Rosenblum

@DanielRosePhD

1 year

Mind-blowing talk by @Stefan_W_Hell , bringing fluorescent microscopy to Angstrom resolutions. #LINO23 @lindaunobel

0

7

39

0

4

119

Stefan W. Hell

@Stefan_W_Hell

7 months

#STED shows a new and unexpected facet of its capabilities: quick, effective and precise fluorophore tracking. #MINFLUX . Good work Henrik, Lukas, Michael, Mira...

Nature Methods

@naturemethods

7 months

Out today from @StefanHellLabs MINSTED enables single-particle tracking with nanometer/millisecond spatiotemporal precision.

0

20

61

1

19

116

Stefan W. Hell

@Stefan_W_Hell

2 years

Relaxing after a series of spectacular lectures on the very latest developments in astronomy, biology, mathematics and chemistry at ⁦ @CasinaPioIV ⁩. What a day!

1

4

115

Stefan W. Hell

@Stefan_W_Hell

1 year

Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy | ACS Central Science

Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy

Here we describe highly compact, click compatible, and photoactivatable dyes for super-resolution fluorescence microscopy (nanoscopy). By combining the photoactivatable xanthone (PaX) core with a...

pubs.acs.org

0

26

106

Stefan W. Hell

@Stefan_W_Hell

3 years

A place that makes you think and feel humble: Albert Einstein's summer home in #Caputh near Berlin, where he spent three happy years of his life before his life and that of millions of others were struck by terrible events.

0

3

103

Stefan W. Hell

@Stefan_W_Hell

1 year

Three-dimensional single-cell transcriptome imaging of thick tissues

Multiplexed error-robust fluorescence in-situ hybridization (MERFISH) allows genome-scale imaging of RNAs in individual cells in intact tissues. To date, MERFISH has been applied to image thin tissue...

www.biorxiv.org

1

24

99

Stefan W. Hell

@Stefan_W_Hell

1 year

It’s wonderful to see so many successful alumni and current lab members driving the field forward and making an enormous impact. I am very proud of all of you!

Richard Lincoln, Ph.D.

@RLincolnChemist

1 year

The @StefanHellLabs family at #SMLMS2023 — 🍩's past and present united! @AndImaging , R. Gerasimaite, @IlariaTesta4 @LOstersehlt @VicidominiLab @BalzarottiFran , I.Jansen, @RLincolnChemist @ElisaDEste , J.O.Wirth, S.J.Sahl, @wmarkbates @bewersdorflab Missing: @JGDanzl & @SSchnorren

1

6

54

0

2

94

Stefan W. Hell

@Stefan_W_Hell

7 months

Photoactivatable Xanthone (PaX) Dyes Enable Quantitative, Dual Color, and Live‐Cell MINFLUX Nanoscopy - Remmel - Small Methods - Wiley Online Library

Photoactivatable Xanthone (PaX) Dyes Enable Quantitative, Dual Color, and Live‐Cell MINFLUX...

This work quantitatively explores the performance of the recently reported PaX dyes in MINFLUX imaging, comparing their localization success rate and labeling efficiency with the performance of...

onlinelibrary.wiley.com

1

17

93

Stefan W. Hell

@Stefan_W_Hell

2 years

Glad to see Thomas Ebbesen, a truly outstanding chemist and physicist with whom I shared the 2014 ⁦ @KavliPrize ⁩ in #nanoscience .

1

3

91

Stefan W. Hell

@Stefan_W_Hell

8 months

We have canceled the OFF! Unexpected super-resolution provided by a minimum #MINFLUX

Diffraction minima resolve point scatterers at tiny fractions (1/80) of the wavelength

Discerning two or more identical and constantly scattering point sources using freely propagating waves is thought to be limited by diffraction. Here we show both theoretically and experimentally...

www.biorxiv.org

2

17

89

Stefan W. Hell

@Stefan_W_Hell

3 years

3 birthdays today: twin sons turning 16 and ⁦ @Abberior ⁩ celebrating 10 years!

