Katie Galloway Profile Banner
Katie Galloway Profile
Katie Galloway

@GallowayLabMIT

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Assistant Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo

Joined June 2019
Don't wanna be here? Send us removal request.
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@GallowayLabMIT
Katie Galloway
2 years
Especially delighted we could cast a vision of DNA supercoiling on the cover. Image by Christopher Johnstone.
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@GallowayLabMIT
Katie Galloway
2 years
How does transcription of proximal genes influence the expression of their neighbors? How do we engineer compact circuit designs for specific profiles of expression that enable robust cellular engineering? Today @CellReports we provide some answers (1 /n)
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@GallowayLabMIT
Katie Galloway
2 years
It's only lineage if you come from the Cambridge region of Boston, otherwise it's just sparkling academic nepotism.
@pdhsu
Patrick Hsu
2 years
What are the most impressive academic lineages in the biological sciences?
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@GallowayLabMIT
Katie Galloway
3 years
pro-tip: you can just delete every instance of "has been shown to" to switch to active voice This writing tip has been shown to work. This writing tip works.
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@GallowayLabMIT
Katie Galloway
1 month
in case you're wondering how stable the 3D genome is
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@GallowayLabMIT
Katie Galloway
2 years
How does transcription of proximal genes influence the expression of their neighbors? How do we engineer compact circuit designs for specific profiles of expression that enable robust cellular engineering? Today @CellReports we provide some answers (1 /n)
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@GallowayLabMIT
Katie Galloway
15 days
Levels of gene expression matter! So how can you control a transgene precisely? Is there a tool that can ensure uniform control & the ability to adjust the setpoint of expression from a single promoter? Introducing DIAL! 🧵 (1/n) Link:
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@GallowayLabMIT
Katie Galloway
3 years
First NOA!!! Thrilled to announce that the Galloway Lab received a 5 year $1.94 M @NIGMS R35 MIRA ESI grant! This will fund our work to understand the molecular processes that support cellular reprogramming. SO PROUD of our lab for their hard work! @MITChemE
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@GallowayLabMIT
Katie Galloway
9 months
How do individual transcription factors (TF) influence reprogramming? Isn’t it just the cells with the highest levels of TF expression that reprogram? So just blast them & it should work, right? 🤨Buckle up for our lab’s preprint & tour de force led by Nat Wang @nbwang22 ! (1/n)
@biorxiv_cellbio
bioRxiv Cell Biology
9 months
Proliferation history and transcription factor levels drive direct conversion #biorxiv_cellbio
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@GallowayLabMIT
Katie Galloway
4 years
In wake of all this chaos, I got some excellent news! My husband is officially cancer-free at his 6 month scan! So relieved and grateful to be able to share this news! Thankful for all who helped including @MITChemE who helped us find an oncologist in Boston #thanksMGH #PraiseGod
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@GallowayLabMIT
Katie Galloway
2 years
Every year in September people start asking for advice about going on the job market. I cringe bc for most people September is too late. You need to start planning NOW! So how can you start preparing now? A short 🧵(1/6)
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@GallowayLabMIT
Katie Galloway
7 months
Excited to say our lab's about to do some "understanding and engineering" with the support of the NSF CAREER Award! Really grateful for fantastic work by my students and support from my department @MITChemE Delighted for the research and outreach this will support. Excelsior!
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@GallowayLabMIT
Katie Galloway
2 years
Happy "end of early career" to all that celebrate😅 if your awards count time from PhD, you should fix that... with love, from all the moms in science
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@GallowayLabMIT
Katie Galloway
4 years
Every new PI trying to launch a lab this year...
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@GallowayLabMIT
Katie Galloway
3 years
Our hearts are breaking at @MITChemE . On Friday, we lost Jim Swan @Jim_Swan . Jim was a wonderful mentor, colleague, and friend. We had so much left to learn from you, Jim... 😭
@Jim_Swan
James Swan
3 years
The opportunity to know, work with, and mentor such remarkable young people is undoubtedly the best perk of this job.
