Hannes Schihada, PhD @HSchihada Twitter profile | Pikagi

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Hannes Schihada, PhD

@HSchihada

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Emmy Noether research group leader in #CellBiology & #Pharmacology working on #GPCRs & G proteins

Marburg, Deutschland

Joined September 2017

Don't wanna be here? Send us removal request.

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@HSchihada

Hannes Schihada, PhD

3 months

📢I have open PhD student and Postdoc positions to join my #EmmyNoether lab to decipher how #GPCR -specific signaling is achieved by heterotrimeric #Gproteins ! ‼️Interested in developing novel biosensors💡 that reveal yet invisible states and processes🔍? ➡️DM or email me📨‼️

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@HSchihada

Hannes Schihada, PhD

3 years

Delighted to share this new set of #BRET -based G protein #biosensors with the #GPCR community! Our paper's just out @scisignal and the entire toolkit is available from @Addgene ! PS: You only need to transfect a single plasmid per sensor 🧬

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Quantitative assessment of constitutive G protein–coupled receptor activity with BRET-based G...

BRET sensors that can be used to quantify the constitutive activity of GPCRs are developed.

www.science.org

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@HSchihada

Hannes Schihada, PhD

4 months

I'm extremely honored and grateful to be awarded the competitive Emmy Noether Program to establish my own research lab @Uni_MR ! I'd like to thank all my past and present mentors and colleagues for the support I've received. Let's get this started #GPCRs #Gproteins #biosensors !

@Uni_MR

Philipps-Universität

4 months

Hannes Schihada leitet eine neue Emmy-Noether Nachwuchsgruppe an der #UniMarburg , die erforscht, wie eine begrenzte Anzahl von G-Proteinen eine große Vielfalt an zellulären Reaktionen auslösen kann. Glückwunsch👏 👉 📷Markus Farnung @dfg_public @HSchihada

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@HSchihada

Hannes Schihada, PhD

1 year

We're excited to see our paper published in the Journal of Medicinal Chemistry! We developed and pharmacologically characterized the first fluorescent tool ligand for GPR3, a poorly understood orphan class A #GPCR .

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Development of Fluorescent AF64394 Analogues Enables Real-Time Binding Studies for the Orphan Class...

The orphan G protein-coupled receptor (oGPCR) GPR3 represents a potential drug target for the treatment of Alzheimer’s disease and metabolic disorders. However, the limited toolbox of pharmacological...

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@HSchihada

Hannes Schihada, PhD

2 years

Excited to see our work on GPR35 published in @jbiolchem ! We characterized two natural isoforms of the orphan #GPCR GPR35 and identified the crucial role of disulfide bridge for constitutive activity! Thanks to everyone involved @ruaslab @Laura_Humphrys !

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Isoforms of GPR35 have distinct extracellular N-termini that allosterically modify receptor-trans...

Within the intestine, the human G protein–coupled receptor (GPCR) GPR35 is involved in oncogenic signaling, bacterial infections, and inflammatory bowel disease. GPR35 is known to be expressed as two...

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@HSchihada

Hannes Schihada, PhD

3 years

I‘m thrilled and honored for receiving the @MSCActions #PostDoc fellowship! We’ll search for #orphan #GPCR ligands in the Kolb-lab @Uni_MR to find better drugs against diseases like Alzheimer‘s and Parkinson‘s. Exciting times ahead! #ToBeLi_for_GPR3_6_12 #biosensors

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@HSchihada

Hannes Schihada, PhD

2 years

Not sure how my name made it in between these three giants in the #GPCR field but I definitely don’t wanna complain 😅 Thanks to @DrGPCR for inviting me to her fabulous podcast & @dahilger for this snapshot 😉

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@HSchihada

Hannes Schihada, PhD

3 years

Leaving Stockholm 🇸🇪 today after 2.5 years of PostDoc studies @karolinskainst . Very grateful to my mentor Gunnar Schulte & all members of the @schulte_lab ! It was an exciting time with lots of scientific & personal adventures! Now looking forward to new challenges #orphan #GPCRs

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@HSchihada

Hannes Schihada, PhD

3 years

Would you like to study the conformational dynamics of your favorite #GPCR in a 96-well format? Check out our guide to develop intramolecular receptor #BRET #biosensors put together with @karantarina , Martin Lohse & Isabella Maiellaro!

