Savage Lab @SavageCatsOnly Twitter profile

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Savage Lab

6 months

What limits rubisco function? Is it the chemical mechanism? Evolution? In an updated pre-print @prywes et. al explore this question by assaying >99% of single amino acid mutants in Form II rubisco (1/7)

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Savage Lab

@SavageCatsOnly

5 years

From our lab to yours, Happy Halloween! May your 260/280 always be ~1.8 and your 260/230 fall within 2.0-2.2 🎃🧬🧪 @QIAGEN

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Savage Lab

@SavageCatsOnly

3 years

🚨 New pre-print! We engineered catalytically inactive and improved #Cas13 effectors for efficient post-transcriptional regulation of genes in bacteria and human cells. 1/5

Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression

Cas13 is a family of unique RNA-targeting CRISPR-Cas effectors, making it an appealing tool for probing and perturbing RNA function. However only a few Cas13 homologs have been shown to mediate...

www.biorxiv.org

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Savage Lab

@SavageCatsOnly

6 months

Former Amgen scholar @caroguerrero522 enjoyed her summer in the Savage lab so much she decided to come back for more. Excited to welcome this Savage Cat back to the lab as a grad student!

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Savage Lab

@SavageCatsOnly

3 years

New collabo with @theJensenLab out now!!

Jensen Lab

@theJensenLab

3 years

Check out our new @biorxivpreprint with @SavageCatsOnly on RuBisCO lattices in α-carboxysomes:

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Savage Lab

@SavageCatsOnly

4 years

Years in the making - a peroxidase-encapsulating nanocompartment helps M. TB survive oxidative stress in acidic environments including in hosts! Amazing collaboration between Katie Lien, @Nichols_NTA and Caleb CA, #SavageCats and Stanley Lab.

A nanocompartment containing the peroxidase DypB contributes to defense against oxidative stress in...

Encapsulin nanocompartments are an emerging class of prokaryotic protein-based organelles consisting of an encapsulin protein shell that encloses a protein cargo[1][1]. Genes encoding nanocompartme...

www.biorxiv.org

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Savage Lab

@SavageCatsOnly

3 years

Our new paper c/o first authors @realarikshams & Sean Higgins is LIVE !! We report on engineering minimal Cas9 enzymes for maximum impact 🧬✂️

Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

Nature Communications - Proteins evolve through the modular rearrangement of domains. Here the authors introduce MISER, a minimization by iterative size-exclusion and recombination method to make...

www.nature.com

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Savage Lab

@SavageCatsOnly

6 years

New work out from Savage Cats! “CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification” congrats to Ben, Christoff, Harneet, Kian, Rayka et al!!!!

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Savage Lab

@SavageCatsOnly

4 years

Please RT! We’re looking to hire a postdoc interested in CRISPR biochemistry with a focus on diagnostics! Interested candidates can DM this account or email savage @berkeley .edu with a CV & cover letter.

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Savage Lab

@SavageCatsOnly

4 years

. @Nichols_NTA 's paper on a novel prokaryotic nanocompartment is out on @eLife 🤙! Check out the final version of Rob's thesis research on #encapsulins and #cyanobacteria below 👇 Special thanks to our amazing collaborators @bjlafrance and @NogalesLab !

Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur...

A protein-bounded organelle-like compartment from Synechococcus elongatus PCC 7942 is upregulated under sulfur starvation and contains a cysteine desulfurase cargo enzyme.

elifesciences.org

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Savage Lab

@SavageCatsOnly

5 months

🥳🥳🥳

UC Berkeley MCB

@berkeleyMCB

5 months

Congrats to MCB's David Savage @SavageCatsOnly on his promotion to Professor of Biochemistry, Biophysics and Structural Biology! 👏🥂

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Savage Lab

@SavageCatsOnly

3 years

Like and RT this tweet to congratulate Savage Cat @BW_Thornton for passing her qualifying exam and ascending to PhD Candidate! @berkeleyMCB 🥳

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Savage Lab

@SavageCatsOnly

4 years

Our #preprint on MISER, a new technique for minimizing proteins is up on @biorxivpreprint ! Tweetsplainer thread by @realarikshams below, including some neat insights on #CRISPR -Cas9 ⬇️

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Savage Lab

@SavageCatsOnly

6 months

read the whole thing here!

