Jeffrey Mold @Jeff_Mold Twitter profile | Pikagi

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Jeffrey Mold

@Jeff_Mold

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American Scientist living in Sweden - Studying high risk/low reward curiosity-driven biology. All views are my own. I went to college.

Stockholm, Sweden

https://t.co/RNu09GYsdw

Joined September 2016

Don't wanna be here? Send us removal request.

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@Jeff_Mold

Jeffrey Mold

3 years

I'm excited to unveil a project close to my heart for years now on BioRxiv titled: ‘Clonally heritable gene expression imparts a layer of diversity within cell types’ or ‘How I learned to eschew heterogeneity, and love homogeneity in scRNAseq data’ 🧵1/23

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@Jeff_Mold

Jeffrey Mold

2 years

Stop renaming effector T cells just cause you found some of them express some different genes in your 10x data. It’s confusing everyone. They are just effector T cells. Not CD8+GZMB+ cells which also express Interferon genes and KIRS (CD8GINFKIR)

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@Jeff_Mold

Jeffrey Mold

8 months

Paper is out today! Took 2 years. Cell Systems was great review process all around. Spent some time at Science but editor quit mid-review and one reviewer didn't see the point 'we know cells have memory, is it just that this shows more of it?!'

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Clonally heritable gene expression imparts a layer of diversity within cell types

Mold et al. show that within a cell type, clonally related cells exhibit heritable differences in gene expression that persist for years in vivo. This form of memory impacts thousands of genes and is...

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@Jeff_Mold

Jeffrey Mold

2 years

T cells have no Hayflick Limit and most importantly: 'Our results do not reject the concept of T cell stemness, but dissociate self-renewing potential from markers previously associated with the ability to maintain longevity and proliferative fitness.'

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Functional T cells are capable of supernumerary cell division and longevity

Nature - Through iterative cycles of viral challenge and rechallenge over ten years, mouse T cells are demonstrated to have essentially infinite potential for population expansion and longevity...

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@Jeff_Mold

Jeffrey Mold

2 years

Requirement that 3 independent reviewers all have positive feelings about a study that challenges existing dogma (of which any of them may have built their careers) all but guarantees that no truly novel research will be published in competitive journals.

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@Jeff_Mold

Jeffrey Mold

2 years

I can’t wait till scRNAseq or spatial goes clinical. I can just imagine… Doctor: “I don’t know how to tell you this…” Patient: “just give it to me straight Doc” Doctor: “we found…. Heterogeneity “

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@Jeff_Mold

Jeffrey Mold

7 months

I read lots of old papers and I’ve started to really feel that “less is more”. Looking at old vs new slide decks I feel the overload of information in modern pres/papers is not translating to a proportional increase in insights. Am I alone?

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@Jeff_Mold

Jeffrey Mold

2 years

Every American that’s ever moved to Europe has at one time had the thought “if I could just find a way to bring burritos to these people I could be a millionaire “

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@Jeff_Mold

Jeffrey Mold

21 days

Time limits are stupid. Full stop. We should be promoting people with management skills and maturity to teach/mentor. Not people who can crank out a few “high impact” papers in 5-7 years. And one wonders why there’s issues with cheating and poor management/mentoring…. 🙄

@arnaud_kr

Arnaud Krebs

23 days

Congratulations to all @ERC Start grantees. Also a positive thought to the majority of applicants that were not awarded or could not apply because of the (short) time limitations after PhD! Personal take on this 👇

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@Jeff_Mold

Jeffrey Mold

1 year

Stop making me write a one sentence summary of my paper - I already did that, it is called the 'Title'. It's embarrassing to have to rewrite the title with different words and pretend like it is a new thing.

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@Jeff_Mold

Jeffrey Mold

11 months

Now you can get a Nature paper for doing 1-3 million scRNAseq cells… so if you’re planning to do as much scRNAseq as possible for your project I’d assume you get a paper in for 10-20m cells in about a year

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@Jeff_Mold

Jeffrey Mold

2 years

I have been thinking more about publishing, peer-review, and the state of academia (from a middling career scientist) and I have a possible solution I would like to explore in the coming year - follow along if you are interested, I hope to make it worthwhile! 🧵1/16

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@Jeff_Mold

Jeffrey Mold

3 years

As I prepare to teach T cell immunology this morning - I would like to reflect on the fact that one of the most influential papers in the field of T cell biology was published in the Journal of Immunology - not CNS journals.