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@GallowayLabMIT
Katie Galloway
3 years
Can we please dispense with early career definitions by degree date? Or are we just content tacitly saying "you'll be sorry if you take time off (to take care of your family)" ? #earlycareer @momadamia #momadamia @AcademicChatter
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@GallowayLabMIT
Katie Galloway
4 years
1/n Rounding out the best of 2020 for our lab, newly published “Engineering Cell Fate: Applying synthetic biology to cellular reprogramming.” We review cellular reprogramming+ #synbio Vision brought to life in vivid color by @nbwang22 and @beitz_adam
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@GallowayLabMIT
Katie Galloway
3 years
this is called PI-hacking
@EikoFried
Eiko Fried
3 years
My students invented Eiko bootstrapping: you ask the PI the same question a few times, 4-8 weeks apart, and he'll have forgotten he was asked the question before. If he gives the same answer multiple times, we'll call it a robust and reliable outcome.
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@GallowayLabMIT
Katie Galloway
3 years
What is it like to run a startup lab? #3ringcircus
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@GallowayLabMIT
Katie Galloway
2 years
Excited to share our new @biorxivpreprint ! Integrating DNA biophysics with classic #synbio to map how to improve transgenic systems by tailoring expression levels, variance, and transcriptional dynamics. TL;DR: Don’t build your systems to fail. Build beautiful dynamics! 🧵(1/n)
@bioRxiv_synbio
bioRxiv Synthetic Biology
2 years
Supercoiling-mediated feedback rapidly couples and tunes transcription #biorxiv_synbio
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@GallowayLabMIT
Katie Galloway
4 years
How it started How it's going
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@GallowayLabMIT
Katie Galloway
5 years
Decade reflections: A decade ago I was a struggling grad student at Caltech with a 5-month old daughter. My entire lab had moved the prior year to Stanford. My husband was at UCLA. My journal says, "things just keep not working" and that was definitely a journal theme...
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@GallowayLabMIT
Katie Galloway
4 years
The lab's first paper is accepted!!! yasss! Congrats @nbwang22 and @beitz_adam ! Excited to share our review on the synthetic biology of cellular reprogramming when it's published!
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@GallowayLabMIT
Katie Galloway
3 years
peer review would be 25% faster if we didn't have to reset our password every time we logged into editorial manager... #justretinascanme
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@GallowayLabMIT
Katie Galloway
10 months
If you missed me at AIChE, I'm sorry. I didn't realize I wearing Florida camouflage. I am this plant.
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@GallowayLabMIT
Katie Galloway
2 months
on unimodal setpoints and how to get them from synthetic promoters... tweetorial coming soon... or see you next Thursday #SEED2024 in Atlanta... Stay tuned for more control systems coming soon...
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@bioRxiv_synbio
bioRxiv Synthetic Biology
2 months
Programmable promoter editing for precise control of transgene expression #biorxiv_synbio
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@GallowayLabMIT
Katie Galloway
3 years
Come be our colleague! MIT ChemE is hiring!!! @MITChemE @ChEnected
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@GallowayLabMIT
Katie Galloway
8 days
Transgenics have the potential to massively expand the power of cell therapies. However, transgenesis adds a step in the already substantial challenge of scaling up cell-based therapies. How do we ensure that cell therapies can harness the power of synthetic biology? 🧵 (1/n)
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@GallowayLabMIT
Katie Galloway
3 years
Its signing day @MITChemE ! Delighted to be welcoming Emma Peterman @emmapeterman_ to lab. She joins us from UD as an alumna of @cfromen lab. Excited to watch Emma launch into building synthetic models of tissue regeneration! (Also, yes, signing day is now signing month)
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@GallowayLabMIT
Katie Galloway
2 years
This is a very clever approach to control expression. They engineer recruitment of transcriptional activators fused to RNPs to sites of nascent transcription by embedding RNP-binding sequences within the intron @NatureComms
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@GallowayLabMIT
Katie Galloway
2 years
Hey ChemEs, We're hiring! Come be our colleague!
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@GallowayLabMIT
Katie Galloway
10 months
Do you know who Grace Hopper is? You should. "One of the first programmers of the Harvard Mark I computer, she was a pioneer of computer programming who invented the first compiler." She is an inspiration! Happy Halloween!
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@GallowayLabMIT
Katie Galloway
3 years
I always appreciate when authors utilize an axis of scale...this one has TWO for understanding chromatin organization, imagining techniques, and mechanisms that support structure and dynamics!
