Cristina Paulino @CPaulino_ Twitter profile

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Cristina Paulino

2 years

🥳big news🥳: 🇳🇱🔜🇩🇪The Paulino lab will be moving soon. I have been recruited as full professor to join the biochemistry centre (BZH) at the Heidelberg University and strengthen the HDcryoNet #cryoEM platform.

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Cristina Paulino

@CPaulino_

2 years

Official now: I have been promoted to tenured Associate Professor 🥳👩🏻‍🎓🤩 Wouldn’t have been possible w/o my fantastic group, the supportive environment @ResearchGbb @MembraneEnzymo1 and my mentors. #cryoem #WomenInSTEM Unofficial early mimosa 🥂🍾 celebration a few weeks ago😁

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Cristina Paulino

@CPaulino_

5 years

My first big scientific prize 🏆 super thrilled :)

2020 NVBMB prize awarded to Dr. Cristina Paulino

The 2020 NVBMB prize has been awarded to Dr. Cristina Paulino of the University of Groningen. Cristina Paulino will organize the 2020 NVBMB fall meeting. Check our site for updates.

nvbmb.kncv.nl

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Cristina Paulino

@CPaulino_

11 months

1/10 With great pride I present our newest #CryoEM work pub. in @Nature . The 1st reported secondary-active transporter directly activated by membrane voltage. Full credit to the amazing @VKalienkova , @MartinPeter91 & @rheinbergerj 🧵👇🏽 for more info

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Cristina Paulino

@CPaulino_

2 years

Check out our newest review on #cryoEM (of membrane proteins) at 200 keV. Take home message: don’t treat your 200keV TEM only as a screening machine 😉. Fantastic work by @chancie_t and @rheinbergerj 👇🏼

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Cristina Paulino

@CPaulino_

15 days

Two open fully funded Postdoc positions in my lab in #cryoEM & #cryoET . See description below 👇Please share and spread the word. For all those interested in joining my group and work on exciting and collaborative projects please apply!

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Cristina Paulino

@CPaulino_

2 years

@Lynn_Nothegger Another German word I lack an equivalent translation that encompasses the complexity and descriptiveness of the word is „Fingerspitzengefühl“

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Cristina Paulino

@CPaulino_

2 years

today was a special day🤩...

Cristina Paulino on LinkedIn: #arbeiterklasse #migrationshintergrund #frauinmint #womeninstem… |...

(English below) Ich habe heute meine Beamtenurkunde im Zuge meines Rufes zur W3 Professorin an die Universität Heidelberg erhalten und stehe damit im Dienste… | 109 comments on LinkedIn

www.linkedin.com

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Cristina Paulino

@CPaulino_

4 years

Inspired by what I saw many colleagues doing. Let's be honest about how our work-life balance often really looks like during a pandemic. Lets reveal the truth of 2020: work-headshot vs reality after working till 5am for a grant🧐😂

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Cristina Paulino

@CPaulino_

4 years

Recognizing another breakthrough in cryo-EM. I am, as always, thrilled to be an expert & part of this amazing community, even before it being so popular. We have come a long way from being pitied & called blobologists 💪🏼🤓Are we now atomists 😅What’s next?

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Cristina Paulino

@CPaulino_

3 years

Dear #cryoEM and membrane protein lovers. I have a new vacancy in my group to work on the osmoregulatory ABC transporter OpuA. Why not join us ;) Do you know of anyone? Please RT🔁 Details in the thread 👇

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Cristina Paulino

@CPaulino_

2 years

So proud 🤩 my 1st PhD student and 2nd to defend from my group. Great and successful PhD, all while becoming a mother of two!! Well done Lisa 👩🏼‍🎓!! A deserved eternal golden trophy 🏆🔬And thanks for the best present ever: a pair of 🇳🇱Dutch wooden shoes with the KdpFABC structure!

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Cristina Paulino

@CPaulino_

3 years

Proudest PI and mentor ever 🤩 Today my first PhD @VanessaClericoM graduated. What a fantastic job you did!! Can't believe it is already 4 years ago you started in my group. Thanks for letting me be part of your journey.👩🏻‍🎓 They grow out so fast 🥲.....

