Kai Chen @kaichen_1990 Twitter profile

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Kai Chen

@kaichen_1990

5 years

Our review of “engineering new catalytic activities in #enzymes ” is online at @NatureCatalysis , highlighting the work from @HysterLab , @JCLewisLab , @WardGroupBS , @Turner_Biocat , @RoelfesGroup , @FasanLab , Müller, Hauer, us @francesarnold lab and more.

Engineering new catalytic activities in enzymes

Nature Catalysis - Advances in enzyme performance and capabilities are making them increasingly attractive to synthetic chemists. In this Review Chen and Arnold outline the ways that enzymes have...

www.nature.com

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Kai Chen

@kaichen_1990

4 years

Congratulations! Finally Crispr!!!!!! Never imagined having the chance to witness both PhD advisor @francesarnold and postdoc advisor, Jennifer Doudna, awarded the Nobel Prize in chemistry before 2018!

The Nobel Prize

@NobelPrize

4 years

BREAKING NEWS: The 2020 #NobelPrize in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”

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Kai Chen

@kaichen_1990

3 years

I am saddened to know my PhD committee professor, Bob Grubbs, one of the greatest chemists, 2005 chem Nobel laureate, passed away this morning. He was so humble (when I first saw him sitting on the ground listening to a talk) but serious in science and helpful in my PhD. RIP.

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Kai Chen

@kaichen_1990

4 years

No longer a candidate... Finally became a real PhD. Great thanks to my committee, @francesarnold @Harry_and_Jay @FuLabCaltech and Bob Grubbs! And also to everyone who joined my defense today!

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Kai Chen

@kaichen_1990

11 months

Today I want to share two stories of my postdoc research @doudna_lab . The first project in collaboration with the Murthy lab at Berkeley describes our work on “lung and liver editing using lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP”.

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP

Lipid nanoparticle (LNP) delivery of CRISPR ribonucleoproteins (RNPs) has the potential to enable high-efficiency in vivo genome editing with low toxicity and an easily manufactured technology, if...

www.biorxiv.org

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Kai Chen

@kaichen_1990

3 years

Over a year after my graduation, our newest story is finally out @NatureChemistry ! Check it out if you want to learn how enzymes can be engineered to precisely position intermediates and drive exquisite selectivity for a non-natural activity.

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Kai Chen

@kaichen_1990

8 months

The first paper of this year, “Engineering self-deliverable ribonucleoproteins for genome editing in the brain”, is officially out @NatureComms . This is the first project of my postdoc @doudna_lab , where we focused on delivery method development for CRISPR genome editors in the

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Kai Chen

@kaichen_1990

5 months

Our paper on the biochemical understanding of an engineered Cas9 (by directed evolution) is officially out @CellCellPress . “Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9” Following our previous study on the development of an

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Kai Chen

@kaichen_1990

4 years

Found this wonderful picture of the two Nobel laureate advisors!

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Kai Chen

@kaichen_1990

11 months

My second project @doudna_lab also has a topic on RNP delivery for genome editing in vivo. Different from using LNPs to package and transport gene editors, we engineered self-deliverable Cas9 RNPs through fusion to cell-penetrating peptides (CPPs).

Engineering self-deliverable ribonucleoproteins for genome editing in the brain

The delivery of CRISPR ribonucleoproteins (RNPs) for genome editing in vitro and in vivo has important advantages over other delivery methods, including reduced off-target and immunogenic effects[1...

www.biorxiv.org

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Kai Chen

@kaichen_1990

4 years

I am so thrilled and touched to watch this announcement by my advisor @francesarnold ! It has been my honor to have such a great advisor, an incredible committee and everyone around me, who have been giving me trememdous support throughout my whole PhD @Caltech ! So grateful!

Resnick Sustainability Institute at Caltech

@caltechRSI

4 years

Wow! Congrats @kaichen_1990 😎👏👏🔥👏 #caltech2020

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Kai Chen

@kaichen_1990

5 years

2y ago, we engineered P450 enzymes to build strained rings, cyclopropenes and bicyclobutanes, with terminal alkynes. Now more powerful P450s used internal alkynes to make cyclopropenes. Check out our paper from @francesarnold lab and guess what is next...