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Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Na

Here we describe the stepwise application of bioluminescence resonance energy transfer (BRET)-based conformational receptor biosensors to study GPCR activation in intact cells. This technology can be...

link.springer.com

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@HSchihada

Hannes Schihada, PhD

2 years

Just signed ✍️my new contract as a @MSCActions 🇪🇺 #PostDoc at the Kolb-lab @Uni_MR ! We will seach for advanced #orphan #GPCR ligands by combining virtual screening💻of large compound libraries with HTS-amenable GPCR conformational biosensors in-vitro. Let's go & stay tuned!🤙

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@HSchihada

Hannes Schihada, PhD

2 years

BIG NEWS: @GraetzLukas & I will be a organizing a Training school on cell-based assays to study #Adhesion #GPCR function in summer 2023! This IN PERSON event will be funded by @COSTprogramme action @ca18240 ! Stay tuned for more details about Where, When & Who! #AdhernRise

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@HSchihada

Hannes Schihada, PhD

1 year

Our Training School in #Stockholm has come to an end! It's been 2 1/2 days of inspiring scientific discussions and brainstorming together with talented young researchers to lay out new strategies to develop cell-based assays for #adhesion #GPCR research! #AdhernRise @ca18240

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@HSchihada

Hannes Schihada, PhD

2 months

First data obtained with the new plate readers. Looking forward to more 🤓 #newPI

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@HSchihada

Hannes Schihada, PhD

3 years

Our latest paper from @schulte_lab is out @ScienceAdvances ! We combined #BRET with unnatural amino acid labeling to monitor conformational dynamics in the extracellular parts of #Frizzleds 🤓 Check this 🧵to find more details about our current understanding of FZD function ⬇️⬇️

@schulte_lab

Schulte lab

3 years

1/14 FZD intramolecular dynamics news - 🧵Our paper out today employs orthogonal labelling of unnatural amino acids in the extracellular domains of FZDs uncovering WNT-induced dynamics of the CRD presenting additional proof for the agonist-induced dynamics in Class F GPCRs.

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@HSchihada

Hannes Schihada, PhD

4 years

The first #Frizzled paper from my #PostDoc @schulte_lab is out! We developed #fluorescent conformational #biosensors of #FZDs to investigate their structural dynamics upon #WNT binding

@schulte_lab

Schulte lab

4 years

Excited to present our new paper on cpGFP-based #biosensors underlining that #WNTs induce conformational changes in #FZDs reminiscent of those seen in class A/B #GPCRs .. Great tools to understand WNT/FZD pharmacology! @HSchihada @DrGPCR

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@HSchihada

Hannes Schihada, PhD

2 years

Thanks @DrGPCR for having me! It was such a wonderful experience to be your guest on this wonderful podcast 🎙that I’m following since the first episode! Now looking forward to the #summit and to explore your #ecosystem to get in touch with #GPCR enthusiasts all over the 🌍

@DrGPCR

DrGPCR

2 years

🎙️ Episode 88 of the @DrGPCR podcast is here! Dr. @HSchihada is our guest and he has such an interesting experience in the field! Watch the full video with a paid @DrGPCR membership or listen wherever you get your podcast for free. ➡️ #gpcr #drgpcr

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@HSchihada

Hannes Schihada, PhD

2 years

Happy to see our work on GPR35 isoforms among the top reads of the last 30 days @jbiolchem 🍾 @schulte_lab @shamimdadvar @ruaslab @Laura_Humphrys

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@HSchihada

Hannes Schihada, PhD

2 years

Excited to see our work on GPR35 published in @jbiolchem ! We characterized two natural isoforms of the orphan #GPCR GPR35 and identified the crucial role of disulfide bridge for constitutive activity! Thanks to everyone involved @ruaslab @Laura_Humphrys !