A map of the rubisco biochemical landscape

Rubisco is the primary CO2 fixing enzyme of the biosphere yet has slow kinetics. The roles of evolution and chemical mechanism in constraining the sequence landscape of rubisco remain debated. In...

www.biorxiv.org

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Savage Lab

@SavageCatsOnly

3 years

good data is the best high 🤙📈

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Savage Lab

@SavageCatsOnly

5 years

“Onwards and upwards” to a nap!!!

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Savage Lab

@SavageCatsOnly

3 years

the lab is in shambles rn

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Savage Lab

@SavageCatsOnly

3 years

We’ve had much cause to celebrate lately, including bidding a bittersweet farewell to Eli Dugan, our amazing lab manager who is starting his PhD at @UCSF Tetrad in the Fall! 🥳 Also 👏🏾 to our commander-in-chief #Dave ( @dfsavage ) for TEN YEARS at @UCBerkeley 😳😮‍💨🔟

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Savage Lab

@SavageCatsOnly

4 years

Welcome to our newest Savage Cats, Brittney Thornton ( @BW_Thornton ) and Julia Tartaglia ( @juliaamber10 )! Look for great things to come 👩‍🔬🧬 @berkeleyMCB

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Savage Lab

@SavageCatsOnly

5 years

Big congrats to Luke and Benz on the culmination of untold hours scrutinizing csos2! Now we finally understand how this strange disordered repeat protein brings rubisco to the carboxysome. #savagecats #upwardsandonwards

Multivalent interactions between CsoS2 and Rubisco mediate α-carboxysome formation

Nature Structural & Molecular Biology - Structural and binding studies show that a repeated peptide motif in the N-terminal domain of CsoS2 mediates multivalent interactions with assembled...

www.nature.com

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Savage Lab

@SavageCatsOnly

5 years

Congratulations to @Nichols_NTA for Best Grad Student Talk at the @berkeleyMCB BBS retreat! 🦠

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Savage Lab

@SavageCatsOnly

5 years

Some #savage #whiteboardwisdom for the new week y’all

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Savage Lab

@SavageCatsOnly

2 years

Our newest Cats have the #synbio community SHOOK! We’re thrilled to welcome @florazhiqiwang , @rachelfweissman , and @rodriguezje1991 to the lab!

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Savage Lab

@SavageCatsOnly

6 years

Introducing the #Savagest BoyBand #42degrees

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Savage Lab

@SavageCatsOnly

3 years

Here’s the tweet you’ve all been waiting for: We’re thrilled to welcome @berkeleyMCB grad student Muntathar Al-Shimary to the lab! 🧬🧑‍🔬😼

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Savage Lab

@SavageCatsOnly

3 years

We are looking to hire a Science Research Associate to work with us at the interface of protein design + CRISPR/Cas + applied gene editing! Please share broadly!

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Savage Lab

@SavageCatsOnly

6 months

Rubisco is, after all, an enzyme, so we wanted to biochemically characterize each mutation. By repeating the assay over CO2 titration, key biochemical parameters (e.g. Vmax, Kc) are estimated for each mutant! (5/7)

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Savage Lab

@SavageCatsOnly

4 years

Huge congrats to our very own Rob Nichols @Nichols_NTA - just posted his paper on the discovery and characterization of a whole new sulfur-metabolizing branch of the bacterial nanocompartment family - "family 2 encapsulins." Rewarding collabo with @bjlafrance and @NogalesLab

Bobby Digit4L Nichols

@Nichols_NTA

4 years

So happy to see our pre-print online!! We discovered an characterized a new prokaryotic organelle - a novel family of encapsulins! With the help of @bjlafrance and the @NogalesLab we determined the structure of the compartment to 2.2 Angstrom resolution!!

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Savage Lab

@SavageCatsOnly

4 years

Incredibly proud of our collaborator, @igisci neighbor, and @berkeleyMCB faculty Jennifer Doudna @doudna_lab for this historic achievement! #CRISPR #WomenInSTEM #GoBears

The Nobel Prize

@NobelPrize

4 years

BREAKING NEWS: The 2020 #NobelPrize in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”

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Savage Lab

@SavageCatsOnly

3 years

top o’ the morning, top o’ the morning, top o’ the morning, top o’ the morning, top o’ the morning, top o’ the morning, top o’ the morning

HHMI

@HHMINEWS

3 years

HHMI News: We are proud to announce our 33 new #HHMIInvestigators . These individuals have the potential to make transformative discoveries over time and we look forward to seeing where their ideas lead them.