Engineering Cytochrome P450s for Enantioselective Cyclopropenation of Internal Alkynes

We report a biocatalytic platform of engineered cytochrome P450 enzymes to carry out efficient cyclopropene synthesis via carbene transfer to internal alkynes. Directed evolution of a serine-ligated...

pubs.acs.org

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Kai Chen

@kaichen_1990

10 months

Congratulations to everyone on the paper! I guess this is the last paper from my PhD (as a co-corresponding author) with @francesarnold This story showcases how enzymes engineered by #directedevolution can acquire new abilities to build a broad set of molecular structures with

Runze Mao

@runzemao

10 months

New Year, New Paper🚀Today in @J_A_C_S , we report a biocatalytic platform that employs a C–C bond disconnection strategy to broaden Nature's lactone synthesis approaches, which rely solely on a C–O bond disconnection.

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Kai Chen

@kaichen_1990

6 years

See, who is new on the Nobel wall! @francesarnold

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Kai Chen

@kaichen_1990

2 years

The first little story of my postdoc research @doudna_lab on engineering Cas12a gene editors for improved efficiency. Thanks to my collaborator Enbo and everyone else involved. Hopefully, more #directedevolution stories will follow.

Doudna Lab

@doudna_lab

2 years

New paper on engineered Cas12a variants! Through both structure guided engineering and directed evolution we minimized trans activity, increased genome editing in human cells, and enhanced HDR efficiencies @igisci

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Kai Chen

@kaichen_1990

7 months

So excited to see the highlight by @natrevbioeng on our recent work of brain editing using self-deliverable CRISPR genome editors @doudna_lab .

Nature Reviews Bioengineering

@natrevbioeng

7 months

Delivering the CRISPR machinery into specific target cells in vivo remains challenging! The @doudna_lab have now developed cell-permeable CRISPR ribonucleoproteins that can edit cells in vivo without requiring an additional delivery vehicle @NatureComms

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Kai Chen

@kaichen_1990

5 years

Congrats to Olli on this beautiful work published in @J_A_C_S ! Substrate walking and high-throughput screening coming together allows directed evolution to rapidly expand the chemistry space of native enzymes! @francesarnold Check out our paper:

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Kai Chen

@kaichen_1990

6 years

Our work is on the cover! Enzymes are amazing and sweet!

J. Am. Chem. Soc.

@J_A_C_S

6 years

The December 5, 2018 issue of JACS is now online ~ Our cover article features work from this year's Nobel Laureate @francesarnold and her group on engineered cytochrome P450-catalyzed carbene-transfer reactions.

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Kai Chen

@kaichen_1990

2 years

Three greatest female scientists (chem Nobel laureates) together, my two deeply admired bosses @francesarnold @doudna_lab , and another role model @CarolynBertozzi ! Such an inspiration!

Frances Arnold

@francesarnold

2 years

#finally caught up with ⁦ @CarolynBertozzi ⁩ and Jennifer ⁦ @doudna_lab ⁩ #nobelweek #chemnobelles ⁦ @NobelPrize ⁩

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Kai Chen

@kaichen_1990

6 years

Without changing the protein structure, how can one amino acid mutation change the chemistries the enzymes carry out? Nice collaboration with @ExaZhang and the Hong group at Zhejiang University. Check out our most recent paper on JACS:

Alternate Heme Ligation Steers Activity and Selectivity in Engineered Cytochrome P450-Catalyzed...

We report a biocatalytic platform of engineered cytochrome P450 enzymes to carry out carbene-transfer reactions using a lactone-based carbene precursor. By simply altering the heme-ligating residue,...

pubs.acs.org

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Kai Chen

@kaichen_1990

10 months

Congrats to @AmyReginaEggers and the whole team! It is also the third story I want to share this year. We used structural and biochemical analyses to explore why our evolved Cas9 is a more effective editor and applied these insights to rationally engineer other CRISPR editors.

Amy R Eggers

@AmyReginaEggers

10 months

Today I am excited to share our preprint “Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9” from @doudna_lab , with co-authors @kaichen_1990 , Kasia Soczek, @owen_t_tuck , @Ee_Doherty , @BW_Thornton , Bryant Xu, and Marena Trinidad. 1/7

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Kai Chen

@kaichen_1990

3 years

Super hero, great scientist and role model #yuanlongping will keep shining in the human history! Still have not recovered from the grievous news of him passing away.

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Kai Chen

@kaichen_1990

4 years

Touched to see the words by ⁦⁦ @francesarnold ⁩ . Really feel #ScienceIsBack , and scientists can focus on science without worrying about too much other stuff (especially me as an international researcher). Respect for you, a great leader!