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Cristina Paulino

@CPaulino_

4 years

Thrilled to share our newest work on the osmoregulatory ABC transporter OpUA. #CryoEM , #smFRET and functional assays reveal a dual break mechanism of regulation, which prevents cells from exploding. Fantastic work of @HendrikSikkema and @MembraneEnzymo1

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Cristina Paulino

@CPaulino_

5 years

Our newest work is out! Structure of the human ASCT2 shows the 1st ever inward-open structure of a SLC1A (glutamate transporter). After all they work by a One-Gate-Elevator-Mechanism! Check our trial to express it in a photo :D thanks @Alisa , Dirk & Albert

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Cristina Paulino

@CPaulino_

7 years

Super proud of my first Nature paper :) Just to realise that it went back-to-back with Yifan Cheng. Hopefully many more to come soon... Activation mechanism of the calcium-activated chloride channel TMEM16A revealed by cryo-EM

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Cristina Paulino

@CPaulino_

2 years

Having a good time at the #3DEMGRC with a great drink, great location and even better company @kudryashev_lab @mattiadanza @Bex_16 @luizammend @joachimfrank so great to be back at conferences in person!

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Cristina Paulino

@CPaulino_

5 years

PhD position in my lab available! 🎉 Apply and join my super duper team! 👩🏻‍💻👨🏽‍🔬👩🏻‍🏫👨🏼‍💻👩🏻‍🎓. Pls RT

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Cristina Paulino

@CPaulino_

5 months

🤩🤩🤩 our work made it to the @NatureSMB cover. And then for such a great issue 🤩🤩 thanks to @VKalienkova @TimLynagh and Mowgli 🥳🥳 check @KatCiazynska comments 👇🏼

Kat Ciazynska

@KatCiazynska

5 months

in case you all like membrane proteins, there's a lot of cool stuff in the fresh-off the press @NatureSMB issue

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Cristina Paulino

@CPaulino_

4 years

Probably the most brilliant marketing approach. But with this slogan I of course couldn’t resist to post it. Very thoughtful and someone is already very excited about becoming a future scientist 😍🙆🏻‍♀️ thanks Nico Clemens @thermosciEMSpec @thermofisher l

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Cristina Paulino

@CPaulino_

4 years

Finally I have a word for this persistent feeling!!

Reviewer 2

@GrumpyReviewer2

4 years

Academic in nutshell #AcademicTwitter @AcademicChatter

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Cristina Paulino

@CPaulino_

3 years

Very thoughtful congrats from my institute for the recent acquired grant 🏆 💐. Feels good and motivating to be embedded in such a supportive environment with the greatest colleagues @ResearchGbb @MembraneEnzymo1 @univgroningen

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Cristina Paulino

@CPaulino_

5 years

Did I already mention how much I love Switzerland 🇨🇭 and the mountains 🏔 and hiking 🥾? Just breathtaking and finally got to do my bday present 🚁 🏔 saw the peak of the Matterhorn. Hope perhaps one day I live again in Switzerland 🇨🇭

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Cristina Paulino

@CPaulino_

2 years

Our latest KdpFABC work is now on @eLife . Find out how an intriguing chimera between a P-type ATPase and a K+-channel, transitions into an off-cycle state to prevent toxic K+ accumulation. Great collaborative work see credits👇🏽1/2

Inhibited KdpFABC transitions into an E1 off-cycle state

The serine phosphorylated and inhibited KdpFABC complex (KdpFABS162-PC) adopts an off-cycle E1P tight state, due to an impaired E1P/E2P transition.

elifesciences.org

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Cristina Paulino

@CPaulino_

5 years

I cannot express my joy. Almost cried yesterday when entering the stylish brand new food corner on zernike campus @univgroningen . After 2 years of literary not having anything on campus that I would call food or a place to have a drink now this beauty 🍕🍔🥙🌮🥘🍲☕️ 😭😃

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Cristina Paulino

@CPaulino_

7 months

Check out our newest publication with @Lynagh_Lab and @DandamudiMowgli on a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Lead by the fantastic @VKalienkova , with all our contribution conducted in my old lab at @ResearchGbb

Structural basis for excitatory neuropeptide signaling

Nature Structural & Molecular Biology - Kalienkova et al. use cryo-electron microscopy to capture 3D structures of a neuropeptide-gated DEG/ENaC ion channel in resting and ligand-bound states,...

www.nature.com

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Cristina Paulino

@CPaulino_

5 years

😱so much bigger than I thought. I am one of 7 winners out of 170 applications 😱 feeling super thrilled and humbled to be one of the honored ones. Thanks @elife for this great support 😊🎉🍾🎤

eLife Community

@eLifeCommunity

5 years

Congratulations to @DreuxMarlene , @xavier_guell , @Lahondere_Lab , @Chantalarwen , @ocklenBLOG , @Paulino_Lab and Lauren Schroeder – recipients of our first travel grants of 2019 🌏