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Savage Lab

@SavageCatsOnly

6 years

Julia’s qualsified! Come party w us and enjoy some of her EtOH. 4th floor EBB.

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Savage Lab

@SavageCatsOnly

5 years

It’s been a big year for E. coli - she graduated from heterotrophy school and has been learning a lot of new metabolisms! Congrats to Bar-Even lab on the culmination of many years of work!!

Growth of E. coli on formate and methanol via the reductive glycine pathway

Nature Chemical Biology - Redesigning the central carbon metabolism of Escherichia coli with the reductive glycine pathway enables growth on the one-carbon compounds formate and CO2, and the...

www.nature.com

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Savage Lab

@SavageCatsOnly

4 years

Resident baller @Nichols_NTA gives his thesis research presentation to start off this year’s virtual MCB TheRPs. Great talk Rob!

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Savage Lab

@SavageCatsOnly

7 months

Qualified✅ NSF GRFP ✅ Join us in congratulating PhD candidate @CynthiaTerrace on passing her qualifying and being awarded the NSF GRFP! 🎉 🎉🎉

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Savage Lab

@SavageCatsOnly

5 years

A huge congrats to Sean and @flamholz ! Onwards and upwards!!

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Savage Lab

@SavageCatsOnly

1 year

Three brand new savage cats ready to hit the lab. We're thrilled to welcome @CynthiaTerrace (and her feline friends) to the Savage lab! 🥽🥼🧪🙀

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Savage Lab

@SavageCatsOnly

5 years

Behind ⁦ @Jjdesmarais2 ⁩ new hot off the presses paper: DAB family transporters are everywhere in prokaryotes!

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Savage Lab

@SavageCatsOnly

4 years

Every day in the Savage Lab we ask ourselves, "what's new with #carboxysome engineering?" If you find yourself new to this line of thinking, fear not! Our own @JuliaBorden has authored a #review on the topic for your reading pleasure! 👇 #synbiothoughts

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Savage Lab

@SavageCatsOnly

1 year

But wait, there's more! New work by Turnšek et al. identifies key residues that drive this assembly, shows the effects of mutation in vivo, and probes the biochemistry in vitro with reconstituted carboxysomes!

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Savage Lab

@SavageCatsOnly

2 years

Congrats to our Certified Fresh™️ PhD candidate Muntathar Al-Shimary on #crushing his qualifying exam! @berkeleyMCB @doudna_lab

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Savage Lab

@SavageCatsOnly

4 years

Big week for #synbio at the Savage Lab!! @flamholz , Eli, @cblikstad , and @MiloLabWIS ’s paper on reconstituting a CO2 concentrating mechanism in E. coli is out in @eLife ! 🦠

Avi Flamholz

@flamholz

4 years

It's alive!!!

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Savage Lab

@SavageCatsOnly

1 year

In the first preprint of the weekend, @loltrogge et al. find that the size of α-carboxysomes is strongly specified by CsoS2 and, in particular, the relative abundance of M- and C-type peptide repeats. ⬇️

α-carboxysome size is controlled by the disordered scaffold protein CsoS2

Carboxysomes are protein microcompartments that function in the bacterial CO2 concentrating mechanism (CCM) to facilitate CO2 assimilation. To do so, carboxysomes assemble from thousands of constit...

www.biorxiv.org

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Savage Lab

@SavageCatsOnly

6 months

Small changes in sequence can have big consequences in function, and not only negatively! We hope that this data is useful to other enzyme engineers and that this result expands our view of what is possible with even small changes. (7/7)

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Savage Lab

@SavageCatsOnly

4 years

The year of the encapsulin CONTINUES with our new paper w/ @bjlafrance , Caleb Cassidy-Amstutz, and @NogalesLab . We show that a nanocompartment shell binds flavin, suggesting it could participate in redox chemistry... This one is HOT- call it Thermatoga!

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Savage Lab

@SavageCatsOnly

5 years

. @Flamholz ’s heartfelt farewell address to the lab. “Eat the pizza” —Avi Flamholz, PhD.