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Kai Chen

@kaichen_1990

6 years

Celebrating Frances’ Nobel Prize @francesarnold with @xiongyih @JiaZhijun . So excited to witness this moment!!!

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Kai Chen

@kaichen_1990

6 years

Frances Arnold - 2018 Nobel Prize Winner in Chemistry! Great great congrats! So proud to be her student!

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Kai Chen

@kaichen_1990

6 years

It is so honorable to contribute to the 30th anniversary issue of Synlett! Thanks to all the co-workers in this work @xiongyih @ExaZhang @sbjennykan , Andrew Zhou and of course, @francesarnold and Xin Hong!

Chemistry at Thieme

@thiemechemistry

6 years

🔝💡 It's time for the first article highlight of the SYNLETT Pearl Issue! @francesarnold , Xin Hong and their labs present an engineered cytochrome c-catalyzed lactone-Carbene B–H insertion. 😮 Check it out ➡️ #SYNLETT30

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Kai Chen

@kaichen_1990

4 years

It was my last group meeting at Caltech! #Directedevolution rocks!

Frances Arnold

@francesarnold

4 years

#Directedevolution on @novapbs ! Check this out! @Ella_Watkins_ @Sabine_BC @TheLucaccino @kaichen_1990 @natgoldberg2 #chemistry #enzymes

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Kai Chen

@kaichen_1990

6 years

Very excited to be a Resnick fellow and show how powerful enzymes can be for green chemistry development! Gratitude to @resnick_caltech for this opportunity and to @francesarnold for great support!

Frances Arnold

@francesarnold

6 years

Congratulations to our own @kaichen_1990 , the newest @resnick_caltech grad fellow! More new enzymes to come, good #greenchemistry .

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Kai Chen

@kaichen_1990

4 years

Congratulations to our @francesarnold lab undergrad @andrewzzhou5 . Great job on this project! Proud of you!

Andrew Zhou

@andrewzzhou5

4 years

Selective C–H functionalization with new P450 enzymes! #directedevolution Check out our newest paper @ACSCatalysis ! Thanks to @francesarnold and @kaichen_1990 for the guidance on this project!

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Kai Chen

@kaichen_1990

3 years

Excited to see this work accomplished by the team @zhenliuchem and @MarcGBQ led! I was so fascinated by these enzymes, but had no time to dive into and finish the project before graduation. Really striking enzymes with precise control!

Zhen

@zhenliuchem

3 years

Thanks to everyone @francesarnold @MarcGBQ @kaichen_1990 @ccalvotusell @andrewzzhou5 , our paper on P450-catalyzed asymmetric carbene N–H insertion is on ChemRixv We hope this new discovery can be applied to the development of novel biocatalytic reactions!

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Kai Chen

@kaichen_1990

11 months

In this project, we first used #directedevolution to engineer an efficient and ultra-stable CRISPR gene editor. The ultra-stability and desired charge density allowed the gene editor in ribonucleoprotein (RNP) format to be effectively encapsulated into lipid nanoparticles (LNPs).

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Kai Chen

@kaichen_1990

4 years

Big news!!! Congratulations @francesarnold !

Eric Topol

@EricTopol

4 years

Congratulations @eric_lander , @NIHDirector , @francesarnold @alondra , @maria_zuber , science leaders of the new @JoeBiden / @KamalaHarris administration. And the 1st time the top science advisor will be a Cabinet member 👍 @WHOSTP @NIH

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Kai Chen

@kaichen_1990

5 years

Every time so touched to see this video with tears... Now restrictions in Wuhan have been lifted after getting over the hardships and fighting against #coronavirus for two months. Hope the world will prevail in this battle! The lockdown: One month in Wuhan

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Kai Chen

@kaichen_1990

3 years

Great opportunity for bioinformatic people! Come work with us!

Doudna Lab

@doudna_lab

3 years

Come work with us!