5

0

82

Stefan W. Hell

@Stefan_W_Hell

2 years

Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX

3D-MINFLUX nanoscopy of heat-immobilized retinal sections reveals the molecular topography of rod photoreceptor active zones.

www.science.org

0

8

78

Stefan W. Hell

@Stefan_W_Hell

4 months

🤔Guess what comes after kinesin? Dynein, of course! See our latest paper by Jonas Schleske et al #MINFLUX in living cells.

MINFLUX Reveals Dynein Stepping in Live Neurons

Dynein is the primary molecular motor responsible for retrograde intracellular transport of a variety of cargoes, performing successive nanometer-sized steps within milliseconds. Due to the limited...

www.biorxiv.org

0

20

77

Stefan W. Hell

@Stefan_W_Hell

10 months

MINFLUX is hot on the heels of kinesin in cells

Uncovering kinesin dynamics in neurites with MINFLUX

Neurons grow neurites of several tens of micrometers in length, necessitating active transport from the cell body by motor proteins. By tracking fluorophores as minimally invasive labels, MINFLUX is...

www.biorxiv.org

0

8

72

Stefan W. Hell

@Stefan_W_Hell

11 months

4Pi MINFLUX sets benchmarks in 3D tracking of fluorescence emitters. Check out:

4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter

We introduce MINFLUX localization with interferometric illumination through opposing objective lenses for maximizing the attainable precision in 3D-localization of single inelastic scatterers, such...

www.biorxiv.org

0

17

73

Stefan W. Hell

@Stefan_W_Hell

1 year

Our youngest, Wolfgang, got his t-shirt signed by the captain of his favorite soccer team VfL Wolfsburg after they beat Union Berlin 2-1 (who will play Real Madrid on Wednesday).

0

70

Stefan W. Hell

@Stefan_W_Hell

10 months

A fantastic student. I love working with her.

Physikerinnen

@physikerinnen

10 months

Katarina Chapman #PhysikerinderWoche - Katarina is a Ph.D. student in the @StefanHellLabs at the @mpi_mr_hd @maxplanckpress in Heidelberg, working on #MINFLUX #nanoscopy ... @BMBF_Bund #womeninscience #womeninphysics #physikerin 👩‍💻👉

0

8

56

1

2

69

Stefan W. Hell

@Stefan_W_Hell

10 months

Nice to see the cristae come out so clearly. 👌

Abberior

@Abberior

10 months

Exploring mitochondria dynamics with #abberior LIVE ORANGE mito! Our #STEDYCON microscope captures the live-cell magic, deconvolved and rendered with @svi_huygens . Thanks to @CrisBenincaPhD from @UCLA for the cells.

0

5

22

1

6

66

Stefan W. Hell

@Stefan_W_Hell

2 years

#MINFLUX now changes the game of protein tracking: 🌶😎🌶 MINFLUX dissects the unimpeded walking of kinesin-1 See also spectacular work by @JonasRies lab in living cell:

0

18

63

Stefan W. Hell

@Stefan_W_Hell

1 year

The pleasure was all mine! It made me happy to see you record great data and being happy with the #MINFLUX system.

Miao Yu

@MiaoooYu

1 year

I had a lovely visit to @Abberior in Heidelberg and tried my favourite protein Nup98 with Minflux! The resolution is mind-blowing!🤯🤩 I asked Clara how often they meet with Stefan Hell here. She said roughly once per month. AND then Stefan just came in! I was super excited!😇

0

2

42

1

3

65

Stefan W. Hell

@Stefan_W_Hell

1 year

So nice seeing Queen Silvia of Sweden again, on the occasion of her receiving the honorary citizenship of Heidelberg.

0

2

62

Stefan W. Hell

@Stefan_W_Hell

2 years

It has been wonderful to meet @OnsMSaad1 , formerly of the @bewersdorflab at Yale and hear about her great #panExM work!