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Savage Lab

@SavageCatsOnly

6 months

To address this, @prywes et al developed an assay to estimate the fitness of 99% of single mutations in a high-throughput E. coli screen. E. coli growth rate is linked to enzyme behavior! (3/7)

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Savage Lab

@SavageCatsOnly

4 years

Things our beloved postdoc @cblikstad will miss about California, in ascending order: - the CA sea and sun - working with CArboxysomes - her wonderful labmates, the Savage CAts Please join us in wishing Cissi well as she starts her own group in @chem_angstrom at @uppsalauni ! 🧑‍🔬

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Savage Lab

@SavageCatsOnly

2 years

🌼 𝓕𝓵𝓸𝓻𝓪𝓵 𝓕𝓻𝓲𝓭𝓪𝔂 🌼 at the Savage Lab 🌹🌸🌷🌺🐱🌻💐🌼🪷

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Savage Lab

@SavageCatsOnly

1 year

A light ☀️ read on a dark 🕶️ reaction enzyme. Everything you want to know about “one of the most pivotal enzymes to life on earth”. Brought to you by @NoamPrywes @NaiyaPhillips @owen_t_tuck @luisarchaeota Read it now @AnnualReviews ! 😍📖🔥

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Savage Lab

@SavageCatsOnly

5 years

Exciting new work by our labmate Luke Oltrogge et alia: dissection of the mechanism of csos2-mediated recruitment of Rubisco into the alpha carboxysome.

α-carboxysome formation is mediated by the multivalent and disordered protein CsoS2

Carboxysomes are bacterial microcompartments that function as the centerpiece of the bacterial CO2-concentrating mechanism, feeding high concentrations of CO2 to the enzyme Rubisco for fixation. The...

www.biorxiv.org

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Savage Lab

@SavageCatsOnly

3 years

Another day, another reason to celebrate in our lab. Congratulations to Savage Cat (and now PhD candidate) @juliaamber10 for passing her quals! 🥳🍾👩‍🔬

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Savage Lab

@SavageCatsOnly

6 months

As the entry-point for nearly all CO2 to the biosphere, rubisco is arguably one of the most important enzymes. Yet, Rubisco catalysis is slow. While there are 100s of biochemical measurements, our understanding of catalysis is limited by low-throughput in vitro assays (2/7)

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Savage Lab

@SavageCatsOnly

6 months

Several single AA mutations increased the CO2 affinity! Given sequence divergence and different oligomeric states of Form I & II, we were surprised to find single muts led to CO2 affinity outside of the range of Form II & at the edge of the distribution of plants and algae (6/7)

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Savage Lab

@SavageCatsOnly

5 years

Big congrats to our friends in the Milo Lab @WeizmannScience on publishing this huge achievement: "Conversion of Escherichia coli to Generate All Biomass Carbon from CO2"

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Savage Lab

@SavageCatsOnly

6 months

This assay neatly identified known structural, functional, and evolutionary effects, confirming the robustness of the screen. For example, key residues in Loop 6 and the active site, are mutation-intolerant (4/7)

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Savage Lab

@SavageCatsOnly

2 years

A bittersweet and fond farewell to our lab manager Shin Kim, as he embarks on his med school journey at UCLA!

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Savage Lab

@SavageCatsOnly

2 years

Our talented technician, Emily Meier ( @emileedle_ ), is off to @HopkinsCMDB for her PhD! Emily has been a wonderful and valuable member of the lab and the diagnostics team, and we’re excited for her adventures in Baltimore! 👩‍🔬🧬✌️

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Savage Lab

@SavageCatsOnly

3 years

Dave breaks a 10 year Twitter silence to shout out #woodgang 🌱🌳🪵

Dave Savage

@dfsavage

3 years

Planted 70 trees to celebrate dads ~70th at the home place in Iowa. Does this count for #chalkitdown ? @SavageCatsOnly

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Savage Lab

@SavageCatsOnly

6 years

Congrats Emeric on passing his qualifying exam!!!!!

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Savage Lab

@SavageCatsOnly

3 years

“what I cannot create, I do not understand. what I cannot understand, I must destroy.”

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Savage Lab

@SavageCatsOnly

6 years

Everyone should read this fabulous paper from Manajit Hayer-Hartl’s lab. We saw her speak about it recently and it is phenomenal work that explains a lot about beta carboxysome formation.