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Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activi...

A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished. Type 1 T helper cells...

pubmed.ncbi.nlm.nih.gov

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@Jeff_Mold

Jeffrey Mold

1 year

I predict the next 10 years will see a massive overhaul of the way we understand how cancers develop and progress. For me the biggest finding of the past 5-10 years has been the finding that typical cancer mutations are likely common in healthy people

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@Jeff_Mold

Jeffrey Mold

2 years

If a highly reproducible result is published on @biorxivpreprint but languishes in peer review for a year or more. Does it 'matter' if it is peer reviewed or not? If others can reproduce it is that not a form of peer-review? Then who cares what corporate journal owns it?

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@Jeff_Mold

Jeffrey Mold

2 years

I have been immersed in the concept of ‘non-genetic individuality’ for the past few months while writing/revising papers and wanted to do a thread summarizing the field as I think it may be interesting for some! So here goes (it's LONG!): (1/27)

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@Jeff_Mold

Jeffrey Mold

7 months

The Lancet used to be so much more cool...

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@Jeff_Mold

Jeffrey Mold

3 years

Immunologists - what in particular do you think you will gain by using scRNAseq that you wouldn’t with a well designed set of FACS panels? Provide examples. Is it worth it?

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@Jeff_Mold

Jeffrey Mold

2 years

Any rule that limits the amount of time a PhD student can pursue a PhD to less than 5 years is cruel in most fields of cell/mol biology. It takes me around 1 year just to go through the publication process for 1 paper. Rules like this are incompatible with any work/life balance.

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@Jeff_Mold

Jeffrey Mold

2 years

@ctmurphy1 Unless they are a geologist

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@Jeff_Mold

Jeffrey Mold

2 years

I had no idea that my 'grinding my gears' on T cell types would resonate so much... Stick around next week and I will entertain you with: 'Those aren't new Dendritic Cells' or 'I don't think T cell clones can have 2 beta chains' or perhaps 'NK cells: Do they really exist?'

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@Jeff_Mold

Jeffrey Mold

2 months

People still are doing velocity on unrelated (not clonal defined/barcoded) cells to imply relationships despite many days of separation in putative past division times in vivo... and publishing it in Cell as main figures. In this case pooled from different mice I believe.

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@Jeff_Mold

Jeffrey Mold

2 years

I've slowly adopted the human as a model organism for understanding the complex mechanistic insights gained from mouse immunology.

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@Jeff_Mold

Jeffrey Mold

11 months

The worst thing about the single cell methods field is how often the work purports to change the way we understand biology while failing to cite decades of careful, less flashy work which these novel methods summarize and present as new. I may be guilty of it too…

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@Jeff_Mold

Jeffrey Mold

2 years

You have my attention —>

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@Jeff_Mold

Jeffrey Mold

2 years

One of the easiest and best things you can do as a scientist is to spread other peoples interesting studies around (on Twitter for example) - this is especially true for papers that aren’t in HI journals

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@Jeff_Mold

Jeffrey Mold

7 months

PhD students in biomedical research should be required to take a semester long course on human disease pathology. It’s so valuable to see what the human diseases we model look like in reality - especially commonalities and *gasp* heterogeneity (not the cool single cell kind)

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@Jeff_Mold

Jeffrey Mold

2 years

Wendell Lim publishes papers in Science like Noah brings animals on boats - in twos (and he’s a treasure and they are awesome)

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Synthetic cytokine circuits that drive T cells into immune-excluded tumors

Synthetic circuits that deliver IL-2 locally to tumors allow CAR-T cells to overcome suppressive tumor microenvironments.

www.science.org

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@Jeff_Mold

Jeffrey Mold

6 months

Things that make you go hmmmmmm

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Lifelong persistence of nuclear RNAs in the mouse brain

Genomic DNA that resides in the nuclei of mammalian neurons can be as old as the organism itself. The life span of nuclear RNAs, which are critical for proper chromatin architecture and transcription...

www.science.org

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@Jeff_Mold

Jeffrey Mold

11 months

Now this looks interesting-

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The transcriptional legacy of developmental stochasticity

Nature Communications - Minor perturbations or noise can significantly impact development but are difficult to assess. Here, the authors detail allelic expression imbalance across the development...