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Cristina Paulino

@CPaulino_

5 years

so cool cool cool cool 👩🏻‍🏫 In case you want to know a little bit more about me, here is your change 😎

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Cristina Paulino

@CPaulino_

3 years

🤩Check out our newest collaborative study on the rational design of ASCT2 inhibitors. Fantastic & interdisciplinary work with the groups of Avner @schlessingerlab @AvedaSci , Christof @eliasndaru , Max @BonomiMax , and Dirk @MembraneEnzymo1 @alisa_garaeva 👇🏽

Rational design of ASCT2 inhibitors using an integrated experimental-computational approach | PNAS

ASCT2 (SLC1A5) is a sodium-dependent neutral amino acid transporter that controls amino acid homeostasis in peripheral tissues. In cancer, ASCT2 is...

www.pnas.org

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Cristina Paulino

@CPaulino_

5 years

Our latest papers on TMEM16s are now out in their polished form. Also with a nice press release :) What a great team work! Big Thanks to @VanessaClericoM , @CarolinaAlvadia , @Gerculera and @GOostergetel and the rest of the @Paulino_Lab and Dutzler_Lab. Below the links

Science LinX-Faculty of Science and Engineering-UG

@ScienceLinX

5 years

Two papers describe how a membrane protein can move both lipids and ions via @univgroningen @EurekAlert Work by @Paulino_Lab published today in @eLife See also

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Cristina Paulino

@CPaulino_

3 years

And here now open for everyone that would want to play with the raw dataset. Current highest resolved and smallest membrane protein (2.7Å, 115kDa) determined at 200kV #CryoEM amazing work @chancie_t 🤩🥳

Chancie T - chanciet.bsky.social

@chancie_t

3 years

Finally, the raw data for our 2.7Å! reconstruction of the folate ECF #transporter (115kDa) in lipid nanodiscs by #cryoEM @ 200kV! available from @EMDB_EMPIAR EMPIAR-10942 Link to our paper: @MembraneEnzymo1 @CPaulino_ @rheinbergerj

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Cristina Paulino

@CPaulino_

6 years

My first corresponding author paper :)

NatureStructMolBiol

@NatureSMB

6 years

Cryo-EM structure of human SLC1 transporter ASCT2 in the inward-facing conformation helps to elucidate the transport cycle.

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Cristina Paulino

@CPaulino_

6 years

1/2 How a pump hijacks a channel. Amazing how nature combines its best to form a new chimeric transport mechanism. Cryo-EM structures of KdpFABC suggest a K+ transport mechanism via two inter-subunit half-channels

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Cristina Paulino

@CPaulino_

5 years

Just finished two days interviewing fantastic, bright, smart and visionary women for new faculty positions at GBB @univgroningen via our Rosalind Franklin Program. So inspiring to see such a talented set of women at once. We exist! Give us the opportunity and we are here to stay

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Cristina Paulino

@CPaulino_

2 years

A special lab outing and team building event with my group today. #carsmashing 🤜🏼 🚗 🔨 What a blast. “unleash the beast within and cleanse in the process”. The ultimate stress resale and soooo much fun!!! Thanks everyone 🤩

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Cristina Paulino

@CPaulino_

2 years

After spending my entire live moving around, this might be it. Strange but comforting thought. I guess you can't get rid of me anymore academia and may I help and support many more newcomers including #WomenInSTEM in their endeavour 💪👩🏽‍🔬👨🏽‍🔬🧪

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Cristina Paulino

@CPaulino_

2 years

What a blast tonight 🤩🥰. Xmas dinner aka farewell to my amazing postdocs @chancie_t and @VKalienkova aka early farewell for me too. All including old alumni’s from the Paulino group.