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@MolSystBiol
Mol Syst Biol
3 years
Review on the applications of #singlecell #imaging approaches for analysing genome folding and dynamics --> from Liangqi Xie & James Zhe Liu @HHMIJanelia #genomearchitecture #3Dgenome #chromatin
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@GallowayLabMIT
Katie Galloway
3 years
grief isn't linear or homogenous; it's an asynchronous, heterogeneous process; as with all time-varying phenomena, a single snapshot may not capture the picture, so be kind and generous to those around you
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@GallowayLabMIT
Katie Galloway
3 years
tomorrow at #AICHE2021 i will say some things
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@GallowayLabMIT
Katie Galloway
1 year
@dgonzales1990 3 things have been useful. 1. Journal Club. I recommend leading the first few on a single paper. Deep dive. 2. Speed JC. 1 topic; 5-8 papers. 1 slide per paper. Helps ppl see themes. Everyone gets 2-3. 3. Lit commentary channel on slack to share as papers are posted...(1/n)
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@GallowayLabMIT
Katie Galloway
2 years
Yes, where you train is important but let's not become academic aristocrats. That's just the silliest path to bad outcomes.
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@GallowayLabMIT
Katie Galloway
3 years
As Yoda says...
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@GallowayLabMIT
Katie Galloway
5 years
And the new website is live!!!
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@GallowayLabMIT
Katie Galloway
3 years
Today Massachusetts will cross the 50% vaccination rate...so grateful and relieved for the work of so many to end the pandemic...(joy)
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@GallowayLabMIT
Katie Galloway
2 years
Do you have a favorite guide or tutorial for writing papers? How do you approach thinking through structure? Engineering vs biology seem to require different approaches to structure. Given his papers, I bet @MoKhalilLab has this down to precise art. Any tips, docs, resources, Mo?
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@GallowayLabMIT
Katie Galloway
4 years
each year I give a state of the lab (SOL) to introduce the lab to new students and summarize where we are and where we're going...I wanted a way to capture the founding members of the lab... @nbwang22 sent me this...🤣🤣🤣 love it!
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@GallowayLabMIT
Katie Galloway
4 years
15 years ago! Best decision ever! Fortunate to have an unbelievably supportive partner...especially in these crazy times, this has made all the difference in facing the many challenges and joys
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@GallowayLabMIT
Katie Galloway
3 years
What limits synthetic control over cellular behaviors? How can gene circuits be used to buffer or harness transcriptional noise in achieve predictable multicellular responses? Where does #sybio go from here? Read on! @ChrisPJohnstone @MITChemE @NatRevMCB
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@GallowayLabMIT
Katie Galloway
4 years
What's your definition of synbio? I'll start. Synbio is the systematic engineering of native biological systems to extract design principles (e.g. build-to-understand) and construct novel tools, devices, and systems. Ok, now your turn! ( I expect a wide variance in opinion ;))
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@GallowayLabMIT
Katie Galloway
3 years
90% of being a PI is just updating your biosketch... and obviously the other 10% is tweeting about it🙃
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@GallowayLabMIT
Katie Galloway
4 years
A year ago I got in the car with my family to drive to from California to Massachusetts and start a lab. Today our lab submitted two manuscripts (reviews). It's been a wild year. But the memes from the students are the best. Below is how we make decisions on figures.
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@GallowayLabMIT
Katie Galloway
3 years
Perhaps the greatest challenge in growing up is to see the world as it really is and yet not grow cynical or weary; to retain courage and hope, no matter what looms on the horizon.
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@GallowayLabMIT
Katie Galloway
4 years
Just got the news, at MIT, we're going to 100% capacity tomorrow!!!!!!!!!! *tears of joy*
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@GallowayLabMIT
Katie Galloway
2 years
"The light shines in the darkness and the darkness has not overcome it." John 1:5 May Light and Life guide you and fill you in the coming year! Merry Christmas!
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@GallowayLabMIT
Katie Galloway
6 months
Got to write a bit for @NatureRevGenet on what sparked my love of the cell cycle as a (underappreciated) determinant of cell fate. See here: For the lovely work from @Kueh_Lab when he worked with @ElowitzLab see here:
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@GallowayLabMIT
Katie Galloway
4 years
Rising Stars @MITChemE Enjoying getting to meet so many fabulous future faculty! Fun to see @Asher__Williams @DrRituRaman @cemills3 @sikeslab @afurst1 and many others!