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@Jeff_Mold

Jeffrey Mold

1 year

Why do cancers express so many Collagen genes - I want a molecular answer - not 'because they need to remodel ECM'. There must be a common pathway upstream of these genes that gets triggered in tumours right?

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@Jeff_Mold

Jeffrey Mold

20 days

As promised here is a link to GitHub which should be a public page with my short lecture (39 slides) and 'outline/cheatsheet' - there is a ppt and pdf of the lecture

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GitHub - jeffmold/Undergrad-T-cell-Lectures: A collection of notes and slides for basic undergrad...

A collection of notes and slides for basic undergraduate introduction to T cell biology - jeffmold/Undergrad-T-cell-Lectures

@Jeff_Mold

Jeffrey Mold

20 days

I teach basic T cell immunology every Fall to 2nd year undergrads at KI with no Immunology background (other than being confused for a few weeks by @Agingfencer ). I will post a link to a place where people can have my slides and lecture outlines soon.

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@Jeff_Mold

Jeffrey Mold

2 years

The solution for everyone is staring us in the face: Step1 - submit to your favorite HI journal, Step2 - go through review, Step 3 post editorial acceptance + all reviews to bioRxiv. Step4- decline to publish. Voila - no fees

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@Jeff_Mold

Jeffrey Mold

23 days

Part 2 of what I assume is a marvel-like series of papers atlasing cancers with ever increasing expenditure on ATAC:

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Single-cell chromatin accessibility reveals malignant regulatory programs in primary human cancers

To identify cancer-associated gene regulatory changes, we generated single-cell chromatin accessibility landscapes across eight tumor types as part of The Cancer Genome Atlas. Tumor chromatin...

www.science.org

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@Jeff_Mold

Jeffrey Mold

2 years

I’ve published over 30 papers in what people consider “good” journals and on a weekly basis I wonder if the ideas/experiments I’m running at any given time are totally foolish. Never give up 😅. Doubt is totally normal

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@Jeff_Mold

Jeffrey Mold

6 months

Well this is really fun/interesting!

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A metastasis map of human cancer cell lines

Nature - A method in which pooled barcoded human cancer cell lines are injected into a mouse xenograft model enables simultaneous mapping of the metastatic potential of multiple cell lines, and...

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@Jeff_Mold

Jeffrey Mold

2 years

Every time I read a C. elegans paper I regret not having gone into that field...

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@Jeff_Mold

Jeffrey Mold

2 years

Wait Nature comms published 7500 papers in 2022 and still has an IF of 17-18. How’s that possible?!?

@PaoloCrosetto

Paolo Crosetto

2 years

These are *enormous* numbers, making IJERPH one of the largest open-access mega-journals in the world. So now you know why this is BIG news. The second largest journal in the world just lost its impact factor.

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@Jeff_Mold

Jeffrey Mold

20 days

I teach basic T cell immunology every Fall to 2nd year undergrads at KI with no Immunology background (other than being confused for a few weeks by @Agingfencer ). I will post a link to a place where people can have my slides and lecture outlines soon.

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@Jeff_Mold

Jeffrey Mold

3 years

@edyong209 @RheaBoydMD Most people I have encountered in Sweden who are hesitant to get vaccinated seem to have been scared by the message 'it can't be safe, it was too quickly developed.' It is critical to point out to all that it is built on decades of knowledge and a relative reduction in red tape

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@Jeff_Mold

Jeffrey Mold

3 years

I want to share a story of something that really impacted my career when I was a 20 year old undergrad - and I think it could be useful for young students and for powerful academics alike! 🧵

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@Jeff_Mold

Jeffrey Mold

3 years

I met with a company today that will essentially produce a cell atlas for you from frozen tissue pieces you send for a fee… including ATAC, RNA(nuclei), spatial, and all analysis. You just send frozen tissue. I kind of liked that 😊. 4-5 samples with 4-6 week turnaround.