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Kai Chen

@kaichen_1990

3 years

Those who have devoted their life to helping the world will be memorized, and their footprints will be followed by young scientists. (Artwork credits to the shown weibo user)

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Kai Chen

@kaichen_1990

6 years

What an amazing paper from @LongCai_Lab ! Great congratulations to my roommate Linus who accomplished the beautiful work but kept this as a secret to me🤪

Cai Lab

@LongCai_Lab

6 years

Breaking the transcriptome barrier with seqFISH+(base20x3). Evolved from temporal barcoding with seqFISH(2014,base4), tissue(2016,base5), 2017-in vitro SPOTs & 2018-intron (base12). KeyPoints: in situ discovery tool,faster in imaging,neighbors-specific GE

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Kai Chen

@kaichen_1990

2 years

@athavale_s @JACS @Shilong_Gao @Anuvab_tweet @SharathChM @alfonz___ @jenniferhirsch5 @francesarnold Congratulations Soumitra and team! This is the most exciting work in biocatalysis (or even in synthetic chemistry) I have seen this year! How far are you pushing the limit of the Arnold lab enzymes! New standard is now set up for non-natural enzymatic functions.

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Kai Chen

@kaichen_1990

4 years

@ChemicalScience Happy birthday, @ChemicalScience ! #MyfirstChemSci was also my first first-author paper @ZJU_China , back to 2013. We developed a C-H activation method to synthesize a large set of non-canonical amino acids with various side chains.

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Kai Chen

@kaichen_1990

2 years

@xiongyih @JRui7001 @qun_zhao @ajhuls1 @noelnehc @MossbauerLab @MarcGBQ Congratulations, Xiongyi and team, on the fantastic work of challenging enzymes to do crazy chemistry! “Instead of asking what enzymes do in the natural world, we can now ask, what might they do?”-Frances

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Kai Chen

@kaichen_1990

7 years

@francesarnold @xiongyih @sbjennykan It’s possible🤔🤔. Bacteria can now create what we want, just as God do😆

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Kai Chen

@kaichen_1990

3 years

袁公走好

People's Daily, China

@PDChina

3 years

Yuan Longping, a prestigious agronomist and a recipient of the Medal of the Republic, China’s highest state honor, passed away on May 22, at the age of 91.

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Kai Chen

@kaichen_1990

7 years

Have faith in your enzymes, and they will amaze you! - making strained carbocycles Credit to the whole smart team - Xiongyi, Jenny and Kelly, and the best advisor Frances!

Frances Arnold

@francesarnold

7 years

Our newest new enzyme from the one and only @kaichen_1990 . This is WILD: Enzymatic construction of highly strained carbocycles

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Kai Chen

@kaichen_1990

3 years

Great teamwork by @zhenliuchem @MarcGBQ @andrewzzhou5 and @ccalvotusell ! And huge thanks to @francesarnold for including me as one of the corresponding authors on this project!

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Kai Chen

@kaichen_1990

6 years

See, how excited and crazy I was (we were) when @francesarnold won the Nobel Prize in Chemistry!

Frances Arnold

@francesarnold

6 years

@Caltech prepared a short video of my big #NobelPrize day: A Day with Caltech's 2018 Nobel Laureate via @YouTube

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Kai Chen

@kaichen_1990

6 years

@francesarnold it’s such a great moment! Cannot get to sleep.

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Kai Chen

@kaichen_1990

3 years

@LongCai_Lab @CHLinusEng Congratulations, Dr. Eng @CHLinusEng , my five-year-long roommate at Caltech! Looking forward to more exciting science from you!

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Kai Chen

@kaichen_1990

11 months

Upon direct RNP injection into the mouse brain, the self-deliverable gene editors led to robust editing in vivo. We believe this self-delivery strategy would provide a facile and effective platform for genome editing both ex vivo and in vivo.

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Kai Chen

@kaichen_1990

11 months

Credits go to excellent collaborators for their contribution to this project, including @AbbyLabby_ , Min Kang, and many others @doudna_lab .

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Kai Chen

@kaichen_1990

11 months

@zhaoweiasu @doudna_lab Thanks for the great questions, exactly what we are planning to figure out next (cell types and immunogenicity). Base editors and prime editors will require a lot of LNP optimization if RNPs are the delivery cargos, and we only have preliminary results for now.

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Kai Chen

@kaichen_1990

4 years

@KevinKaichuang @hanzyduzit @francesarnold Berkeley🧬

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Kai Chen

@kaichen_1990

4 years

@hanzyduzit @francesarnold Thanks for the invitation, Hans! Always a lot of fun interacting with you and your great group members!

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Kai Chen

@kaichen_1990

3 years

@Stephan_Hammer @MarcGBQ Awesome work! Congrats Stephan! Really excited to see it.