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@GallowayLabMIT
Katie Galloway
10 months
Western, Northern, and Southern blots from the Galloway lab!
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@GallowayLabMIT
Katie Galloway
5 years
It's Signing Day @MITChemE ! Excited to sign with these three awesome (and brave) founding grad students... In honor of signing day, we got tshirts like grad lab selection is some kinda NCAA sport 😄...intros to follow...
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@GallowayLabMIT
Katie Galloway
4 years
@MoatazAttallah The antibody showed some non-specific binding.
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@GallowayLabMIT
Katie Galloway
3 years
it begins...
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@GallowayLabMIT
Katie Galloway
3 years
This is not my experience at MIT. My latest recurring meeting ends at 5:30 pm. I don't have any recurring weekend meetings. My department @MITChemE supports its parents thru time & finances. Excellent research, brilliant students, massive support. To me, "This is MIT."
@grok_
Kate Darling
3 years
Multiple times senior professors have asked to schedule recurring meetings in the evenings or on weekends and when I gently told them that those times are difficult for parents, they responded along the lines of “well this is MIT”. And yet our diversity issues persist! Curious.
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@GallowayLabMIT
Katie Galloway
3 years
Finally getting to meet your twitter and #newPISlack friends in real life is a highlight of post-vax life. Fun times @baym ! Cheers to more great time chatting about science and startup!
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@GallowayLabMIT
Katie Galloway
1 year
An unnamed hero, the folks at @JetBlue , and my awesome postdoc @KeiTakahashi720 got it back to me!!! I'm happy to be in CA but happy to know I can get on the plane home 🤣
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@GallowayLabMIT
Katie Galloway
1 year
If there's a hero at Boston Logan that finds a blue purse near gate C10, I'm happy to offer a reward.
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@GallowayLabMIT
Katie Galloway
3 years
How it started How it's going (12 years later)
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@GallowayLabMIT
Katie Galloway
3 years
The mechanical genome😍"...negative supercoiling generated by transcription is an important regulator of loop formation in vivo."
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@MolecularCell
Molecular Cell
3 years
Online Now: Transcription-mediated supercoiling regulates genome folding and loop formation
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@GallowayLabMIT
Katie Galloway
2 years
If Twitter dies, then thanks to you all who shared beautiful science, sassy reflections, and provided a window into your world. It has been a joy to find connection over these last 3 years. Resurgam et Excelsior!
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@GallowayLabMIT
Katie Galloway
3 years
yesterday was my "academic birthday." I defended my thesis 9 years ago ⏳ 😮 Like most "birth" days it was the beautiful culmination of lots of hard work, hope, passion, and pure grit...but it wasn't pretty... it was rough...
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@GallowayLabMIT
Katie Galloway
1 month
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@markwbudde
Mark Budde
1 month
Trust nothing. Validate everything.
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@GallowayLabMIT
Katie Galloway
6 months
how I started to see email when i started a lab
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@GallowayLabMIT
Katie Galloway
2 years
Met this handsome guy in San Antonio getting off a plane from Boston. It was love at first sight 😍 Had a great Cinnbon date #MrGalloway
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@GallowayLabMIT
Katie Galloway
3 years
Oh Boston, you are beautiful 😍
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@GallowayLabMIT
Katie Galloway
1 year
Fun times running around London on a beautiful day! Excited to be heading to Cambridge for #SynBioFH23 . Thanks for the tips @ProfTomEllis !
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@GallowayLabMIT
Katie Galloway
2 years
Hello Edinburgh! Lovely, historic city! 😍
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@GallowayLabMIT
Katie Galloway
5 years
MIT has this lovely month-long "independent activity period" (IAP) and it's amazing! No required classes or admin, just research and short courses in January. It was a great time training new team members in lab. Now back to the real world!
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@GallowayLabMIT
Katie Galloway
3 years
I got such a sweet gift from my lab! 2021 has been filled with such wonderful memories, rigors and challenges, and some very hard things. Knowing they are with me has been the energy and the encouragement that I have needed. In 2022, excelsior!