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Cristina Paulino

@CPaulino_

1 year

Our newest contribution in understanding this special class of ABC transporters. What a milestone! 🤩 Final work from our fantastic postdoc @chancie_t with Dirk Slotboom @MembraneEnzymo1 See thread below for more insights 👇🏼

Expulsion mechanism of the substrate-translocating subunit in ECF transporters

Nature Communications - The final step in an ECF transporters transport cycle involves the expulsion of the membrane embedded substrate binding protein (the S-component) from the motor (the ECF...

www.nature.com

Chancie T - chanciet.bsky.social

@chancie_t

1 year

Excited to share our work on how ECF #transporters use ATP binding to charge a molecular spring and tune the #membrane shape to expel membrane-embedded substrate-binding proteins from the docking site into the membrane. #cryoEM #Biochemistry #MDsimulation

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Cristina Paulino

@CPaulino_

4 years

Women in Biophysics website finally online. Great vision & enormous effort of @SwissIonChannel and @ProfSharona through @NCCR_TransCure . Next time you say you "don't know an excellent successful female scientist" check here👇 #WomenInScience #WomenInSTEM

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Cristina Paulino

@CPaulino_

4 years

1/x Often mentioned and now finally on paper😅. Check out our #cryoEM data collection workflow with increased efficiency, by selecting holes based on optimal ice thickness. Great work of @rheinbergerj , Gert and SerialEM implementation by @Nexperion .

Optimized data acquisition workflow by sample thickness determination

Sample thickness is a known key parameter in cryo-electron microscopy (cryo-EM) and can affect the amount of high-resolution information retained in the image. Yet, common data acquisition approaches...

www.biorxiv.org

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Cristina Paulino

@CPaulino_

3 years

Join us for this fantastic meeting on new advances in membrane protein structure and function #EMBOMPs . Great line of speakers and further selection from abstracts. Don't miss it. Register 👇

EMBL Events

@EMBLEvents

3 years

Integral membrane proteins are structurally and biochemically difficult to research. Join us to learn all aspects of a variety of tools to study the structure and function of membrane proteins. #EMBOMPs 📩Submit abstract by 4 October

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Cristina Paulino

@CPaulino_

3 years

Ugly holiday sweater day at the Paulino Lab today 🎄🧑🏻‍🎄🧑🏽‍🎄🎅🏾🤶🏼🎄🤣 Thanks guys!

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Cristina Paulino

@CPaulino_

5 years

Fantastic new work from my colleagues. Check it out! Data acquired in house on our 200kV Talos. In case you want to use our facility and get such great data too contact me!

Membrane Enzymology

@MembraneEnzymo1

5 years

Our paper presenting the structural ensemble of a glutamate transporter homologue under “turnover conditions” is out. We tried to impersonate the work in hula hoop discs with Albert (occluded), Valentina (inward open) and Dirk (outward open)

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Cristina Paulino

@CPaulino_

7 months

Thanks Basil @BJ_Greber ! This is how it should always be. Wish that all would see & act this way. The obsession for the best resolution number needs to stop. Often misleading, overestimated and simply incorrect. Map quality, the region of interest and biological question matter!

Basil Greber

@BJ_Greber

7 months

It may be worth noting that "standard" RELION 4 gave 2.9 Å resolution, cryoSPARC 3DFlex gave 2.7 Å (with a larger particle set) and RELION 5/BLUSH gave 3.0 Å. But the map quality of the latter was clearly the best, and using that map was the obvious choice.

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Cristina Paulino

@CPaulino_

4 years

Our newest work in generating and characterising a bispecific sybody pair with potent viral neutralization effect. Potential new source for a fast & cheap therapy against #COVID19 ? Great work with the fantastic @seegerlab , @MembraneEnzymo1 and Plattet.

Highly potent bispecific sybodies neutralize SARS-CoV-2

The COVID-19 pandemic has resulted in a global crisis. Here, we report the generation of synthetic nanobodies, known as sybodies, against the receptor-binding domain (RBD) of SARS-CoV-2 spike...

www.biorxiv.org

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Cristina Paulino

@CPaulino_

1 month

Check out our newest work on ASCT2. From the long lasting collaboration with my dear colleague Dirk Slotboom @MembraneEnzymo1 with the fantastic @amborowska leading the work in collaboration with the Marrink lab @CG_Martini 1/2 👇🏼

Membrane Enzymology

@MembraneEnzymo1

1 month

It took some time, but Anna’s @amborowska beautiful work on the exchange mechanism of ASCT2 is now out. Together with twitterless Gabriella and @CPaulino @alisa_garaeva @rheinbergerj @CG_Martini

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Cristina Paulino

@CPaulino_

6 years

What a career! What an enormous audience today, honoring the work of Carol Robinson who gave the 2019 Biophysical society lecture. So inspirational. #WomenInStem #bps19

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Cristina Paulino

@CPaulino_

3 years

Very well deserved. In fact long overdue! @bcarra2 Bridget Carragher is not only a great scientist but an inspirational role model and keeps rocking 🤩👩🏻‍💻