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@HSchihada

Hannes Schihada, PhD

2 years

‼️Dates 🗓️and Trainers 👩‍🏫👨‍🏫for our @COSTprogramme Training School in #Stockholm June 28-30 are now confirmed! We're extremely happy that @LiebscherLab , @MiriamStoeber and the one and only Evi Kostenis agreed on joining @GraetzLukas and me to talk about live-cell #aGPCR assays‼️

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@HSchihada

Hannes Schihada, PhD

2 years

BIG NEWS: @GraetzLukas & I will be a organizing a Training school on cell-based assays to study #Adhesion #GPCR function in summer 2023! This IN PERSON event will be funded by @COSTprogramme action @ca18240 ! Stay tuned for more details about Where, When & Who! #AdhernRise

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@HSchihada

Hannes Schihada, PhD

2 years

Excited and looking forward to present at the @DrGPCR summit 2022 in about 30 min😃 Join us if you're interested in #GPCR conformational #biosensors and G protein probes to quantify constitutive receptor activity! The whole summit is ‼️FOR FREE‼️

@DrGPCR

DrGPCR

2 years

Don't miss our next talks tomorrow at @DrGPCR Summit @HSchihada Carole Daly, Shivani Sachdev @Gabriele_K8 Chloe Hicks @nicola_dijon @karantarina @JoanSerranoMar @KiyanAfzali Join us by becoming a @DrGPCR site member, it’s totally free! ➡️ #gpcr #drgpcr

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@HSchihada

Hannes Schihada, PhD

3 years

Just WOW! So we not only have GPCRs and arrestin-coupled receptors, but also RGS-coupled receptors?

@KLabGPCR

Kirill Martemyanov Lab

3 years

Can a #GPCR structure still surprise you? Happy to present #CryoEM structure of an orphan receptor GPR158. Couples to RGS instead of G proteins. Features an extracellular Cache domain for ligand binding Forms a unique tight dimer stapled by phospholipids

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@HSchihada

Hannes Schihada, PhD

1 month

Are you interested in #GPCR / #Gprotein signaling and #biosensor development? Then please apply for this open #PostDoc position in my new Emmy Noether Research Group @Uni_MR ! #newPI

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@HSchihada

Hannes Schihada, PhD

3 years

So excited to see this structure of a constitutively active FZD7-Gs complex online! It was a great collaboration between the labs of Fei Xu @ShanghaiTechUni , @Michel_Bouvier and Gunnar Schulte @schulte_lab !

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@HSchihada

Hannes Schihada, PhD

2 years

The tentative program for our #Training #School on cell-based #adhesion #GPCR assays is now available! Here's your link to register: ‼️‼️ @LiebscherLab @GraetzLukas @MiriamStoeber @DrGPCR @ca18240 @ErnestNetwork @sfb1423 #AdhernRise

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@HSchihada

Hannes Schihada, PhD

3 years

Excited to share our new #preprint from the @schulte_lab and @thomassakmar lab! We designed #BRET #sensors using genetic code expansion and labeling of unnatural amino acids to monitor the extracellular dynamics in #Frizzleds upon #WNT binding!

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Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced...

Frizzleds (FZD1-10) comprise a class of G protein-coupled receptors containing an extracellular cysteine-rich domain (CRD) that binds lipoglycoproteins of the Wingless/Int-1 family (WNTs). Despite...

www.biorxiv.org

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@HSchihada

Hannes Schihada, PhD

4 months

@Uni_MR We will dive into the fascinating question how so many #GPCRs expressed in the same cell can activate the same #Gprotein but induce distinct signals and cellular responses! Interested? Please reach out ✉️

How molecular specificity emerges

More than "on" and "off": German Research Foundation funds new Emmy Noether Group at Philipps-Universität

www.uni-marburg.de

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@HSchihada

Hannes Schihada, PhD

2 years

Registration opened!!!

@ca18240

CA18240 Adher´n Rise

2 years

The registration for the 4GPCRnet symposium held 26-29 September 2022 in #Leipzig is now open. Register here to not miss out on this highlight of the #GPCR year with a fantastic line-up of speakers: @ErnestNetwork @ca18240 @sfb1423

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@HSchihada

Hannes Schihada, PhD

5 months

I’m proud to become part of this fantastic #ECI seminar series! Tune in on 🗓️ June 13 ❗️to hear about our efforts in using a conformational GPR3 #biosensor to identify ligands for this orphan #GPCR .

@GPCRzoominar

GPCR ECI Zoominar

5 months

📢Book your agenda! Our next session is on June 13th (4 pm CET, 10 am EST, 7 am PST, and 11 pm ASWT) and we will host @HSchihada and Eline Pottie, two excellent ECIs working on tool development for #GPCRs . #ECI_GPCRforum

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@HSchihada

Hannes Schihada, PhD

1 year

Thanks to all the authors, especially to @SteffenPockes who led this study with me! To Merlin Bresinsky for the enormous synthesis effort and to @AidaShahraki & Peter Kolb for the invaluable insights provided by their computational approach!