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@GallowayLabMIT
Katie Galloway
3 years
🎉Congratulations to our very own Ph.D. student Adam Beitz @beitz_adam for being named a 2021 NSF Graduate Research Fellow! 🎉 @NSFGRFP @MITChemE @MITEngineering
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@GallowayLabMIT
Katie Galloway
3 years
First day of teaching my new elective in-person...over 18 months, I had forgetten the adrenaline rush (and intense post-lecture exhaustion) of standing in front of a live room; I look awake but I am tired
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@GallowayLabMIT
Katie Galloway
4 years
I have a joke about control systems, but it's received a lot of negative feedback.
@michaelhoffman
Michael Hoffman @michaelhoffman.bsky.social
4 years
I have an epigenetics joke, but I'm not going to clarify whether I mean persistent cellular memory or simply transcriptional regulatory state.
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@GallowayLabMIT
Katie Galloway
2 years
Congrats to Brittany Lende and Kasey Love @kaseyslove on receiving the @NSF Graduate Research Fellowship #NSFGRFP @MITChemE @MITdeptofBE
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@GallowayLabMIT
Katie Galloway
4 years
1/n So you love the 3D genome and want to know more about how synthetic biology will address open question in the structure and function of chromatin? This review is for you! (And it’s open access!) Think @4DNucleome meets #synbio -> @CellSystemsCP
@nbwang22
Nat B. Wang 🧬🧑🏻‍🔬🏳️‍🌈
4 years
After several months and lots of work, our review is finally out! In it we discuss how 🧬sequencing, 🔬 microscopy, and 🧑‍🔬 synbio approaches can be used to both understand and engineer chromatin as a dynamic system! @GallowayLabMIT @ChrisPJohnstone
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Katie Galloway
2 years
Before CRISPR, what did ppl used for engineering mammalian cell lines and animals? Transposon systems, etc? I'm reading about "stable" transfection. And what is the mechanism!?! Is this just chucking genetic cargoes into the cell and hoping things work out? I bet @chorye knows
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@GallowayLabMIT
Katie Galloway
1 year
Gorgeous evening in Boston!
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@GallowayLabMIT
Katie Galloway
2 years
First Galloway Lab Beach Day was a blast! Fun times in honor of our most recent PhD candidates, Sneha Kabaria @science_sneha and Brittany Lende! Welcome summer! @MITChemE
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@GallowayLabMIT
Katie Galloway
10 months
Looking forward to visiting Cornell! See ya soon!
@CornellBME
Cornell BME
10 months
Incoming this Friday! We welcome our next @CornellEng @CornellBME #Seminar7900 speaker, Dr. Katie Galloway @GallowayLabMIT of @MITChemE , speaking on "Engineering High-precision, Dynamic Genetic Control Systems for Cellular Reprogramming."
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Katie Galloway
4 years
“Work hard and don’t compromise who you are.” #DrMom #WomenInSTEM
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@Megan_L_Norris
Dr. Megan L Norris
4 years
“Work hard and don’t compromise who you are.” #DrMom #WomenInSTEM
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@GallowayLabMIT
Katie Galloway
1 year
My 11 yo is playing Oregon trail with our family as characters. 11 yo: Mom, you have died of exhaustion. You were the first to go. Me:
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@GallowayLabMIT
Katie Galloway
4 years
Delighted to officially welcome Kasey Love ( @kaseyslove ) to the group! Kasey is a first-year BE PhD student hailing from Stanford, who enjoys hiking, watching soccer, and building genetic controllers that regulate stem cell fate. Welcome Kasey! @MITdeptofBE @MITChemE
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Katie Galloway
4 years
GRE no more at @MITChemE Now send us your apps! ;)
@cwcoley
Connor W. Coley
4 years
After a decisive faculty vote, @MITChemE has decided to drop the GRE (not just for this year) for graduate admissions. Thanks to many current PhD students for helping make the push for #GRExit
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Katie Galloway
4 months
Hey there @Caltech folks! Want to hear about cell fate programming and genetic controllers? I'll see you tomorrow 4 pm in 106 Spalding
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@GallowayLabMIT
Katie Galloway
4 years
Kiddo: can I brush you hair? Me: yes. (Typing furiously while my stylist works) Kiddo: can I help you put on deodorant? Me: yes. (More typing, more help) Kiddo: can I do your makeup? Me: sure. (More typing, more help) Hurray I edited a paragraph! And got a makeover!