1

59

Stefan W. Hell

@Stefan_W_Hell

10 months

I look forward to presenting at the conference!

Institut du Cerveau - Paris Brain Institute

@InstitutCerveau

10 months

We are delighted to welcome Professor Stefan W. Hell (who received the Nobel Prize in Chemistry in 2014) for a seminar about fluorescence #spectroscopy on Monday, December 11 at 11 am. 👇Join the conference via Zoom here :

2

9

13

0

4

55

Stefan W. Hell

@Stefan_W_Hell

7 months

I look forward to participating at this year’s event! Great people and great science, as always.

Lindau Nobel Laureate Meetings

@lindaunobel

7 months

Today, we invite more than 650 Young Scientists from 90+ countries to meet well above 30 Nobel Laureates at the 73rd Lindau Nobel Laureate Meeting (30 June–5 July 2024 | #LINO24 | #Physics ). We can‘t wait to welcome you all to Lindau!

4

15

126

2

3

50

Stefan W. Hell

@Stefan_W_Hell

1 year

I really enjoyed reading this fantastic study. Congratulations to Ardem, Eric and the whole team!

Ardem Patapoutian

@ardemp

1 year

Super excited about this! Check out manuscript from my amazing postdoc Eric Mulhall and collaborators measuring single molecule #PIEZO1 dimensions in cells with nanometer precision using super-resolution fluorescence microscopy: 1/9

11

113

576

0

2

42

Stefan W. Hell

@Stefan_W_Hell

2 years

Schnell auch im Nanometerbereich – Durchbruch in der hochauflösenden Fluoreszenzmikroskopie

Durchbruch in der hochauflösenden Fluoreszenzmikroskopie

Forschende können mit neuen MINFLUX-Mikroskopen Veränderungen der Proteinstruktur unter physiologischen Bedingungen beobachten

www.mpg.de

0

7

37

Stefan W. Hell

@Stefan_W_Hell

7 months

Fancy a donut? Makes you feel twice as good: when you eat it and when you see how it makes hot data…

2

34

Stefan W. Hell

@Stefan_W_Hell

6 months

Another cutting-edge application using #MINFLUX More will follow soon.

Abberior

@Abberior

6 months

Published in Cell: #NINJ1 mediates plasma membrane rupture by cutting and releasing membrane disks. #MINFLUX imaging was used to confirm ring formation of endogenous NINJ1. Great collaborative project from @WuLabHarvard @LironDavid2 @BobbyHollings

0

7

20

0

6

34

Stefan W. Hell

@Stefan_W_Hell

7 months

An ingenious combination of design, suitability for everyday use and low cost. I think it’s the nicest car that Peugeot ever built 😊…

Frédéric EGHIAIAN

@FEghiaian

7 months

Talking about France, while visiting @Abberior 's new building in Göttingen recently, I had the pleasure to learn that @Stefan_W_Hell owns a french car and likes driving them since the 1990s ! Maybe an early inspiration for studying molecular motors with #MINFLUX ?

2

1

13

1

0

22

Stefan W. Hell

@Stefan_W_Hell

7 months

Congratulations Siva! Excellent work. Also, it’s very rewarding to see that DyMin STED and MINSTED were helpful. By coincidence another MINSTED paper - on tracking- came out today in Nature Methods. I’m sure it’ll be useful, as well:

Sivakumar Sambandan

@siva_sambandan

2 years

On the @NeuroCellPress cover is our ‘Lord of the Vesicles’! Largest synaptic vesicle pool in the brain carry two #transporters , VGLUT1 and ZnT3. Very rewarding collaboration with JahnLab @Stefan_W_Hell @miloseviclab @apoliscope @mpi_nat @yourUMG !! Thanks @sci_illustrate !

1

6

41

0

11

Stefan W. Hell

@Stefan_W_Hell

1 year

@ProfMartinJBoo1 Congratulations Martin!

0

1