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@Jeff_Mold

Jeffrey Mold

2 years

I think it’s unbelievably sad that my field’s flagship journal (JI) has the same IF as the predatory journals that offer me accelerated publication of my preprints despite these journals not being on topic. IF is a worthless metric #Goodhart ’s law

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@Jeff_Mold

Jeffrey Mold

1 year

After wasting an hour with google I am now convinced there is no such thing as 'cancer stem cell genes'. People just take the genes they find and run them against any stem cell lists and call the overlap CSC genes. It's the Wild West in Omics - a signature is worth nothing

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@Jeff_Mold

Jeffrey Mold

11 months

Just make sure your conclusions are “by doing more of this we envision someday we will find cool new things sans a hypothesis”

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@Jeff_Mold

Jeffrey Mold

2 years

I just discovered this paper: David Baltimore theorised how TdT would create TCR diversity years before somatic recombination was demonstrated by Tonegawa.. I want a collection of these sorts of theory papers to give to my students in the future!

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@Jeff_Mold

Jeffrey Mold

4 years

@gripemaster I am 41 and my german girlfriend just informed me that there was no opera about a deflatable mouse... :/

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@Jeff_Mold

Jeffrey Mold

1 year

Oh neat - I’ve been wondering about this…

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Y chromosome loss in cancer drives growth by evasion of adaptive immunity

Nature - Loss of the Y chromosome in tumour cells is associated with a poor prognosis for patients with bladder cancer by causing local T cell exhaustion, which also increases the response to...

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@Jeff_Mold

Jeffrey Mold

10 months

This paper is really interesting and gets at the heart of what it is to be a mature effector T cell vs an 'immature' stem cell memory T cell... I must have overlooked it but it raises some interesting philosophical points about T cell maturity.

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The epigenetic control of stemness in CD8+ T cell fate commitment

Suv39h1 silences stem/memory gene expression during effector CD8+ T cell differentiation.

www.science.org

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@Jeff_Mold

Jeffrey Mold

7 months

Damn you Janeway! Another 10 hours of revising my T cell lectures awaits…

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The immunopathological landscape of human pre-TCRα deficiency: From rare to common variants

Functional αβ T cells and late-onset immunological conditions occur in humans with rare or common inherited pre-TCRα deficiency.

www.science.org

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@Jeff_Mold

Jeffrey Mold

2 years

If you want to rejuvenate the immune system you better start by fixing the thymus. If you want to fix the thymus you better start by understanding why it disappears. If you want study that you better look at long-lived mammals where it disappears in the first quarter of life.

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@Jeff_Mold

Jeffrey Mold

2 years

I put some T cells in a well in ‘82 - threw on some cytokines just to see what they would do. I watched em differentiate and called them Th2. Oh a oh - it was a big hit, oh a oh - reviewers loved it

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@Jeff_Mold

Jeffrey Mold

3 years

I predict that in 2022 we will see a lot of immunology papers finding 'continuums' and 'spectrums' of cell identities with their new favourite Omics tools. And in 2023 we will see heterogeneous spatial organisations of these spectral populations.

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@Jeff_Mold

Jeffrey Mold

6 months

Stoner thought of the day: “what if the development of multicellular organisms required the mechanisms that give rise to tumors and our ancestors were malignant eukaryotic colonies…”

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@Jeff_Mold

Jeffrey Mold

3 years

This new paper on paternal transgenerational inheritance of immunological phenotypes in mice may seem like magic to immunologists, but it builds on decades of work in other systems and organisms showing similar things 🧵 1/21 @PhilipAhern

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@Jeff_Mold

Jeffrey Mold

3 years

@gerdosi One thing my friends who debate vaccines vs natural infection miss is the fact that natural infection likely generates a much more comprehensive and diverse repertoire of T and B cells than the RNA vaccines against spike

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@Jeff_Mold

Jeffrey Mold

2 years

A classic, but underappreciated paper characterizing the presence of activated T cells and Tregs (pre-knowledge that this is what they are) in the human fetus by arguably the greatest living immunologist Max Cooper:

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A novel subpopulation of primed T cells in the human fetus - PubMed

Virgin and memory T cells reciprocally express high levels of the RA or the RO isoforms of CD45, respectively. In an examination of T cell expression of these two CD45 isoforms during human develop...

pubmed.ncbi.nlm.nih.gov

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@Jeff_Mold

Jeffrey Mold

2 years

The first in a series of voluntary reviews of @biorxivpreprint . The paper is titled 'Plasticity and lineage commitment of individual Th1 cells are determined by stable T-bet expression quantities' from Ahmed Hegazy and Max Loehning at @DRFZ_Berlin

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Plasticity and lineage commitment of individual Th1 cells are determined by stable T-bet expression...