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@GallowayLabMIT
Katie Galloway
4 years
failures of three, let it be...
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@GallowayLabMIT
Katie Galloway
4 years
I'm excited to announce we've officially survived 4 weeks with the kids at home and have toilet paper and paper towels! Celebrating the minor victories!
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@GallowayLabMIT
Katie Galloway
2 years
@TanentzapfLab True, but sometimes not knowing "the right answer" helps ppl make progress in ways that those who know "the right answer" cannot.
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@GallowayLabMIT
Katie Galloway
3 years
The 4 year old this morning: You know what the best gift is, Mom? Me: What? Him: Love. Merry Christmas everyone! The greatest gift is love! 1/n
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@GallowayLabMIT
Katie Galloway
3 years
if you're on the market, you're going to get asked these questions (and some you will get over and over again). Take the time to think on your answers now and be ready! You got this!
@GarciaLabMS
Ben Garcia
3 years
Our junior faculty search committee has decided on the top 6 candidates to invite for in person interviews. Here are the questions we asked in the Zoom interviews. These are similar to what most places ask from what I gather, but wanted to post these to help anyone about to 1/
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@GallowayLabMIT
Katie Galloway
4 years
Delighted to be welcoming Binette Wada @binettem11 as our group's newest UROP. Binette is a sophomore in chemical engineering @MITChemE . This summer she's deploying her awesome modeling skills in the development of genetic controllers for reprogramming. Welcome Binette!
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@GallowayLabMIT
Katie Galloway
5 months
Come join us @MITChemE for Rising Stars! Women interested in preparing for the academic job market, this event is for you!
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@GallowayLabMIT
Katie Galloway
2 years
To the nameless hero that quickly intervened when a belligerent woman aggressively came after me as I walked to my ACS morning session, thank you! I'm stunned but fine. Very grateful for this kind, brave stranger.
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@GallowayLabMIT
Katie Galloway
3 years
Achievement unlocked! Getting to celebrate 4th of July with my lab...a dream 2 years in the making! Even in the rain the fireworks were worth it! Such a great view from @MITChemE
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@GallowayLabMIT
Katie Galloway
1 month
😮😅🤣"after dehydration loops remain (top center), even after a year at room temperature (top right), and even if (bottom right) the sample is subsequently blasted with a shotgun, run over by a car, or hit with a fastball."
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@R_Stadhouders
Ralph Stadhouders
1 month
@GallowayLabMIT Just wait untill you read Fig.6D 😎
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@GallowayLabMIT
Katie Galloway
4 years
The lab's second paper is accepted!!! Congrats @ChrisPJohnstone , @nbwang22 , and Stuart Sevier This was a long-planned review and big lift! I will be very excited to share our perspective on the emerging synthesis between worlds of #SynBio and the @4DNucleome when posted!
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@GallowayLabMIT
Katie Galloway
3 years
Person 1: The glass is half empty Person 2: The glass is half full Synthetic biologists: Have we tried tagging the glass with GFP?
@michaelhoffman
Michael Hoffman @michaelhoffman.bsky.social
3 years
Person 1: The glass is half empty Person 2: The glass is half full Genomicist: Let's sequence the contents of the glass!
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@GallowayLabMIT
Katie Galloway
3 years
Traveling with kids is always a trip but after almost 2 years we're thrilled to finally be back visiting SoCal, family, and friends
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@GallowayLabMIT
Katie Galloway
3 years
My out of office: If you're reading this, I'm in California...email will be slow. If you're not reading this, our email-to-telepathy experiment has finally worked.
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@GallowayLabMIT
Katie Galloway
3 years
That's my rockstar, trailblazing dept chair! 😍 #blackinstem #BlackinTech @BlkInEngineerng @MITChemE
@cenmag
C&EN (Chemical & Engineering News)
4 years
“This issue on C&EN’s Black Trailblazers has special meaning to me. I know the impact of having scientific role models who have the same heritage and background, who have faced the same obstacles,” says Paula Hammond, guest editor for this upcoming issue publishing on Feb. 22.
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