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@HSchihada

Hannes Schihada, PhD

3 years

So excited to see this online!

@schulte_lab

Schulte lab

3 years

We are excited about our recent work on the dynamics of the extracellular CRD of Frizzleds in response to WNTs. First authors Maria and Hannes rock! @HSchihada @thomassakmar @simonelsasser

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@HSchihada

Hannes Schihada, PhD

4 years

Rainbow over Karolinska Institutet 🌈. A sign to kick off the weekend?

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@HSchihada

Hannes Schihada, PhD

4 years

So excited to see this online! A new #conformational sensor of the histamine H3 receptor allowed us to characterize new inverse agonists of this attractive drug target! #GPCR #BRET #sensor #inverseagonist #histamine

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Development of a Conformational Histamine H3 Receptor Biosensor for the Synchronous Screening of...

The histamine H3 receptor (H3R) represents a highly attractive drug target for the treatment of various central nervous system disorders, but the discovery of novel H3R targeting compounds relies on...

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@HSchihada

Hannes Schihada, PhD

3 years

Need to remember this trick for my next manuscript 😂 If the noise is high but you want to stress the difference between two groups, just let the error bars go different direction 😅

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@HSchihada

Hannes Schihada, PhD

2 years

@ca18240 @DrGPCR

@HSchihada

Hannes Schihada, PhD

2 years

‼️Dates 🗓️and Trainers 👩‍🏫👨‍🏫for our @COSTprogramme Training School in #Stockholm June 28-30 are now confirmed! We're extremely happy that @LiebscherLab , @MiriamStoeber and the one and only Evi Kostenis agreed on joining @GraetzLukas and me to talk about live-cell #aGPCR assays‼️

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@HSchihada

Hannes Schihada, PhD

3 years

Because they cannot be appreciated enough for paving the way for these kind of sensors already years - if not decades - ago: Big credits go to @Michel_Bouvier , @joachimgoedhart , Moritz Bünemann & Martin Lohse, Nevin Lambert, @RothLabUNC , @ReidHJOlsen and many more!

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@HSchihada

Hannes Schihada, PhD

3 years

Thanks for sharing @DrGPCR ! We hope others - also outside the #GPCR field - will find this technique useful for studying their favorite receptors as well!

@DrGPCR

DrGPCR

3 years

FZD-CRD biosensors reveal that CRD dynamics are not required for WNT/β-catenin signaling #gpcr #drgpcr Subscribe to the Dr. GPCR Newsletter Today! @schulte_lab @HSchihada @tomsakmar

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@HSchihada

Hannes Schihada, PhD

4 years

Indeed! What a nice Christmas present 🎁

@schulte_lab

Schulte lab

4 years

Paper acceptance on Christmas day is very much appreciated

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@HSchihada

Hannes Schihada, PhD

3 months

Here's the link to the first PhD student position available in my group:

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@HSchihada

Hannes Schihada, PhD

4 years

Now open access - thanks to the National Library of Sweden🇸🇪! #openaccess

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@HSchihada

Hannes Schihada, PhD

2 years

FYI: 8/10 G protein sensors ("G-CASE") used in this story are already available @Addgene ! The 2 new sensors (Gz-CASE & G12-CASE) will be deposited soon!

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@HSchihada

Hannes Schihada, PhD

3 years

Another big #GPCR meeting 🤩 Looking forward to present our data from @schulte_lab on using conformational receptor biosensors to study #Frizzled activation & signalling!

@ca18240

CA18240 Adher´n Rise

3 years

📢The next #virtual #GPCR meeting is approaching! Sign up for the iGPCRnet symposium jointly organized by the labs of Ralf Jockers, Martin Lohse & Stephen Hill ‼️Nov 30th - Dec 3rd‼️ PS: Don't miss out on session 7 covering #adhesion GPCRs!😉 #AdhernRise

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@HSchihada

Hannes Schihada, PhD

3 years

Register for the 6th @ErnestNetwork meeting 28th - 31st of March!