Bridget Carragher to Receive 2022 Innovation Award

ROCKVILLE, MD – The Biophysical Society is pleased to announce that Bridget Carragher, PhD, of the New York Structural Biology Center, has been named the recipient of the 2022 Innovation...

www.biophysics.org

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Cristina Paulino

@CPaulino_

5 years

Happy to be back in Frankfurt and meet old friends (Janet, Deryck, Werner, @Oezkan_YildizZ , @kudryashev_lab and and and). Find films of my 2D crystals back from my PhD. And even the old optical bench and scanner are still there. Feeling very nostalgic at the @MPIbp and Biozentrum

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Cristina Paulino

@CPaulino_

6 years

1/2 Super excited to share on BioRxiv two more great cryo-EM stories on TMEM16 lipid scramblases that nicely complement each other. First: "Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM"

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Cristina Paulino

@CPaulino_

5 years

Never been so proud at a PhD defense. @VKalienkova absolutely nailed, and made my job very easy. Best PhD talk ever! Did I already say how proud I am? Best thing she will soon join my group 😁👩🏻‍💻

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Cristina Paulino

@CPaulino_

1 year

Check out our newest contribution. Best explained by my (former) superstar postdoc @VKalienkova . Collaboration with @TimLynagh @MowgliDM . And yes sometimes #cryoEM on membrane proteins can be super fast and efficient!

Valeria Kalienkova 🇺🇦🌻

@VKalienkova

1 year

Check out our latest preprint on how FMRFamide activates neuropeptide-gated Deg/ENaCs! @CPaulino_ Collaboration with @Lynagh_Lab @MowgliDM

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Cristina Paulino

@CPaulino_

4 years

⁦And there it finally is. The chloride channel in glutamate transporters (SLC1). Congrats @renaemryan1 ⁩ and the rest of the superb team!

Glutamate transporters contain a conserved chloride channel with two hydrophobic gates

Glutamate is the most abundant excitatory neurotransmitter in the central nervous system, therefore its precise control is vital for maintaining normal brain function and preventing excitotoxicity[...

www.biorxiv.org

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Cristina Paulino

@CPaulino_

5 years

Happy to have the great Simon Newstead @inwardopen over in Groningen @MembraneEnzymo1 for a talk and as member of a PhD Defense 👨🏻‍🎓

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Cristina Paulino

@CPaulino_

3 years

Just amazing🤩 Structures of XKR scramblases, involved in apoptotic singling from the Dutzler lab. Only 43kDa big🤯 Huge compliment to my former lab, in particular to the amazing work done by @Monique86321774 , I remember how @CarolinaAlvadia started it.

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Cristina Paulino

@CPaulino_

3 years

Perfectly phrased @IHanelt . Please check out our newest contribution to understanding the enigmatic and fantastic chimeric KdpFABC complex, which transports K+ via a channel-like and P-type ATPase subunit. Backed up by functional assays, cryoEM and MD 🤩👇🏼

Inga Hänelt

@IHanelt

3 years

In collaboration with @CPaulino_ and @pstansfeld labs we are providing molecular insights for understanding ion transport through chimeric KdpFABC. Credits to @jakobsilberberg , @RobinCorey1 , @lisahielkema and @charlott_stock , who did a great job!

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Cristina Paulino

@CPaulino_

6 years

There are people who simply should have a Wiki Page, and if not we should all contribute. Thanks to my students @Alisa89274472 @VanessaClericoM @lisahielkema for the help! Have fun reading an impressive cv.

Bridget Carragher - Wikipedia

en.wikipedia.org

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Cristina Paulino

@CPaulino_

5 years

Great new opportunities for talented and inspiring female scientists to join us at the @univgroningen , NL. have a look and spread the word! ;

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Cristina Paulino

@CPaulino_

4 years

Just finsihed attending the virtual EMBO on 'Gender Bias and its Impact in Academia' #GenderAcademia2020 As expected depressing, but also very insightful!! A lot of new ideas and examples. Yet, we need more men to attend (only 13%!), spread the word and make a difference.

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Cristina Paulino

@CPaulino_

3 years

#EMBOMPs just started! Looking forward to all the fantastic talks, posters and the general program Christian Löw @AllUNeedIsLoew , Panos Kastritis @3Dstructure and Sandro Keller and I put together. See you there, and make sure to connect!