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Savage Lab

@SavageCatsOnly

3 years

Wowww, carboxysomes? 😯 Carbonic anhydrases?? 😮‍💨 RUBISCO??? 🤯 This preprint from Savage Cat @cblikstad (now Group Leader at @UU_University ) has it all!! Check out the thread below for an explainer. Congrats Cissi et alia! 🦠

Cecilia Blikstad

@cblikstad

3 years

Happy to share that our work on how carbonic anhydrase encapsulates into α-carboxysomes is now on Biorxiv. Tl;dr: We discovered a carbonic anhydrase-Rubisco supercomplex and solved a sub 2 Å cryoEM structure. @SavageCatsOnly @berkeleyMCB 1/

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Savage Lab

@SavageCatsOnly

4 years

BREAKING: Jason Derulo has fallen down the stairs at the remote BBS retreat on zoom

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Savage Lab

@SavageCatsOnly

4 years

🆒🆕 Super cool work by @patrick_pausch et. al. on the characterization of a new CRISPR protein, CasΦ, that can cut dsDNA despite being ~half the size of Cas9! 🧬✂️

Patrick Pausch

@patrick_pausch

4 years

Very excited to share my first postdoc paper. We experimentally characterized the hypercompact CRISPR-CasΦ system (type V-J). At half the size of Cas9, CasΦ is still able to cut dsDNA. Great collaboration with @themicrobeguy , @ScienceMagazine (1/6)

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Savage Lab

@SavageCatsOnly

6 years

Savage cats do Halloween. Costumes include: unicorn pusheen, triceratops, @scotusginsburg does science, and quite possibly the best Mario and Luigi you will ever see IRL!!! 🦄🐱🦖👩‍⚖️👨🏻‍🔧👨🏻‍🔧

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Savage Lab

@SavageCatsOnly

2 years

Whew, we've got a double-whammy preprint drop for you to peruse this weekend! @flamholz & Eli et al, along with Renee @Caltech GPS & @Nichols_NTA has a step-by-step guide on how to develop a superpower ⁽ᶦⁿ ᵇᵃᶜᵗᵉʳᶦᵃ⁾ 🦠💪⚡️ 1/3

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Savage Lab

@SavageCatsOnly

6 years

New work out by #SavageCat ⁦ @3rdFloorTom ⁩ - Oblong structure of the complex I-like molecule NDH of oxygenic photosynthesis | Nature

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Savage Lab

@SavageCatsOnly

4 years

Thrilled to see what @scribetx (co-founded by Savage Lab alum @BenjaminLOakes ) has in store for the #CRISPR therapeutics space!

Scribe Therapeutics

@scribetx

4 years

1/ We are excited to take the wraps off Scribe Therapeutics - a molecular engineering company focused on building best-in-class in vivo therapies to permanently treat the underlying cause of disease. Read our launch press release:

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Savage Lab

@SavageCatsOnly

5 years

“Revisiting Trade-offs between Rubisco Kinetic Parameters” v5.0 is officially published in ⁦ @BiochemistryACS ⁩

Revisiting Trade-offs between Rubisco Kinetic Parameters

Rubisco is the primary carboxylase of the Calvin cycle, the most abundant enzyme in the biosphere, and one of the best-characterized enzymes. On the basis of correlations between Rubisco kinetic...

pubs.acs.org

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Savage Lab

@SavageCatsOnly

4 years

Congrats to Rob on a great PhD!!! 🍻🍻Rob set out to answer some of the great mysteries of the prokaryotic nanocompartments (AKA encapsulins) for his dissertation. Turns out there are a bunch of these systems and there are even more questions/ mysteries now!

Bobby Digit4L Nichols

@Nichols_NTA

4 years

Dr. Robert Nichols 🍾🤙

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Savage Lab

@SavageCatsOnly

5 years

Savagest beach day. Tough life out here.

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Savage Lab

@SavageCatsOnly

11 months

What controls the size of α-carboxysomes?? Click the link below⬇️ to find out NOW!

α-Carboxysome Size Is Controlled by the Disordered Scaffold Protein CsoS2

Carboxysomes are protein microcompartments that function in the bacterial CO2 concentrating mechanism (CCM) to facilitate CO2 assimilation. To do so, carboxysomes assemble from thousands of constit...

pubs.acs.org

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Savage Lab

@SavageCatsOnly

6 years

Pretty #Savage perspective out in Biochemistry describing recent work by Manajit Hayer-Hartl's lab showing that liquid-liquid phase separation of rubisco+ccmM underlies the formation of the beta carboxysome. Wise words by @cblikstad @flamholz and Luke

Learning to Build a β-Carboxysome

pubs.acs.org

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Savage Lab

@SavageCatsOnly

4 years

Congratulations to @flamholz , Eli, @cblikstad and @MiloLabWIS collaborators - this paper is the culmination of many years of work, and many decades of collective effort studying bacterial CO2 concentrating mechanisms. So exciting to see it in the world!