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@HSchihada

Hannes Schihada, PhD

3 years

Commuting to the lab in Stockholm 🇸🇪 and I’m excited to find another person wearing a face mask in the train. > 90% don’t Shouldn’t it be the opposite? I thought government recommendations are as effective as laws in this country? Pure ignorance... #COVIDinSweden

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@HSchihada

Hannes Schihada, PhD

1 year

This could be a game changer for everyone trying to develop new biosensors! Looking forward to try it out soon 🤩

@LipfertLab

LipfertLab

1 year

Do you want to label a protein (for fluorescence detection, FRET, EPR, etc.) but don’t know what residues are most suitable? Check out our “Labelizer” pipeline to predict the best labeling sites! Great collaboration with Thorben Cordes @PSB_lab 🧵 (1/n)

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@HSchihada

Hannes Schihada, PhD

3 months

Excited to participate in the Emmy Noether Meeting 2024! A beautiful symposium to hear about new DFG developments, connect with peers and learn how to manage EN projects. Thanks @dfg_public for organizing this wonderful event! #newPI

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@HSchihada

Hannes Schihada, PhD

3 years

Nice review on #FRET - and #BRET -based assays to explore #GPCR function!

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@HSchihada

Hannes Schihada, PhD

3 years

Save the Date!

@ca18240

CA18240 Adher´n Rise

3 years

📢Save the Date ‼️ @COSTprogramme actions @ErnestNetwork & @ca18240 joined forces with #GPCR #networks @sfb1423 & RU2372 to organize the #4GPCRnet International Symposium taking place in #Leipzig 🇩🇪 26th - 29th September 2022! 📢Save the Date ‼️ 🧵

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@HSchihada

Hannes Schihada, PhD

4 years

“We tested 7,800 of 7,828 possible single amino acid substitutions to the beta-2 adrenergic receptor (β2AR) at four concentrations of the agonist isoproterenol.” 😳😳😳

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Structural and functional characterization of G protein–coupled receptors with deep mutational...

Linking deep mutational scanning with engineered transcriptional reporters in human cell lines establishes a generalizable method for exploring pharmacogenomics, structure, and function across broad...

elifesciences.org

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@HSchihada

Hannes Schihada, PhD

4 years

What a nice story with beautiful techniques 😍

@ca18240

CA18240 Adher´n Rise

4 years

What a cool paper just published in @MolecularCell on the molecular mechanism of #adhesion #GPCR activation! Structural flexibility of the GAIN domain regulates tethered agonist exposure and #aGPCR activity. #Adher 'nRise #ca18240

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@HSchihada

Hannes Schihada, PhD

2 years

Check out this new study describing how #RAMPs can fine-tune #GPCR activation & signaling!

@karantarina

Katarina Nemec

2 years

My first author manuscript is finally out! Using a set of fluorescent biosensors for the parathyroid hormone 1 receptor (PTH1R) and its downstream signaling partners, we showcase specific art of GPCR modulation, that might open new ways of drug design!

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@HSchihada

Hannes Schihada, PhD

4 years

Check out this cool paper from my former collegues @aisb_0 and Jan about the disruption of #CXCR4 dimers with inverse agonists! Cool story, Bros!

Advanced fluorescence microscopy reveals disruption of dynamic CXCR4 dimerization by subpocket-sp...

Although class A G protein−coupled receptors (GPCRs) can function as monomers, many of them form dimers and oligomers, but the mechanisms and funct...

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@HSchihada

Hannes Schihada, PhD

4 years

@Amro_Hussien @DrGPCR @GPCRscientist @LiebscherLab @ProemelLab ... and about class F #GPCRs #Frizzleds with @schulte_lab 😉

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@HSchihada

Hannes Schihada, PhD

3 months

What a wonderful event! Looking forward to next year ☺️ thanks @dfg_public !