Recent advances in structural biology of membrane proteins

www.embl.org

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Cristina Paulino

@CPaulino_

5 years

Our lab is growing 😅😍

Valeria Kalienkova 🇺🇦🌻

@VKalienkova

5 years

Katie the science cat :D

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Cristina Paulino

@CPaulino_

6 years

Celebration of a new grant for the lab with my lab at my place 😎

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Cristina Paulino

@CPaulino_

3 years

Need another example of how working together is so much better and productive than trying it all by yourself? Check our collaborative work with the lab of @IHanelt and @pstansfeld with exciting new insights into the unique chimeric KdpFABC transporter. All credits listed here 👇🏼

Phill Stansfeld

@pstansfeld

3 years

Great to see @jakobsilberberg , @RobinCorey1 , @lisahielkema & @charlott_stock 's Kdp paper is now out in @NatureComms : - a very nice collaboration with @CPaulino_ & @IHanelt

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Cristina Paulino

@CPaulino_

5 years

I love the original video so much, although I still have difficulties understanding what he is actually saying it is one that makes me laugh so hard 😂 All the memes that came out of it are glorious, especially this one. Perfect to explain the insanity of the reviewing industry!

Simon M. Hoser, PhD

@GenieOfGenes

5 years

When somebody explained to me how publishing in science works, this is what it felt like not saying it's all bad but it should be better

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Cristina Paulino

@CPaulino_

5 years

Just driving back from our fantastic joint lab retreat with groups from 🇳🇱Groningen (Slotboom, Guskov, Paulino), 🇩🇪Germany (Hänelt, Geertsma) and🇨🇭Switzerland ( @seegerlab ). Lot of fun and great exchange of knowledge. Hope we keep this tradition alive for many more years!

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Cristina Paulino

@CPaulino_

3 years

#EMBOMPs now with pic👇🏽 Couldn't be more proud & thrilled to see @chancie_t show our results ( @MembraneEnzymo1 ) on the ECF transporter and the use of 200kV #cryoEM - not a screening machine! Very deserved he got to talk right after the amazing pioneers Brian Kobilka & @gonenlab

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Cristina Paulino

@CPaulino_

3 years

I still cannot believe that Europe woke up to a war 😖with one my fantastic Postdocs @Lerche91961920 being a proud Ukrainian 🇺🇦 I feel emotionally close to what is happening. Shameful! Raise awareness, acknowledge the wrongdoing and donate

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Cristina Paulino

@CPaulino_

3 years

Check our #COVID19 study out. For ~2 years on Biorxiv, making it accessible for everyone and allowing us to continuously add data to the study. But now also available peer-reviewed #CryoEM thanks to @seegerlab (sybodies rule) and the Plattet lab.

Seeger Lab

@seegerlab

3 years

📢Our rapid response to the COVID-19 pandemic finally in peer-reviewed form @emboreports in collaboration with Philippe Plattet, @CPaulino_ @MembraneEnzymo1 🥳 „Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance“

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Cristina Paulino

@CPaulino_

2 years

Nice piece from my former PhD supervisor. Remember well how the resolution revolution felt and hit us all, in fact we did not believe how good it can get back then 🤩

Max Planck Institute of Biophysics

@MPIbp

2 years

Congrats to 40 years in cryo-electron microscopy of membrane proteins! Great article by Werner Kühlbrandt who witnessed & contributed to the astonishing evolution of #cryoEM from a niche technique to one of the most important methods in #structuralbiology .

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Cristina Paulino

@CPaulino_

5 years

Just finished a fantastic visit to the @biozentrum . Thanks @leonie_anton for the superb organisation and @CPerezLab , @MaierLab , @HillerLab , @hugomh87 , @rdrighetto and others for the great insightful chats. Hope I will be able to take the Swiss-knife present on the plane

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Cristina Paulino

@CPaulino_

5 years

Great new Methods book 📚on MPs out! Check out chapter 17 on cryo-EM in nanodiscs written by the talented @VKalienkova , @VanessaClericoM & @CarolinaAlvadia . Hope you find some of our tipps and insights helpful. Thanks @CPerez_BZ & @MaierLab for putting it together. Looks great!