Avi Flamholz

@flamholz

4 years

So happy that our new manuscript "Functional reconstitution of a bacterial CO2 concentrating mechanism in E. coli" is up on @bioRxiv

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Savage Lab

@SavageCatsOnly

3 years

Amazing work by @JoshCofsky of @doudna_lab figuring out how exactly DNA is interrogated by Cas9! If only we had this for all the engineered variants out there… 👀😮‍💨

Josh Cofsky

@JoshCofsky

3 years

New pre-print! While #CRISPR -Cas9 searches through the genome, how does it actually "read" DNA sequence information, which is normally hidden within the double helix? We determined structures of Cas9 in the act of DNA interrogation to find out! Thread below 👇 (1/8)

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Savage Lab

@SavageCatsOnly

5 years

Important application of CRISPR tech - isothermal 10 min detection of #covid19 . @mammothbiosci @igisci

Mammoth Biosciences

@mammothbiosci

5 years

We've updated our white paper, "A protocol for rapid detection of SARS-CoV-2 using CRISPR: SARS-CoV-2 DETECTR." Check it out to learn how our DETECTR platform could be used to detect SARS-CoV-2 #COVID ー19 #COVID19 #CRISPR #Diagnostics #CRISPRDx

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Savage Lab

@SavageCatsOnly

6 years

Congrats to Daniella and the Merchant lab on publishing this amazingly comprehensive view of Chlamydomonas physiology and gene expression

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Savage Lab

@SavageCatsOnly

2 years

👑 Capping off the end of a successful summer CRISPRing plants with @AmgenFoundation Scholar Carolina Guerrero! Look out for her next year in a PhD program near you 👀 #amgenscholars 🌱

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Savage Lab

@SavageCatsOnly

5 years

Luke and Benz's new paper on the intrinsically disordered repeat protein, Csos2, that brings the carboxysome together; a summary thread!

Multivalent interactions between CsoS2 and Rubisco mediate α-carboxysome formation

Nature Structural & Molecular Biology - Structural and binding studies show that a repeated peptide motif in the N-terminal domain of CsoS2 mediates multivalent interactions with assembled...

www.nature.com

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Savage Lab

@SavageCatsOnly

1 year

Back at it with another double preprint drop 📝📝Starring the repetitive, disordered, and “mysterious” scaffold protein CsoS2 and its role in the α-carbozysome. Two (Twitter) threads incoming! 🤩

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Savage Lab

@SavageCatsOnly

5 years

Here's the updated preprint from @mammothbiosci

Mammoth Biosciences

@mammothbiosci

5 years

Read our @medrxivpreprint co-authored with @cychiu98 ’s lab @UCSF to see how our #CRISPR -based DETECTR lateral flow assay can precisely detect SARS-CoV-2 in real patient samples! #COVID19 #CoronaVirus

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Savage Lab

@SavageCatsOnly

6 years

After the climate apocalypse, when Earth atmosphere is all reduced again, Rubisco gonna party like its 3.2 bya.

Fossilized Word of the Day

@KingLabWOTD

6 years

Today, @SavageCatsOnly would like to remind you: quantity =/= quality. @Choano_Lab might have this Twitter the rest of the year, but we only need one week 💁‍♀️💁‍♂️. RuBisCO™️: trade name for the worst-tasting yet most abundant rhubarb-flavored cookies on the planet.

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Savage Lab

@SavageCatsOnly

2 years

Woohoo! Now that it’s twitter-official, we can post this video from Maria’s celebration from >a month ago 🍾

Maria Lukarska

@MariaLukarska

2 years

Very honored that I have been awarded the @LSRFdtn postdoctoral fellowship! I'm really happy to join this community and grateful for my amazing lab @SavageCatsOnly and my mentor @dfsavage for their continued support!

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Savage Lab

@SavageCatsOnly

6 years

We find that our Monday experiments consistently run a little smoother when bacon, eggs, and mimosas are added to the reaction mixture 🤙

Fossilized Word of the Day

@KingLabWOTD

6 years

#SavageWeek is over! As @KingLabWOTD reverts back to its steady-state next week, we'll be honoring the @SavageCatsOnly tradition of Mimosa Mondays 🥂 with 96-well-format DIP. DIP-seq (n.): (alt. "dip-seek") A high-throughput strategy to locate the best dip for group meetings.