@dfg_public

DFG public | @[email protected]

3 months

Ballons, Ballons... 🎈🎈Sie schmücken viele Bilder unseres #ENT2024 ! 25 Jahre besteht das Emmy Noether- #F örderprogramm inzwischen. Ehrengast daher: Ernst-Ludwig Winnacker, der das #ENP seinerzeit als DFG-Präsident eingeführt hatte. Es waren wieder inspirierende Tage! #25JahreENP

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@HSchihada

Hannes Schihada, PhD

3 years

Only 6 more days to register 😱😱😱

@ca18240

CA18240 Adher´n Rise

3 years

Already registered for our #virtual meeting on #aGPCRs ? Don't miss the #deadline on June 13th! The preliminary programme is now available & we have a great lineup of speakers with T Langenhan, C Formstone, @demet_arac & @DrGPCR ! #AdhernRise #ca18240

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@HSchihada

Hannes Schihada, PhD

2 years

‼️Registration will close April 30th‼️ Join us in Stockholm to learn about assays to assess #adhesion #GPCR function and signaling in live cells @ErnestNetwork @ca18240 @LiebscherLab @MiriamStoeber @GraetzLukas @DrGPCR

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@HSchihada

Hannes Schihada, PhD

1 year

@marco_wenner @janboehm 💯%

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@HSchihada

Hannes Schihada, PhD

7 months

That’s a fantastic postdoc opportunity! Isabella is a very kind and great scientist!

@karantarina

Katarina Nemec

7 months

Isabella was a fantastic mentor during my PhD! Here is a postdoc opportunity in her lab! :)

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@HSchihada

Hannes Schihada, PhD

3 years

Here's the link to the @Addgene page to get the plasmids: @schulte_lab

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@HSchihada

Hannes Schihada, PhD

1 year

@ Science tweeps: which one is the fastest small GTPase? #AcademicChatter

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@HSchihada

Hannes Schihada, PhD

1 year

Thanks to all the motivated trainees and our fantastic trainers and ECI supporters! Ines Liebscher @LiebscherLab & Jakob Mitgau Evi Kostenis & Judith Alenfelder Miriam Stöber @MiriamStoeber & Arthur Radoux

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@HSchihada

Hannes Schihada, PhD

4 years

Such a cool approach! So many #peptide / #GPCR maps that could be revealed combining #CRISPR /Cas9 and #NanoLuc complementation. Congrats!

@iScience_CP

iScience journal

4 years

A Nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding

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@HSchihada

Hannes Schihada, PhD

3 years

@UCSD_NIC @joachimgoedhart @LABrechaun @NathanShaner @LoogerL It also depends a lot on the expected distance between the FRET partners in your protein:protein interaction pair or within an intramolecular sensor. The bigger the distance, the more spectral overlap between donor emission & acceptor excitation is required for a good signal.

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@HSchihada

Hannes Schihada, PhD

2 years

@DrGPCR It's been an honour to present at this wonderful summit! Thanks for putting this together @DrGPCR team!

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@HSchihada

Hannes Schihada, PhD

4 years

#GPCRs , #fluorescence #microscopy & #oligomers 🤷🏻‍♂️🤷🏽‍♀️ Check out this protocol from @aisb_0 & Co. to get these pieces together 👨🏻‍⚕️👩🏽‍⚕️🔬!

@aisb_0

Ali Isb

4 years

Aaand here's our protocol on microscopy-based analysis of GPCR oligomerization and concentration in 2 ways. It comes with a bonus protocol describing 3 strategies to clone receptors made to shine under lasers! Just out on @NatureProtocols !

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@HSchihada

Hannes Schihada, PhD

4 years

What an episode!

@DrGPCR

DrGPCR

4 years

The wait is over! The first episode of 2021 of the Dr. GPCR Podcast is now available. My guest is Dr. Robert J. Lefkowitz. #gpcr #drgpcr

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@HSchihada

Hannes Schihada, PhD

4 years

Thumbs up for this #integrity !👍👍

@nicolajsmith78

Nicola J Smith

@nicolajsmith78

4 years

@zenbrainest The most horrific time of my career, discovering a cloning error from 2012 that was propagated through the years. We lost the first two papers of my independent career and valued collaborators were collateral damage through no fault of their own. @smith_orphans 1/n

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@HSchihada

Hannes Schihada, PhD

3 years

@aisb_0 @SteffenPockes That was a fantastic collaborative effort with precious #microscopy 🔬data from you @aisb_0 & Jan Möller! No reason to play down your own impact, mate ;-)

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@HSchihada

Hannes Schihada, PhD

4 years

@aisb_0 "smFRET-RAP" 😆 What comes next? smFRET-dcRAP-based SAR?

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@HSchihada

Hannes Schihada, PhD

2 years

Registration is NOW open! And don't forget to check out our Action webpage #CA18240 to stay tuned and don't miss any news regarding this and other #aGPCR -related events!