T helper 1 (Th1) cell identity is defined by the expression of the lineage-defining transcription factor T-bet. Here, we examine the influence of T-bet expression heterogeneity on subset plasticity...

www.biorxiv.org

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@Jeff_Mold

Jeffrey Mold

7 months

I think an excellent first year PhD course in biomedicine - 1st semester- would be to teach a history of medical research and society with required reading: “microbe hunters”, “the billion dollar molecule”, “arrowsmith”, “bad blood”, and “the emperor of all maladies”

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@Jeff_Mold

Jeffrey Mold

1 month

More cool stuff from @victora_lab with their LIPSTIC technology - this time applied to tumors and looking at DC:T cell interactions!

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Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4+ T cell...

Dendritic cells (DCs) are uniquely capable of transporting tumoral antigens to tumor-draining lymph nodes (tdLNs), and also interact with effector T cells within the tumor microenvironment (TME)...

www.biorxiv.org

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@Jeff_Mold

Jeffrey Mold

2 years

I find it humorous that the “publish or perish” model that was made an official metric of merit by the invention of the H-index will eventually destroy peer review under the weight of the number of papers people try to publish to advance their careers

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@Jeff_Mold

Jeffrey Mold

4 years

why is it breaking news that there are memory T and B cells to an acute viral infection up to a year after the infection? What is the source that says that a viral immunity can disappear after a year?!

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@Jeff_Mold

Jeffrey Mold

4 months

Interesting-

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Cellular architecture shapes the naïve T cell response

After antigen stimulation, naïve T cells display reproducible population-level responses, which arise from individual T cells pursuing specific differentiation trajectories. However, cell-intrinsic...

www.science.org

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@Jeff_Mold

Jeffrey Mold

3 years

If you guys add one more omics to the multi omics I’ll quit 😂

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@Jeff_Mold

Jeffrey Mold

2 years

Next year I am going to apply for an ERC grant just so I can come up with a ridiculous acronym and force people to talk about it. 'Spatial Transcriptomic Understanding of Pervasive Infectious Disease sequencing'

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@Jeff_Mold

Jeffrey Mold

8 months

Can we get a solid definition for what a “memory T cell” is? Is it defined by marker genes/proteins? Recall response (if so is it black/white or shades of magnitude)? Longevity post initial immune response? Persistence through multiple responses?

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@Jeff_Mold

Jeffrey Mold

2 months

Ahhh Pseudotime. What a wonderful tool

@likai_tan

Likai Tan 谭力凯

2 months

Today I learnt CD8 T cells can differentiate into NK cells or γδ T cells.

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@Jeff_Mold

Jeffrey Mold

2 months

Biorxiv works. I don’t need CNS to know this is interesting and potentially extremely important

@wgibson

William Gibson

2 months

TP53: - Discovered 45 years ago, most cited gene all time - No therapies - 500 million people currently living will die of TP53 mutant cancers without new therapies Our Preprint: - A general strategy for TP53 missense mutant cancers (majority) with prototype small molecules

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@Jeff_Mold

Jeffrey Mold

2 years

TuRD-seq (Trust us Regardless of Data) @lpachter 😉

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@Jeff_Mold

Jeffrey Mold

1 year

“Spatial mapping using four different technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells”. Just once I wish an atlas would find heterogeneity and not be surprised by it 😄

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@Jeff_Mold

Jeffrey Mold

6 months

Some labs really excel at publishing high impact papers with a ton of experiments in the p=0.01 to p=0.05 range... I am not a stats expert but how many t-tests would one expect to be in that range for a study with 30-50 statistical comparisons in their main figures...

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@Jeff_Mold

Jeffrey Mold

9 months

I don’t study fetal immunology anymore - but I used to at a time when nobody studied it. Sometimes I wish I’d continued (was told I’m stupid to stop) but now that it’s fashionable I’d like to share our most important finding - fetal and adult immune cells are different.