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Savage Lab

@SavageCatsOnly

3 years

I’m outside with the PCC, right outside, 6803

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Savage Lab

@SavageCatsOnly

6 years

Onwards and upwards Seanathan safety officer and protein engineer supreme! #graduation #savagecats

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Savage Lab

@SavageCatsOnly

3 years

Esteemed alum @3rdFloorTom making moves !! 💪👀

Life Sciences Research Foundation

@LSRFdtn

3 years

LSRF is pleased to announce our 2021 class of awardees! We thank our outstanding team of reviewers and, of course, the organizations and individuals providing the funding for these researchers. Congratulations! #BasicResearch , #LifeScience , #PostdocFundin …

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Savage Lab

@SavageCatsOnly

5 years

Finally a structure of the cyanobacterial CO2 uptake complex! For those who don't know, cyanos can take up both CO2 and HCO3-, but the CO2 uptake mechanism is complex and mysterious. Amazing contribution to the CCM field. Congrats to @SchullerJm @TheKailaLab @NowaLab et alia.

VilleKaila

@TheKailaLab

5 years

We proudly present how cyanobacteria efficiently concentrate CO2 from the atmosphere with the remarkable photosynthetic complex I. Great collaboration with @NowaLab and @SchullerJm . @NatureComms @ERC_Research @2017Kaw100 @Stockholm_Uni

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Savage Lab

@SavageCatsOnly

5 years

Important lessons on the history of the CRISPR revolution by one of our own @Nichols_NTA 🧬

Bobby Digit4L Nichols

@Nichols_NTA

5 years

I made a video about the CRISPR patent dispute. Parody of "Obedient Servant" from the musical Hamilton by Lin-Manuel Miranda.

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Savage Lab

@SavageCatsOnly

6 years

Mega congrats to our very own ⁦ @nicholsr2528 ⁩ on his lifetime #FolliesAchievement award jawn.

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Savage Lab

@SavageCatsOnly

6 years

Great BBS retreat this year @berkeleyMCB - excellent talks and posters, beautiful beaches, awesome food and people. Onwards and upwards!

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Savage Lab

@SavageCatsOnly

6 years

If you like our work on cas9, just wait for our work on the enigmatic #cas22b it’s #13b better! #synbio #incrementalism #wejustmakethesenamesup

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Savage Lab

@SavageCatsOnly

2 years

It’s time to 🌿BeReal🌿

Noam Prywes

@NoamPrywes

2 years

Here at @SavageCatsOnly we are thrilled to present a review on rubisco. We focused on structure, mechanism, evolution and engineering attempts. We try, and fail, to conclusively answer the question "What exactly is holding this enzyme back?" 1/10

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Savage Lab

@SavageCatsOnly

6 years

FPbase is inspiring, super useful and not to be confused with fbpase, metabolism friends!

Talley Lambert

@TalleyJLambert

6 years

@FPbase is published in @naturemethods ! So very thrilled to see what started as a fun side project get released today in one of my favorite journals. Thank you @rita_strack for the encouragement.

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Savage Lab

@SavageCatsOnly

8 years

Congrats to Dana and Stacy! Check out their new paper on making metabolite biosensors!

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Savage Lab

@SavageCatsOnly

6 years

#SavageCats #MimosaMondays

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Savage Lab

@SavageCatsOnly

5 years

When our boy @evan_groover isn’t busy enough transforming plants he’s on the sax

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Savage Lab

@SavageCatsOnly

4 years

Just out from @3rdFloorTom - a really clear and comprehensive review of recent structures of photosynthetic NADH dehydrogenase-like complexes (NDH-1). Super clear figures and explanation of structure/function relationship for plants/cyanos/algae.

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Savage Lab

@SavageCatsOnly

6 years

Need a quick primer on #scicomm and #sciencetwitter ? You probably do. Our own @JuliaBorden wrote a Toolbox for the latest edition of @BerkeleySciRev ! Pick your copy up today! 🤙🤓 (We won’t comment on the fact that this account is not on the “suggested people to follow” list)

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Savage Lab

@SavageCatsOnly

5 years

#SavageCats aspire to celebrate all the news year. #ShanaTova

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