Adher'n Rise Training School 2023: Cell-based assays to study aGPCR function

time: June 28th - June 30th 2023 (full days) venue: Karolinska Institutet, Biomedicum, Solnavägen 9, 17165 Solna, Sweden If there are any questions, please contact us at: [email protected] and...

docs.google.com

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@HSchihada

Hannes Schihada, PhD

3 years

@HanaScientist You might find these sensors useful, developed by the lab of Michel Bouvier:

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Development of conformational BRET biosensors that monitor ezrin, radixin and moesin activation in...

Summary: Characterization of BRET-based conformational biosensors that monitor individual ezrin, radixin or moesin activation at the plasma membrane in living cells.

journals.biologists.com

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@HSchihada

Hannes Schihada, PhD

4 years

@ClaudioCantu81 I received exactly the same response upon a plasmid request! I never answered cause I don’t feel that this how science should work. I was also considering to contact the journal and tell them about this issue but never did...

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@HSchihada

Hannes Schihada, PhD

3 years

Check out our preprint about PTH1 receptor function by RAMP2! @karantarina did a great job in front of the 🔬to discover that interaction with RAMP2 allows the #GPCR to switch faster into the active state!

@karantarina

Katarina Nemec

3 years

I am beyond happy that I can share our manuscript with the community! #GPCR + #RAMP interplay from a collaborative effort of Lohse lab/Receptor signaling lab @MDC_Berlin with @HSchihada , Scheerer lab @ChariteBerlin , Martin J.Lohse, and Isabella Maiellaro.

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@HSchihada

Hannes Schihada, PhD

3 years

Very interesting preprint revealing a new player in #GPCR - #arrestin interaction!

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@HSchihada

Hannes Schihada, PhD

4 years

And another one today! But it’s too early for the weekend. Therefore only a small one 😉

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@HSchihada

Hannes Schihada, PhD

4 years

@aisb_0 I think that should be on the designer's table but what about "NIGHT"-tag for "Non-InternalizinG HaloTag Tandem" ;-)

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@HSchihada

Hannes Schihada, PhD

4 years

#gamechanger 😍

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@HSchihada

Hannes Schihada, PhD

1 year

@DetlefObal @Carvvver @THebertMcGill @stanfordanes @StanfordCVI It was fun reading your work! Congrats on the publication!

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@HSchihada

Hannes Schihada, PhD

3 years

What an inspiring story ⁦ @rhodamine110 ⁩! Open Chemistry: What if we just give everything away? | eLife

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@HSchihada

Hannes Schihada, PhD

2 years

Gab‘s schon die Idee, das Arbeitnehmerrecht auf (mehr) Home Office wie zu Corona-Zeiten politisch zu unterstützen, um Berufspendler an der Zapfsäule zu entlasten und durch gesunkene Nachfrage den Spritpreis über den Markt zu senken? @hubertus_heil @c_lindner @Wissing

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@HSchihada

Hannes Schihada, PhD

4 years

@AliceInNanoland @IvanMasl0v @manorlaboratory Maybe check the publications from JP Pin's and P Rondard's groups including this one: . They used SNAP and Halo to monitor conformational changes and dimer movements of GPCRs

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@HSchihada

Hannes Schihada, PhD

11 months

@MiriamStoeber @unige_en Congratulations Miriam! Well deserved!

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@HSchihada

Hannes Schihada, PhD

1 year

@Carvvver @stanfordanes @StanfordCVI @DetlefObal @THebertMcGill Very thorough work, congrats!

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@HSchihada

Hannes Schihada, PhD

1 year

@schulte_lab @GraetzLukas Congrats to everyone involved!

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@HSchihada

Hannes Schihada, PhD

3 years

@ScienceTwitter : Are there scientific #journals that particularly welcome submissions of manuscripts that report on conflicting results? #AskingForAFriend

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@HSchihada

Hannes Schihada, PhD

3 years

Don't miss this talk from @DrGPCR and join us!