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@Jeff_Mold

Jeffrey Mold

2 years

I am calling it. “Spatiotemporal” is the science word of 2023.

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@Jeff_Mold

Jeffrey Mold

2 years

The standards for scientists to generate illustrations for the top journals are getting a bit out of control... (not commenting on quality of research - purely on the astonishing beauty of the illustrations herein)

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Deep Visual Proteomics defines single-cell identity and heterogeneity

Nature Biotechnology - Deep Visual Proteomics combines machine learning, automated image analysis and single-cell proteomics.

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@Jeff_Mold

Jeffrey Mold

8 months

I find studies that look at effects on short-term dietary interventions on immunity really fascinating - a big one just came out and I guess it will get a lot of attention (from a very good lab). I wonder what people think about how strong it is?

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Differential peripheral immune signatures elicited by vegan versus ketogenic diets in humans

Nature Medicine - In a post hoc multiomics analysis of a trial comparing a plant-based, low-fat diet to an animal-based, ketogenic diet, the diets have differential effects on peripheral immune...

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@Jeff_Mold

Jeffrey Mold

2 years

Did the Tabulis folks find the first Macrophages, mast cells, NK cells and ILCs with rearranged TCRs? If so I take back everything I have said in the past. This is truly novel biology. Am I wrong? Please correct me if so...

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@Jeff_Mold

Jeffrey Mold

8 months

2024 was an year of hope - armed only with 500,000USD of 10x kits and a penchant for the word 'spatiotemporal' we set off to map and chart the unknown at ever increasing resolution.

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@Jeff_Mold

Jeffrey Mold

1 year

Maybe the reproducibility crisis isn’t due to a need for a 50 page addendum with all data, reagents, and weather conditions on the day of each experiment… maybe it’s because the effect sizes aren’t all that great to begin with

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@Jeff_Mold

Jeffrey Mold

2 years

And the people bowed and prayed... to the UMAPs that they made... And R flashed out its warning - to the trajectories that were forming.. and the genes of the prophets were inscribed on the tree inference, no reference... echo - the sound - of atlas.

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@Jeff_Mold

Jeffrey Mold

3 years

@OdedRechavi Well, it probably would have been a UMAP, but he almost certainly would have invented a new way to process single beak parameters and called it FINCH-beq.

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@Jeff_Mold

Jeffrey Mold

3 years

These guys got MALAT1 in their scRNA DE analysis and just went with it 😃 'Chromatin-associated lncRNA Malat1 regulates Th17 effector program and promotes intestinal inflammation'

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Chromatin-associated lncRNA Malat1 regulates Th17 effector program and promotes intestinal inflam...

Interleukin IL-17 cytokines are central regulators of mucosal homeostasis and disease. In response to tissue injury, IL-17A promotes epithelial barrier functions and restricts local inflammation. In...

www.biorxiv.org

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@Jeff_Mold

Jeffrey Mold

8 months

The “programmed DNA elimination” during Hagfish development remains one of the coolest things I’ve learned in the genomics era…

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The hagfish genome and the evolution of vertebrates

Nature - A chromosome-scale genome assembly for the hagfish Eptatretus atami, combined with a series of phylogenetic analyses, sheds light on ancient polyploidization events that had a key role in...

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@Jeff_Mold

Jeffrey Mold

2 years

Does anyone know good examples of cancers/or “pre-cancerous” cells which actually improve the function of the tissue they reside in in a beneficial way prior to collapsing it in a malignant way?

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@Jeff_Mold

Jeffrey Mold

1 year

I love how we are expected to upload terabytes of data and code and descriptions but journals are still like - 'guy, you are going to have cut 500 words and 3 references out that explain that data, we simply cannot spare the space.'

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@Jeff_Mold

Jeffrey Mold

2 years

Wait - have people only been sequencing the exome of cancer patients? I mean this sounds interesting but how has nobody ever looked at enhancer mutations before?

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Super-enhancer hypermutation alters oncogene expression in B cell lymphoma

Nature - Active super-enhancers are highly and specifically hypermutated in 92% of diffuse large B cell lymphoma samples and display signatures of activation-induced cytidine deaminase activity,...