Camilo Perez

@CamiloPerezC6H6

5 years

Our book "Expression, Purification, and Structural Biology of Membrane Proteins" is now online @CPerez_BZ @MaierLab @biozentrum #MembraneProteins #StructuralBiology

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Cristina Paulino

@CPaulino_

4 years

Anyone really surprised? Working more hours than what your contract says is the most normal thing in academia and yet persistently (intentionally?) ignored by the government and other stakeholders to not take action. Nice to see some number though. 1/x

10000uur

@10000uur

4 years

We are a group of students who calculated that every day a shocking 10.000 hours overtime is worked by teachers of Utrecht University. On the 31st of august, we will be spraying 10.000 chalk squares through Utrecht. One for every hour unpaid labour. #10000uur #VerwijtinKrijt

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Cristina Paulino

@CPaulino_

2 years

@SjorsScheres keeps rolling and collecting awards. Very well deserved. Today the Ivano Bertini award at the 2022 @instructhub biennial structural biology conference in its almost home town Utrecht 🇳🇱. Great talk

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Cristina Paulino

@CPaulino_

6 years

My new tool to motivate and challenge my lab but above all to acknowledge and show appreciation. The "Golden Mic Trophy" will be passed around to the next person who had a substantial achievement: beat the current highest resolution in the lab; publications; or something else...

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Cristina Paulino

@CPaulino_

5 years

Great talks from my PhD students @VanessaClericoM , @Alisa89274472 and @lisahielkema today at the Dutch NVvM cryo-EM meeting. They rock and I am so proud!!! 🙆🏻‍♀️👩🏻‍🏫👩🏼‍🔬👩🏼‍💻🔬

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Cristina Paulino

@CPaulino_

3 years

And the after party with her well deserved small present. A Dutch version of our golden trophy, which she deserves to have now forever 🥰 🔬👩🏻‍🎓

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Cristina Paulino

@CPaulino_

6 years

😊 Scientists reveal structure of amino acid transporter involved in cancer via @univgroningen @EurekAlert

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Cristina Paulino

@CPaulino_

3 years

Feeling honored that our work is highlighted by Sarah Piper @PiperProteins . Biggest credit still goes to the frontman of the work @chancie_t

PiperProteinProductions

@PiperProteins

3 years

#Render of the week today features a small but impressive #membrane protein structure, solved using a 200kV electron microscope by @CPaulino_ et al! #CryoEM structure of the folate-specific ECF transporter complex in nanodiscs, PDB:7NNU and EMD-12484 rendered in #Blender3D (1/2)

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Cristina Paulino

@CPaulino_

5 years

And the food joy continues... The authentic Sicilian corner () made my Italian student @VanessaClericoM almost cry when tasting their delicious cannoli. Smth u don't share too much with others right @lisahielkema ? I guess we are all big foodies in the lab!

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Cristina Paulino

@CPaulino_

2 years

The resolution revolution continues indeed 🤩

Da-Neng Wang

@danengw

2 years

For those eager to solve #CryEM structures at 1 Å resolution, there is great news: One no longer needs to flash the cold FEG tungsten tip every 4 hrs. The labs of Jie Tang at NIMS and Lu-Chang Qin at UNC invented a LaB6 tip that shows no intensity decay over 100 hrs.

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Cristina Paulino

@CPaulino_

3 years

Another way of aiming for a more sustainable lab, you can make art of what others would trash 👨🏻‍🔬🧪🦅

Valeria Kalienkova 🇺🇦🌻

@VKalienkova

3 years

Nothing is a waste with a wizard like our EM facility manager @MarcStuart7 Second life for a measuring cylinder 🥰

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Cristina Paulino

@CPaulino_

6 years

This week our new lab member finally visited the lab 😍. After a lot of cuddling she directly started working 😂 got a spot on our wall of fame and a replica of her in glass!

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Cristina Paulino

@CPaulino_

7 years

What a perfect start for my own group! Bringing the resolution revolution to Groningen

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Cristina Paulino

@CPaulino_

3 years

Everyone attending the #BPS22 #CryoEM go and talk to @chancie_t . Great story but above all an amazing scientist to keep an eye on!! 👀👇🏼

Chancie T - chanciet.bsky.social

@chancie_t

3 years

Flying to #SFO to attend #BPS22 @BiophysicalSoc presenting our work @MembraneEnzymo1 @CPaulino_ @rheinbergerj on ECF #transporters by 200kV #cryoEM . Stop by my poster (LB13, 22Feb) and find out more 💡

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Cristina Paulino

@CPaulino_

2 years

I think this made my weekend 🤣🤣 on point although I would argue it is normally not that obvious 🤣🤣

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Cristina Paulino

@CPaulino_

4 years

Brilliantly explained! This feeling we women often have when certain compliments and "polite" behaviour feel out of place. YES THEY ARE! Benevolent sexism: a feminist comic explains how it holds women back

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Cristina Paulino

@CPaulino_

6 years

Happy New Year to everyone. That it will be even more succesfull and fun than the last one. The Paulino Lab just grew by another puppy member...