@ca18240

CA18240 Adher´n Rise

3 years

@DrGPCR is presenting her path to building this fantastic #GPCR ecosystem at our virtual conference in only one hour! So excited to hear you talk about marrying GPCRs with #science #communication & #entrepeneurship ! #AdhernRise #ca18240

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@HSchihada

Hannes Schihada, PhD

3 years

@LiebscherLab @Amro_Hussien @wilde_caro2 @gstephan1507 @placantonakilab @_Sandra_Berndt_ you can get them all in one case 😉

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@HSchihada

Hannes Schihada, PhD

3 years

@AliceInNanoland Both are permeating well but we’ve seen much more unspecific labeling with intracellular SNAP dyes in human cell lines. SNAP-tag was engineered from a mammalian enzyme that is also expressed in our cell line while HaloTag from a procariotic protein. So it makes sense somehow…

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@HSchihada

Hannes Schihada, PhD

2 years

@PKozielewicz Congrats @PKozielewicz @GraetzLukas @schulte_lab !

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@HSchihada

Hannes Schihada, PhD

3 years

@LoogerL @UCSD_NIC @joachimgoedhart @LABrechaun @NathanShaner Very true! I didn’t wanna say that Halotag doesn’t mess up with protein conformation. But it allows for easy acceptor exchange without additional cloning and switching from one label to another, which would likely change the overall conformation of the engineered fusion construct

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@HSchihada

Hannes Schihada, PhD

3 years

@DrGPCR @Michel_Bouvier @IRIC_umontreal Such an inspiring interview! What a pity @Michel_Bouvier had a hard stop at 10 😅

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@HSchihada

Hannes Schihada, PhD

2 years

@ReidHJOlsen @jbiolchem @schulte_lab @shamimdadvar @ruaslab @Laura_Humphrys Thanks Reid 😊

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@HSchihada

Hannes Schihada, PhD

4 years

@_Toby_Thomas_ @mikefeigin @DrGPCR I can highly recommend the FRET-based cAMP Sensor “H187” from the Jalink lab. Works well in a single-cell and 96-well plate format.

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@HSchihada

Hannes Schihada, PhD

3 years

@wilde_caro2 @ca18240 Such a smart approach! I was looking forward to read this preprint since I heard about the method in the @DrGPCR podcast with @LiebscherLab & @ProemelLab ! Congrats to all authors and 🤞 for the peer review!

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@HSchihada

Hannes Schihada, PhD

1 year

@ThePhDPlace „We only need this one more control and then we can wrap up the story.“

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@HSchihada

Hannes Schihada, PhD

4 years

@aisb_0 @PNASNews @ErnestNetwork Such a cool story! Huuuuuuge Congrats to you and all the other authors!

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@HSchihada

Hannes Schihada, PhD

4 years

Such a cool finding!

@Michel_Bouvier

Michel Bouvier

@Michel_Bouvier

4 years

From a discussion at a Gordon Conference, where Nevin Lambert and I realized that we had similar observations about an unexpected behaviour of G12, came this nice collaboration paper.

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@HSchihada

Hannes Schihada, PhD

3 years

This is a great chance for members of ITCs to join the next @ErnestNetwork meeting in Bari 🇮🇹 and the associated workshop on signal transduction mapping! #adhesion #GPCR #conference #AdhernRise

@ca18240

CA18240 Adher´n Rise

3 years

📢Our action #ca18240 offers members of Inclusiveness Target Countries to apply for travel grants to join the next @ErnestNetwork meeting in October in Bari 🇮🇹 Application deadline: 15.08.2021 Check our homepage for more info: #GPCR #aGPCR #AdhernRise

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@HSchihada

Hannes Schihada, PhD

3 years

@karantarina Congrats Katarina!

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@HSchihada

Hannes Schihada, PhD

3 years

@ReidHJOlsen Related to that, I had many conversations with people claiming one assay being the best cause the delta BRET was highest. Well..different read parameters, transfection ratios and analyses often render these comparisons impossible. Plus, amplitudes are not the only factor… ➡️ #Z ‘

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@HSchihada

Hannes Schihada, PhD

4 years

@AliceInNanoland @IvanMasl0v @manorlaboratory Hey Jia! HaloTag works very well for that but we didn't have that good results with SNAP-tag. However, we were limited to use cell-permeable dyes only and that might have been the problem there. I know that SNAP-tags works very well for extracellular labeling.

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@HSchihada

Hannes Schihada, PhD

1 year

@LiebscherLab @MiriamStoeber And of course: A big thank you to @COSTprogramme for making this event possible & to Lukas Grätz @GraetzLukas for organizing this with me!

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