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Kai Chen

@kaichen_1990

11 months

Our demonstration will offer the potential to expand the therapeutic utility of CRISPR gene editing. Credits to my collaborators, Hesong Han, Sheng Zhao, and many others in the Murthy and Doudna labs.

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Kai Chen

@kaichen_1990

4 years

@SuYapeng @CellCellPress @HutchGreenberg @raphg @kickassscience1 @ISBLeeHood @ISBNathanPrice @isbsci @Swedish @Stanford @fredhutch @UCSF @seattlechildren Congratulations! Great and important work!

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Kai Chen

@kaichen_1990

5 years

@IQCCUdG @MarcGBQ Congrats Marc!

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Kai Chen

@kaichen_1990

11 months

Robust gene editing upon RNP:LNP delivery was observed not only in cultured cells but also in vivo with mouse models. In particular, we developed different LNP formulations that could drive delivery specificity to the liver and the lungs for gene editing nearly exclusively.

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Kai Chen

@kaichen_1990

2 years

@athavale_s @uclachem Congratulations, Soumitra! It’s really fantastic news! Looking forward to your exciting research at UCLA!

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Kai Chen

@kaichen_1990

3 years

@hanzyduzit @NatProdReports Congrats, Hans!!!

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Kai Chen

@kaichen_1990

6 months

@KevinKaichuang @NatureBiotech @TrichomeDoctor @Xiaozhi_Fu @AZelezniak Congratulations, Kevin!

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Kai Chen

@kaichen_1990

3 years

@hanzyduzit @jkbcahn @NatureChemistry Well well I also don’t know 😂

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Kai Chen

@kaichen_1990

6 years

Evolution made it possible!

Tina Boville

@TinaBoville

6 years

@francesarnold it looks like your feet grew this week!

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Kai Chen

@kaichen_1990

8 months

@Dylan_Smock @NatureComms @doudna_lab Thanks Dylan!

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Kai Chen

@kaichen_1990

3 years

@KevinKaichuang Nope as far as I know here😂 but do remember we got hundreds of crispr mugs as gifts from some company

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Kai Chen

@kaichen_1990

4 years

@Stephan_Hammer Congratulations, Stephan!

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Kai Chen

@kaichen_1990

2 years

@xinhong_chen Congratulations, Xinhong!

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Kai Chen

@kaichen_1990

2 years

@SuYapeng @DamonRunyon @HutchGreenberg @raphg @LabHeath @fredhutch @isbsci @Caltech Huge congrats 🎉 @SuYapeng

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Kai Chen

@kaichen_1990

4 years

@KevinKaichuang 🤣it’s true...the whole office since your time is all gone now...even did not think about it

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Kai Chen

@kaichen_1990

8 months

@SuYapeng @NatureComms @doudna_lab Thanks Yapeng!

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Kai Chen

@kaichen_1990

11 months

@Dylan_Smock @doudna_lab Thanks Dylan!

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Kai Chen

@kaichen_1990

2 years

@GuanyaShi @CarnegieMellon @CMU_Robotics @SCSatCMU @yisongyue Congratulations Guanya!

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Kai Chen

@kaichen_1990

4 years

@MarcGBQ @AgEInves @CienciaGob @UdGRecerca @IQCCUdG Congratulations, Marc!

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Kai Chen

@kaichen_1990

4 years

@UCB_Chemistry @DodaniLab Congratulations @sheeldodani !

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Kai Chen

@kaichen_1990

5 years

@athavale_s @NicholasSarai Congrats, Soumitra!

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Kai Chen

@kaichen_1990

5 months

@SuYapeng @CellCellPress Thank you Yapeng!

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Kai Chen

@kaichen_1990

4 years

@KevinKaichuang 新年快乐🎆🎆🎆

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Kai Chen

@kaichen_1990

11 months

@SuYapeng @francesarnold @doudna_lab Thanks Yapeng!

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Kai Chen

@kaichen_1990

5 years

@SuYapeng @francesarnold Thanks, Yapeng.

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Kai Chen

@kaichen_1990

5 years

@hanzyduzit @francesarnold Thanks, Hans! Of course, we did a lot previously to derivatize terminal cyclopropenes to multi-substituted cyclopropanes and also to open the triangle ring. I believe the internal ones should also work.

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Kai Chen

@kaichen_1990

2 years

@CindySE27 I told you! Once you get started at Stanford, you will be working in another Nobel laureate’s lab😜 Congrats!🎉

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Kai Chen

@kaichen_1990

4 years