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Cristina Paulino

@CPaulino_

6 years

What’s better than to spend the end of the day with your lab, a bunch of pizza and wine and listen to @SjorsScheres (right upper corner) talking about all the new game-changing implementations in Relion3.0? Thanks @SBGrid . Great talk kicking off nice discussions in the group!

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Cristina Paulino

@CPaulino_

3 years

Today @ #EMBOMPs : Couldn't be more proud and thrilled to see @chancie_t present our results ( @MembraneEnzymo1 ) on the ECF transporter and the use of 200kV #cryoEM - not a screening machine! Very deserved he got to talk right after the amazing pioneers Brian Kobilka and @gonenlab

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Cristina Paulino

@CPaulino_

5 years

Great talk. Turns out the JEOL cryoARM works well! Looking forward to all that might come out if it.

Ariane Briegel

@BriegelAriane

5 years

@Ludo_cryoEM introducing Rouslan Efremov for the @NeCEN_2p0 seminar!

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Cristina Paulino

@CPaulino_

4 years

1/5 With great pleasure and in honour of the International Day of Women and Girls in Science, below a mini-profile of my new colleague at @ResearchGbb Kasia Tych @BiophysK , an always smiling power package! #WomenInSTEM #AcademicTwitter #AcademicChatter #WomenInScienceDay

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Cristina Paulino

@CPaulino_

1 year

Indeed this also happened 😵‍💫😏 was trying to forget it Dirk, but good to remind people that bad/inappropriate reviews still happen. Hard to endure, especially for the Postdocs and PhDs. But hey on the bright side: the editor stood by us and we ultimately got it published 🤩

Membrane Enzymology

@MembraneEnzymo1

1 year

Although we are proud of our published work, the road to publication has been demotivating for the postdocs and PhD students who scrutinised every detail of their work. Have a look at the peer review files associated with the paper, and you will see why.

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Cristina Paulino

@CPaulino_

5 years

After some feedback from people I realized I should mention that all the work coming from my lab up to now (like this one) has all been done on a 200kV Talos Arctica. So definitely not just a screening machine! 😉

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Cristina Paulino

@CPaulino_

2 years

Always happy when we can contribute to the development and testing of new systems, workflows, and software #cryoEM @thermofisher @chancie_t @rheinbergerj

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Cristina Paulino

@CPaulino_

4 years

Big light shed into the chloride conductance of SLC1A. Impressive work by @renaemryan1 , @emadtaj and colleagues. Congrats!

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Cristina Paulino

@CPaulino_

4 years

I am shocked and disgusted. While @angew_chem is investigating, a prestigious scientist actually wrote this and sb read and approved it in the first place. This is such a disgraceful, racist, stupid essay against diversity and (gender) equality #WomenInSTEM . So destructive 😱

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Cristina Paulino

@CPaulino_

2 years

I life career honor and recognition. More than deserved: Dirk Slotboom a member of the Dutch Royal Academy of Sciences

Membrane Enzymology

@MembraneEnzymo1

2 years

Dirk is very honoured with his election as member of the Royal Academy of Sciences in The Netherlands (KNAW, , @_knaw )

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Cristina Paulino

@CPaulino_

5 years

Such a great talk and so inspiring, especially as a female scientist myself 👩🏻‍🔬👩🏻‍💻👩🏻‍🎓. Extremely thrilled and lucky to have been one of the 1500 people watching @francesarnold talk at the #chains2019 meeting.

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Cristina Paulino

@CPaulino_

7 months

So incredible proud of you Valeria. @VKalienkova very much deserved! Hope this is the kickstart of you becoming a PI and a colleague forever. People watch out for this superstar 🤩

Valeria Kalienkova 🇺🇦🌻

@VKalienkova

7 months

Humbled and honoured to be awarded #MSCA Individual Fellowship to investigate the architecture of myelin sheath at @PetriKursulaLab @UiB 😊

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Cristina Paulino

@CPaulino_

11 months

8/10 Now on a more personal note, which I rarely do this way but want to this time. All of this happened during a special and vulnerable time in my life. I literally submitted the paper a few days before giving birth to my second daughter ( #womeninSTEM 💪🏽!)...

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