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@Jeff_Mold

Jeffrey Mold

1 month

Other than virtually guaranteeing funding at many places the main reason to publish in CNS is that otherwise it’s incredibly difficult to get visibility. That’s why I use this site. It’s the only way to get eyes on your work on a global scale without relying on CNS

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@Jeff_Mold

Jeffrey Mold

3 years

Paper officially out now in Cell Reports - big shout out to @jakmic2003 @joanna_hard @laurent_modolo and @FrisenJonas et al. Divergent clonal differentiation trajectories establish CD8+ memory T cell heterogeneity during acute viral infections in humans

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Divergent clonal differentiation trajectories establish CD8+ memory T cell heterogeneity during...

Mold et al. investigate the clonal architecture of the human CD8+ T cell response to vaccination with live attenuated yellow fever virus, and they demonstrate that the multifaceted T cell response to...

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@Jeff_Mold

Jeffrey Mold

2 years

Does anyone else watch nature documentaries and think 'man... I want to run RNAseq on that weird toads gonads to figure out how they can regulate reproduction based on environmental fluctuations...' Is it just me?

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@Jeff_Mold

Jeffrey Mold

2 years

Never underestimate the value of staring at cells. Look too close and they start to stare back…

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@Jeff_Mold

Jeffrey Mold

5 months

I keep seeing papers (mostly in Nature) that try to measure the density of cells in a region of UMAP space…. I guess we are still allowing this?

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@Jeff_Mold

Jeffrey Mold

1 year

@OdedRechavi “these authors contributed equally”

@buitengebieden

Buitengebieden

@buitengebieden

1 year

Branch manager and assistent to the branch manager..

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@Jeff_Mold

Jeffrey Mold

2 years

Working in a large neuroscience group I’m always impressed with the overlap of key TFs between neural stem/progenitors and T/B cells 😅. (Eomes, ezh2, sox4, bcl11a/b, gata3.. likely more!)

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@Jeff_Mold

Jeffrey Mold

4 years

I publish in @biorxivpreprint because I love writing up my work. I discuss my results openly because I enjoy productive feedback. I submit to glam journals because an old guy told me he needs a way to measure my worth and doesn’t have time to read my work @cshperspectives

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@Jeff_Mold

Jeffrey Mold

6 months

Stoner thought of the day: What if multicellularity and everything that arose from it is merely a product of the survival instincts of retroviruses…

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@Jeff_Mold

Jeffrey Mold

1 year

@AlejoFraticelli Next thing you’ll be telling me is single cell is just FACS but with more markers….

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@Jeff_Mold

Jeffrey Mold

2 years

What would happen if you made a CAR-NK cell with a TCR specific for a given antigen? CD8 T cells can express KIRs and other inhibitory receptors and presumably still use TCRs - would an NK engineered to express a TCR just be a *gasp* CD8+ T cell?!

T cell receptor engineering of primary NK cells to therapeutically target tumors and tumor immune...

Background T cell receptor (TCR)-engineered cells can be powerful tools in the treatment of malignancies. However, tumor resistance by Human Leukocyte antigen (HLA) class I downregulation can...

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@Jeff_Mold

Jeffrey Mold

2 years

Well that's good to know! Don't have to revoke that Nobel Prize from Doherty and Zinkernagel now!

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CD8 T cells contribute to vaccine protection against SARS-CoV-2 in macaques

In vivo CD8 T cell depletion in vaccinated macaques before SARS-CoV-2 challenge resulted in higher viral loads after challenge.

www.science.org

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@Jeff_Mold

Jeffrey Mold

1 year

The best thing about studying cancer gene expression is that literally every gene you google comes up as 'this gene is critical for cancer progression' AND 'this gene is protective against cancer progression'. So you just have to pick your GO strategy and can say anything ;)

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@Jeff_Mold

Jeffrey Mold

2 years

I’ve never been a fan of hiding data till papers are accepted. If I have an idea and think someone else is better situated to do it, it’s enough to see it done well. I hope that someday there’s an H-index for “good advice/inspiration”. Not all ideas are “cheap” 😉

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@Jeff_Mold

Jeffrey Mold

2 years

@aemonten Hypotheses 😅

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